Markers for disease susceptibility and targets for therapy

ABSTRACT

The invention provides novel methods for determining susceptibility for, diagnosing, and/or treating complex diseases such as autoimmune diseases, Alzheimer&#39;s disease, and schizophrenia. The novel methods are based upon the finding that the positioning of long interspersed nuclear elements (LINEs) in the genome can play an important role in the induction and progression of complex diseases. Specifically, the distance and interaction between a LINE such as the L1 element and a susceptibility gene associated with a complex diseases can provide information about the risk for the complex disease, and also provide novel targets for therapy.

[0001] This application claims priority under 35 U.S.C. §119 of U.S. application Serial No. 60/256,673, filed Dec. 19, 2000.

FIELD OF THE INVENTION

[0002] This invention pertains to complex diseases, including autoimmune diseases, methods to identify potential genes relevant to disease susceptibility, pathogenesis, and treatment; methods to determine an individual's susceptibility to be afflicted by these diseases, and methods to diagnose and treat these diseases.

BACKGROUND OF THE INVENTION

[0003] Complex diseases are those with complex and poorly understood pathogenic mechanisms and that are not attributable to a mutation in a single gene. Among the complex diseases are the autoimmune diseases, as well as diseases such as Alzheimer disease and schizophrenia. Autoimmune diseases include the prototype, systemic lupus erythematosus (SLE), as well as the organ-targeted autoimmune diseases insulin-dependent diabetes mellitus (IDDM) and multiple sclerosis (MS). It has long been understood that genetic factors play an important role in susceptibility to these diseases. With the availability of molecular tools to define the sequence of the human (and model animal) genome, extensive investigations have attempted to define the genes that confer risk of developing these diseases (1-11). Knowing the identity of genes that place an individual at risk of developing a disease may permit identification of those at-risk individuals before disease onset or early in its course, allowing early institution of treatment. Identification of those genes also should lead to new understanding of disease mechanisms, through study of the role of their gene products, and other components of their molecular pathways, in normal human physiology and in patients. The gene or genes in question may be altered, resulting in abnormal function of its protein product, or it may be produced in too much or too little quantity. Most importantly, knowledge of disease susceptibility genes may lead to the development of new therapeutic approaches based on manipulation of the expression or activity of the particular gene product or of other gene products identified through understanding the activity of the disease gene.

[0004] The importance of disease genes has led to studies to identify complex disease susceptibility loci, through “genome screens”, often using analysis of microsatellites in human DNA at spaced locations throughout the genome (1). These markers of individual variability can be statistically analyzed to determine an association or linkage to certain phenotypic traits, such as diagnosis of a particular disease or expression of a laboratory or clinical manifestation of that disease. In this way, regions of the human genome that might identify a disease gene can be narrowed down and the specific gene eventually identified (12). In spite of studies attempting to define susceptibility genes in SLE, MS, IDDM, rheumatoid arthritis (RA), Crohn's disease, and schizophrenia, among others, very few of these genes have been identified. It would therefore be highly useful to develop a method to identify potential genes important in susceptibility to or pathogenesis of diseases. Knowing the identity of such disease genes would-provide an approach to predicting who will get disease, how the disease occurs, and most importantly, will advance development of new therapies for diseases.

[0005] As the prototype systemic autoimmune disease, SLE has served as an important model to consider the genetic and environmental factors that contribute to complex diseases. The idea that viruses may trigger SLE has always been a consideration, based on the systemic symptoms that are often typical of viral infection. Viruses have been sought, most successfully in animal models of SLE. Viral particles, particularly the gp70 envelope protein characteristic of some retroviruses, have been observed in the kidneys of lupus mice and humans (16). Recent work has documented full-length copies of several classes of endogenous retroviruses in human DNA, and transcription and translation of proteins encoded by these viral parasites have been documented. A role for endogenous retroviruses with long terminal repeat (LTR) sequences has been addressed in both IDDM and SLE. In IDDM, the data are conflicting and controversial (29-32). In SLE, efforts to document viral etiologic agents have had mixed successes. Virus-like inclusion bodies have been observed in the endothelial cells of kidneys from SLE patients, and RNA or DNA with virus-like sequences has been reported (33, 34). An endogenous retroviral sequence encoded on chromosome 1q, a chromosomal location enriched in potential disease susceptibility loci, has been studied (29, 35). Some patients with SLE were shown to produce autoantibodies specific for the product of that HERV sequence. However, no well-documented story has been developed that incorporates a role for viruses or virus-like DNA sequences in the genetic susceptibility factors that underlie complex diseases.

[0006] Another class of endogenous retrovirus-like elements, retrotransposons, also has gained increasing interest and has stimulated a novel model for induction of SLE and other complex diseases presented herein. Much of the “junk” DNA that is present in the genome derives from long interspersed nuclear elements (LINEs), comprising up to 20% of mammalian genomes (36). These DNA elements are fragments of a nucleotide sequence that has been distributed at many locations throughout the genome (37,38). Unlike retroviral sequences, LINEs do not have LTR regions at the 5′ and 3′ of the element sequence. When intact, meaning containing all parts of a consensus sequence from 5′ to 3′, they contain a 5′ regulatory region and two open reading frames (ORF) that can encode two proteins. The function of LINEs is to transcribe the two ORFs into mRNA, copy that RNA (or parts of it) into DNA, and insert that DNA back into the genome (39). It has been proposed that LINEs are an important engine of evolutionary change, perhaps mediating the shuffling of exons that generates biologic complexity (40-42).

[0007] The full-length human LINE-1 (L1) element is about 6000 bp in length (see, e.g., GenBank Accession No. U09116; SEQ ID NO 1. Other full-length LINE-1 sequences include GenBank Accession Nos. U93562; U93563; U93564; U93565; U93566; U93567; U93568; U93569; U93570; U93571; U93572; U93573; U93574; AF148856; and AF149422. A nearly 900 bp 5′ untranslated regulatory region is followed by a 984 bp ORF that encodes a 40 kD protein (p40; SEQ ID NO:2) with an NH₂-terminal leucine zipper-like domain, possibly mediating protein interactions (44). For both human and murine ORF 1, the 5′ end is highly divergent (36). In common are enrichment in CpG sequences and an absence of TATA boxes (52). Several studies have investigated the 5′ regulatory motifs that are essential for effective L1 gene transcription. An important motif is found within the 5′ 30 bp of the L1 consensus sequence (53). The motif includes a G-rich sequence that binds the YY1 protein, a ubiquitous DNA binding protein that can act either as an activator or repressor. In the case of human L1, alteration of the YY1 binding site substantially reduced transcriptional activity. Of interest, additional sequences upstream of the 5′ consensus sequence also appeared to affect L1 transcription. Those sequences have neither been defined nor functionally characterized. Two additional important regulatory elements have recently been defined. Binding sites for proteins of the SOX family, located between nucleotides 472 and 477 and between nucleotides 572 and 577, have been studied (85). The male-restricted Y chromosome encoded SRY protein, the prototype of the SOX family of transcriptional regulatory proteins, binds to these two elements and inhibits LINE transcription, while other members of the SOX family bind to the same elements and increase transcription. These findings suggest that LINE transcription may be differentially regulated in males and females.

[0008] The nucleic acid binding properties of ORF1 p40 have been studied, and the protein has been shown to preferentially bind to single-stranded RNA (45). Interestingly, p40 has relative specificity for sense strand ORF2 RNA coding regions. While the function of p40 is not known, and it bears little sequence homology to known proteins, the basic COOH-domain of the protein has been mutated and shown to be essential for retrotransposition of the element in an in vitro cell culture assay. A short intervening sequence separates ORF1 from an approximately 3800 bp ORF2 coding sequence, encoding the protein represented by SEQ ID NO:3. The full-length L1 transcript, including ORF1, intron, and ORF2, is localized in cytoplasmic ribonucleoproteins (RNPs) particles with p40, and ORF2 is ultimately translated into a protein with both typical reverse transcriptase and endonuclease domains (44,46-48). As is true for ORF1 p40, both endonuclease and reverse transcriptase domains of ORF2 protein are essential for retrotransposition in vitro (49-51).

SUMMARY OF THE INVENTION

[0009] The present invention is based on the surprising discovery that the proximity of a LINE element such as L1 to a region of the genome associated with a diagnosis of a complex disease or susceptibility to a complex disease can indicate the identity of a gene or genes involved in the pathogenesis of that disease. Moreover, individual variability in the presence or nucleotide sequence of a LINE element in proximity to or within an intronic region of one or more genes associated with or involved in the development of a disease can be an indicator of an individual's susceptibility to the disease. Additionally, the detection of DNA, mRNA or protein encoded by a LINE element in the cells or body fluid of a patient with a complex disease can be used to diagnose or measure the activity of that disease, and the detection of antibodies reactive with DNA, RNA, or proteins encoded by a LINE element can be used to diagnose or measure the activity of that disease. Finally, it may be useful to inhibit the expression or activity of LINE nucleotide and protein products as a therapeutic approach in patients with complex disease. In particular, the method is applicable for complex diseases such as, e.g., autoimmune diseases, Alzheimer's disease, and schizophrenia.

[0010] Thus, the present invention provides for a method of identifying genes and gene products that are involved in susceptibility to and pathogenesis of a complex disease. Information regarding disease susceptibility loci available in the literature can be used to direct computer-based searches to a region of the genome neighboring a disease-associated marker. Comparison of the sequence of the 5′ regulatory region of a consensus L1 sequence to that genome region is used to localize full-length and full-length high fidelity L1 sequences to the intronic region of genes or predicted genes or to the 5′ or 3′ regulatory region of genes or predicted genes. Those genes containing a full-length L1 element in their intronic region or containing a full-length L1 element with high sequence fidelity to the consensus sequence in their 5′ or 3′ regulatory region are identified as potential disease genes. Alternatively, a catalogue of such genes can be generated and used as a database for study of potential disease genes relevant to various and numerous diseases. The present invention also provides for a method of identifying an individual at risk for or suffering from a complex disease, which method comprises investigating the individual's DNA in the intronic regions of genes containing full-length L1 elements or in the 5′ or 3′ regulatory regions of genes containing a full-length high fidelity consensus L1 sequence. For a given disease, a preferred method would involve directing the DNA study to those areas of the genome associated with a diagnosis of or susceptibility to that complex disease. The DNA sample can suitably be prepared from a tissue sample taken from the individual. By any method commonly used to obtain the sequence of or detect the presence of a genomic DNA segment, the region of DNA including the 5′ regulatory region of the L1 sequence and the adjacent genomic sequence are sequenced or identified. In one embodiment, the high-fidelity L1 sequence is present in the intronic region or 5′ or 3′ regulatory region of a gene in the DNA of the test individual, but not in the DNA of control individuals. In another embodiment, the sequence of the 5′ regulatory region of the L1 element in the DNA of the test individual is of higher fidelity to the L1 consensus sequence than in the DNA of control individuals. In a third embodiment, nucleotides in the 5′ regulatory region of the L1 sequence that have an important role in controlling L1 transcription will be present in the test individual but not in control individuals. Typically, the most 5′ approximately 30 nucleotides from the sequence of SEQ ID NO:1 will be identified in the context of the adjacent genomic sequence to determine the presence of a given L1 element. Alternatively, the sequence of the most 5′ approximately 884 nucleotides of SEQ ID NO:1, or another consensus L1 sequence, will be compared with the corresponding L1 sequence in the DNA of the test individual and control individuals. In one embodiment, a full-length L1 element in the intronic region of a gene has sequence identity to a consensus sequence, as that of SEQ ID NO:1, ranging from 75-100% and includes the full nucleotide sequence, or is only absent up to the first 20 nucleotides of the consensus sequence. In another embodiment, a high-fidelity L1 sequence in the intronic region or in the 5′ or 3′ regulatory region of a gene can be at least about 97% similar to the sequence of nucleotides 1-884 of SEQ ID NO:1, or, alternatively, identical to residues 1-884 of SEQ ID NO:1. In another embodiment, the DNA of the test individual will have a nucleotide alteration in a putative regulatory region contained within residues 1-884 of SEQ ID NO:1. The method is applicable for a variety of complex diseases, including systemic lupus erythematosus (SLE), multiple sclerosis (MS), insulin-dependent diabetes mellitus (IDDM), rheumatoid arthritis (RA), phemphigus, psoriasis, autoimmune thyroid disease, scleroderma, mixed connective tissue disease, polymyositis, dermatomyositis, Sjögren's syndrome, pemphigoid, vitiligo, primary biliary cirrhosis, chronic active hepatitis, Crohn's disease, ulcerative colitis, pernicious anemia, schizophrenia, and Alzheimer disease.

[0011] In addition, the invention provides for a method of identifying an individual susceptible to or at risk for or with activity of a complex disease by detecting the level of L1 DNA, mRNA or a protein encoded by an L1 element in the tissue, cell, or body fluid sample taken from the individual, wherein the individual is susceptible to or at risk for or currently affected by the complex disease if the level is higher than the level in a control sample. The tissue, cell, or body fluid sample can be taken from blood, serum, saliva, urine, tears, sweat, synovial fluid, cerebrospinal fluid, or from a solid tissue. The L1 DNA is preferably detected in a body fluid and is at least 80% identical to SEQ ID NO:1. L1 mRNA is preferably complementary to SEQ ID NO:1, or to a sequence preferably at least 95%, homologous to SEQ ID NO:1 and extending to within 20 nucleotides, preferably 10 nucleotides, of the 5′ end of a consensus sequence identical to SEQ ID NO:1. A protein encoded by an L1 element can be encoded by ORF1 or ORF2 of a sequence preferably at least 95% homologous to SEQ ID NO:1. The L1mRNA may be part of a ribonucleoprotein, and the protein encoded by an L1 element can be either ORF1 and ORF2, or a combination of both.

[0012] Furthermore, the invention provides for a method to identify an individual susceptible to or at risk for or with activity of a complex disease by detecting antibodies to DNA or RNA with at least 80% sequence identity to SEQ ID NO:1 or by detecting antibodies to the protein products of an L1 element. The antibodies for the L1 protein product can bind to the protein encoded by either ORF1 and ORF2, or a combination of both, and they may detect DNA, RNA, or ORF1 or ORF2 proteins that are part of a ribonucleoprotein particle.

[0013] Furthermore, the invention provides for a method of treating or preventing a complex disease, comprising administering a therapeutically effective amount of an agent such as an L1 antisense oligonucleotide, an agent that inhibits the transcription of L1 mRNA, an antibody directed against L1 mRNA, and/or an antibody or other molecule directed against a protein encoded by an L1 element.

[0014] In one aspect, the present invention provides a method of identifying a gene involved in a complex disease comprising the steps of identifying a region of the genome neighboring a disease-associated marker; comparing the sequence of the 5′ regulatory region of a consensus L1 sequence to the intronic region of genes or predicted genes or to the 5′ or 3′ regulatory region of genes or predicted genes; and identifying genes containing a full-length L1 element in their intronic region or containing a full-length L1 element with high sequence fidelity to the L1 consensus sequence in their 5′ or 3′ regulatory region, wherein said genes identified in step (iii) are involved in a complex disease.

[0015] In another aspect, the present invention provides a method of identifying an individual at risk for or suffering from a complex disease comprising the steps of providing a sample from the individual; identifying intronic regions of genes containing full-length L1 elements or in 5′ or 3′ regulatory regions of genes containing a full-length high fidelity consensus L1 sequence of the individual's DNA from the sample; and comparing said intronic regions of genes or said 5′ or 3′ regulatory regions of step (ii) with a control sample of DNA taken from an individual not susceptible to or at risk for or currently suffering from a complex disease wherein said genes identified in step (ii) are involved in a complex disease.

[0016] In yet another aspect, the present invention provides a method of identifying an individual at risk for or suffering from a complex disease comprising the steps of providing a sample from the individual suffering from a complex disease; detecting the amount of L1 DNA, mRNA or a protein encoded by an L1 element in the sample; and comparing the amount of step (ii) with an amount of L1 DNA, mRNA or a protein obtained from an individual not susceptible to or at risk for or suffering from a complex disease, wherein if the amount detected in the sample obtained from the individual is greater than the amount of the control, the individual is at risk for or suffering from a complex disease.

[0017] In a further aspect, the present invention provides A method for identifying an individual at risk for or suffering from a complex disease comprising the steps of providing a sample obtained from the individual; detecting antibodies directed against ribonucleo-protein particles having L1 mRNA complements in the sample wherein the individual is at risk for or is suffering from a complex disease if the antibodies are present in the sample.

[0018] In yet a further aspect, the present invention provides a method of identifying an individual at risk for or suffering from a complex disease comprising the steps of providing a sample obtained from the individual; analyzing the sample for the presence of auto antibodies directed against L1 DNA, nRNA or protein products wherein the individual is at risk for or suffering from a complex disease if the antibodies are present in the sample.

[0019] These and other aspects of the present invention will be apparent to those of ordinary skill in the art in light of the present specification, claims and drawings.

BRIEF DESCRIPTION OF THE DRAWINGS

[0020]FIG. 1. This figure shows the DNA sequence of the primer pairs for PCR amplification of an L1 element on human chromosome 1q. Nucleotides 15721 to 14892 (SEQ ID NO:4) of BAC clone AL162431 were analyzed to identify nucleotide sequences of primary 5′ and 3′ PCR primers (solid lines) and secondary nested 5′ and 3′ primers (dotted lines), shown bracketed, for amplification of a chromosomal segment that is specific to the chromosome 1q location 5′ to the L1 sequence, along with the adjacent 5′ regulatory region of the L1 element. 5′ primary and secondary nested primers are identical to the indicated sequences. 3′ primary and secondary nested primers are complementary to the indicated sequences.

[0021]FIG. 2. This figure shows that SLE susceptibility loci with high LOD scores are associated with proximity to full-length, high fidelity L1 elements or full-length L1 elements within the coding sequences of genes on chromosome 1q. The location of L1 elements is indicated with a bar, and a free-hand drawing replicating the data from microsatellite analysis of SLE susceptibility loci, derived from reference 4, is superimposed on the figure representing chromosome 1q.

[0022]FIG. 3. This figure shows that SLE susceptibility loci with high LOD socres are associated with proximity to full-length, high fidelity L1 elements or full-length L1 elements within the coding sequences of genes on chromosome 16. The location of L1 elements is indicated with a bar, and a free-hand drawing replicating the data from microsatellite analysis of SLE susceptibility loci, derived from reference 4, is superimposed on the figure representing chromosome 16.

[0023]FIG. 4. This figure shows that 3 genes on chromosome 21 contain full-length L1 elements in their coding regions. The location of L1 elements is indicated with a bar, and a free-hand drawing replicating the data from microsatellite analysis of SLE susceptibility loci, derived from reference 4, is superimposed on the figure representing chromosome 21.

[0024]FIG. 5. This figure shows expression of L1 ORF1 mRNA in NTERA-D1 cells. NTERA and HeLa cell line cells were cultured for 48 h with medium or with 5-azacytidine (5-Aza) at 0.5, 1, or 5 micromolar. Total RNA was isolated, reverse transcribed, and amplified in a competitive PCR assay for L1 ORF1 mRNA. 1 ml of cDNA, 1 ml of each of three concentrations of an ORF1-containing MIMIC (20, 10, and 5 attomoles/ml; generated using the Clontech MIMIC construction kit), 0.5 ml of ORF1-specific primers, and 22.5 ml of PCR super mix (Life Technologies, Gaithersburg, Md.) were combined and PCR was carried out by denaturing at 94° C. for 45 sec, annealing at 55° C. for 45 sec, and with extension at 72° C. for 1 min. The dilution of mimic which produced a band of equal intensity to that of target cDNA was determined. The 3 mimic concentrations are shown sequentially across the gel in triplicate FIGS. 6(A, B and C). This figure shows Western blot analysis of L1 ORF1 p40 protein. (A) Total cellular extracts were prepared from NTERA-D1 and HeLa cell line cells. Extracts were enriched in RNP particles by centrifugation at 160,000 g for 2.5 h. Proteins (50 microg/lane) were resolved on a 10% gel, transferred to an Immobilon-P membrane and immunoblotted with 1:1000 rabbit anti-p40 antibody. (B) T and non-T cells were fractionated from peripheral blood isolated from an SLE patient. The RNP fraction was isolated, 10 mg protein loaded per lane, and resolved proteins immunoblotted with rabbit anti-p40 antibody. T and non-T cells were fractionated from peripheral blood samples from three SLE patients and one healthy control individual. (C) Cell protein extracts were prepared, 50 mg protein loaded per lane and electrophoresed, and the resolved proteins immunoblotted with rabbit anti-p40antibody. The bands corresponding to the 40 kD ORF1 protein and a non-specific band at 95 kD are indicated by arrows.

[0025]FIG. 7. Western blot analysis of sera from SLE patients, healthy controls, a lupus mouse, and a control mouse. Recombinant human L1 ORF1 p40 protein was electrophoresed, transferred to a nitrocellulose filter, and then overlayed with sera . Antibody reactive with the p40 L1 protein is detected in sera from the MRL/lpr mouse, several SLE sera, and faintly in one control serum sample.

DETAILED DESCRIPTION OF THE INVENTION

[0026] All patent applications, patents and literature references cited herein are hereby incorporated by reference in their entirety.

[0027] The present invention is directed to the use of endogenous DNA elements with sequence properties of viruses, but that do not meet the definition of true viruses, that are involved in the development of “complex” diseases such as, but not limited to systemic autoimmune diseases, organ-specific autoimmune diseases, SLE, Alzheimer disease, and schizophrenia. In a preferred embodiment of the present invention, the endogenous DNA elements are LINE retrotransposons.

[0028] The present invention further provides a method for evaluating L1 elements as markers of disease genes, susceptibility factors, pathogenic triggers or mediators of complex diseases, including systemic and organ targeted autoimmune diseases. Additionally, the present invention discloses the use of L1 elements and their products as therapeutic targets in systemic and organ targeted autoimmune diseases and other complex diseases.

Definitions

[0029] As used herein, “complex diseases” are defined as multigenic diseases characterized by complex and poorly understood pathogenic mechanisms. Non-limiting examples of complex diseases include SLE, MS, IDDM, RA, psoriasis, autoimmune thyroid disease, scleroderma, mixed connective tissue disease, polymyositis, dermatomyositis, Sjögren's syndrome, pemphigoid, pemphigus vulgaris, pemphigus foliaceus, vitiligo, primary biliary cirrhosis, chronic active hepatitis, Crohn's disease, ulcerative colitis, pernicious anemia, schizophrenia, and Alzheimer disease. An individual “at risk for”, “predisposed to”, or “susceptible to” a disease or condition means that the risk for the individual to contract or develop the disease or condition is higher than in the average population.

[0030] A “high fidelity” L1 element means a sequence that shows at least about 97%, about 98%, about 99%, or up to about 100% sequence homology to a consensus L1 element or sequence, preferably a human consensus L1 element. A “moderate fidelity” L1 element means a sequence that shows at least about 75%, about 80%, about 85%, about 90%, or about 95% sequence homology to a consensus L1 sequence.

[0031] A “consensus sequence” is the sequence that reflects the most common choice of base or amino acid at each position of a series of related DNA, RNA or protein sequences. Areas of particularly good agreement frequently, although not necessarily, represent conserved functional domains. SEQ ID NO:1 is denoted as an L1 consensus sequence, or consensus element, herein.

[0032] A “consensus L1 element” can comprise at least about 30, about 200, about 400, about 600, about 800, or about 1000 nucleotide residues of an L1 element, and is preferably derived from the 5′ regulatory region. A preferred L1 element consensus sequence is a sequence derived from or corresponding to GenBank Accession No. U09116 (SEQ ID NO:1). In one embodiment, the L1 consensus sequence comprises, at least, about 30, about 200, about 400, about 600, about 800, or about 1000 nucleotides of the first (5′) 1000 or 2000 nucleotides of SEQ ID NO:1. In a preferred embodiment, the L1 consensus sequence comprises nucleotides 1-884 of SEQ ID NO:1. In another preferred embodiment, the L1 consensus sequence comprises the full-length 5′ regulatory region and approximately 5′ one third of the 5′ ORF1 sequence.

[0033] A “susceptibility locus” for a particular disease is a sequence or gene locus implicated in the initiation or progression of the disease. The susceptibility locus can be, for example, a gene or a microsatellite repeat, as identified by a microsatellite marker, or can be identified by a defined single nucleotide polymorphism. The specific genes associated with most susceptibility loci have not been identified, although many putative disease genes have been investigated. Examples of complex disease/proposed susceptibility gene locus pairs include: Graves disease/thyroid stimulating hormone receptor; primary biliary cirrhosis/S P100; pemphigus vulgaris or foliaceus/desmoglein 1 or 3; vitiligo/tyrosinase related protein 2; SLE/FcgRIIb; Alzheimer disease/APP; schizophrenia/DISC1 and CHRNA7; IDDM/insulin. Various disease susceptibility markers for SLE are also provided in Table 1 and for schizophrenia in Table 2.

[0034] Generally, susceptibility genes implicated in specific diseases and their loci can be found in scientific publications, but may also be determined experimentally. For purposes of the present invention, the “locus” of a susceptibility gene refers to the most 5′ nucleotide in the coding sequence for the susceptibility gene. As the sequencing of the human genome is still in progress, precise locations and DNA sequences of genes and disease loci remain subject to revision pending completion of the full genome analysis in multiple individuals.

[0035] A “microsatellite repeat” or “microsatellite” can also be an indicator to “susceptibility” of certain complex diseases, such as Crohn's disease, schizophrenia, and SLE as described herein. The term “microsatellite repeat” refers to a short sequence of repeating nucleotides within a nucleic acid. Typically, a microsatellite repeat comprises a repeating sequence of two (i.e., a dinucleotide repeat), three (i.e., a trinucleotide repeat), four (i.e., a tetranucleotide repeat) or five (i.e., a pentanucleotide repeat) nucleotides. Microsatellites of the invention therefore have the general formula (N₁, N₂, . . . N_(i))_(n), wherein N represents a nucleic acid residue (e.g., adenine, thymine, cytosine or guanine), “i” represents the number of the last nucleotide in the microsatellite, and “n” represents the number of times the motif is repeated in the microsatellite locus. In one embodiment the number of nucleotides in a microsatellite motif “i” is about six, preferably between two and five, and more preferably two, three or four. The total number of repeats “n” in a microsatellite repeat may be, e.g., from one to about 60, preferably from 4 to 40, and more preferably from 10 to 30 when i=2; is preferably between about 4-25, and more preferably between about 6-22 when i=3; and is preferably between about 4-15, and more preferably between about 5-10 when i=4.

[0036] A “control”, “control value” or “reference value” in an assay is a value used to detect an alteration in, e.g., transcriptional activity of a gene, levels of a protein or mRNA detected in a sample taken from a patient or measured in a reconstituted system, or any other assays described herein. For instance, the presence or expression of an L1 element can be tested or verified by measuring the levels of mRNA or ORF protein in a tissue sample from an individual at risk and compare the results to a control. In addition, modulation, i.e., up- or down-regulation, of the transcriptional activity of an L1 element or the inhibitory/stimulatory effect of an agent on modulation can be evaluated by comparing the measured value of transcriptional activity to that of a control value. The control or reference value may be, e.g., a predetermined reference value, or may be determined experimentally. For example, in such an assay, a control or reference may be, e.g., the transcriptional activity of a gene in the absence of an agent (to comparison with transcriptional activity in the presence of the agent); or any other suitable control or reference. In a diagnostic assay, a reference or control value may be obtained by comparing e.g., a nucleotide sequence, or a nucleotide or protein level measured, in a sample taken from a patient predisposed to or suspected of suffering from, a disease, to a corresponding sequence or measured value of a sample taken from a healthy, or “control” individual.

General

[0037] A “sample” refers to a biological material which can be tested for the presence of L1 elements. Such samples can be obtained from subjects, such as humans and non-human animals, and include tissue, especially glands, biopsies, blood and blood products; plural effusions; cerebrospinal fluid (CSF); ascites fluid; and cell culture.

[0038] The term “ability to elicit a response” includes the ability of a ligand to agonize or antagonize activity.

[0039] The term “transformed cell” refers to a modified host cell that expresses a functional protein expressed from a vector encoding the protein of interest. Any cell can be used, but preferred cells are mammalian cells.

[0040] A “test compound” is any molecule, that can be tested for its ability to modulate L1 expression and/or activity.

Molecular Biology

[0041] In accordance with the present invention there may be employed conventional molecular biology, microbiology, and recombinant DNA techniques within the skill of the art. Such techniques are explained fully in the literature. See, e.g., Sambrook, Fritsch & Maniatis, Molecular Cloning: A Laboratory Manual, Second Edition (1989) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (herein “Sambrook et al., 1989”); DNA Cloning: A Practical Approach, Volumes I and II (D. N. Glover ed. 1985); Oligonucleotide Synthesis (M. J. Gait ed. 1984); Nucleic Acid Hybridization (B. D. Hames & S. J. Higgins eds. (1985)); Transcription And Translation (B. D. Hames & S. J. Higgins, eds. (1984)); Animal Cell Culture (R. I. Freshney, ed. (1986)); Immobilized Cells And Enzymes (IRL Press, (1986)); B.Perbal, A Practical Guide To Molecular Cloning (1984); F. M. Ausubel et al. (eds.), Current Protocols in Molecular Biology, John Wiley & Sons, Inc. (1994).

[0042] A “nucleic acid molecule” refers to the phosphate ester polymeric form of ribonucleosides (adenosine, guanosine, uridine or cytidine; “RNA molecules”) or deoxyribonucleosides (deoxyadenosine, deoxyguanosine, deoxythymidine, or deoxycytidine; “DNA molecules”), or any phosphoester analogs thereof, such as phosphorothioates and thioesters, in either single stranded form, or a double-stranded helix. Double stranded DNA-DNA, DNA-RNA and RNA-RNA helices are possible. The term nucleic acid molecule, and in particular DNA or RNA molecule, refers only to the primary and secondary structure of the molecule, and does not limit it to any particular tertiary forms. Thus, this term includes double-stranded DNA found, inter alia, in linear (e.g., restriction fragments) or circular DNA molecules, plasmids, and chromosomes. In discussing the structure of particular double-stranded DNA molecules, sequences may be described herein according to the normal convention of giving only the sequence in the 5′ to 3′ direction along the nontranscribed strand of DNA (i.e., the strand having a sequence homologous to the mRNA). A “recombinant DNA molecule” is a DNA molecule that has undergone a molecular biological manipulation.

[0043] A “polynucleotide”, “nucleotide sequence”, or “oligonucleotide” is a series of nucleotide bases (also called “nucleotides”) in DNA and RNA, and means any chain of two or more nucleotides. A nucleotide sequence typically carries genetic information, including the information used by cellular machinery to make proteins and enzymes. These terms include double or single stranded genomic and cDNA, RNA, any synthetic and genetically manipulated polynucleotide, and both sense and anti-sense polynucleotide (although only sense stands are being represented herein). This includes single- and double-stranded molecules, i.e., DNA-DNA, DNA-RNA and RNA-RNA hybrids, as well as “protein nucleic acids” (PNA) formed by conjugating bases to an amino acid backbone. This also includes nucleic acids containing modified bases, for example thio-uracil, thio-guanine and fluoro-uracil.

[0044] An oligonucleotide comprising at least 10, preferably at least 15, and more preferably at least 20 nucleotides, preferably no more than 100 nucleotides, can be hybridizable to a genomic DNA molecule, a cDNA molecule, or an mRNA molecule encoding a gene, mRNA, cDNA, or other nucleic acid of interest. Oligonucleotides can be labeled, e.g., with ³²P-nucleotides or nucleotides to which a label, such as biotin, has been covalently conjugated. In one embodiment, a labeled oligonucleotide can be used as a probe to detect the presence of a nucleic acid. In another embodiment, oligonucleotides (one or both of which may be labeled) can be used as PCR primers, either for cloning full length or a fragment of L1, or to detect the presence of nucleic acids encoding L1. In a further embodiment, an oligonucleotide of the invention can form a triple helix with a L1 DNA molecule. Generally, oligonucleotides are prepared synthetically, preferably on a nucleic acid synthesizer. Accordingly, oligonucleotides can be prepared with non-naturally occurring phosphoester analog bonds, such as thioester bonds, etc.

[0045] The present invention also provides antisense nucleic acids (including ribozymes), which may be used to inhibit expression of L1 elements of the invention. An “antisense nucleic acid” is a single stranded nucleic acid molecule which, on hybridizing under cytoplasmic conditions with complementary bases in an RNA or DNA molecule, inhibits the latter's role. If the RNA is a messenger RNA transcript, the antisense nucleic acid is a countertranscript or mRNA-interfering complementary nucleic acid. As presently used, “antisense” broadly includes RNA-RNA interactions, RNA-DNA interactions, ribozymes and RNase-H mediated arrest. Antisense nucleic acid molecules can be encoded by a recombinant gene for expression in a cell (e.g., U.S. Pat. Nos. 5,814,500; 5,811,234), or alternatively they can be prepared synthetically (e.g., U.S. Pat. No. 5,780,607).

[0046] As used herein, “sequence-specific oligonucleotides” refers to related sets of oligonucleotides that can be used to detect allelic variations or mutations in the L1 element.

[0047] “Amplification” of DNA as used herein denotes the use of polymerase chain reaction (PCR) to increase the concentration of a particular DNA sequence within a mixture of DNA sequences. For a description of PCR see Saiki et al., Science, 239:487, 1988.

[0048] Specific non-limiting examples of synthetic oligonucleotides envisioned for this invention include oligonucleotides that contain phosphorothioates, phosphotriesters, methyl phosphonates, short chain alkyl, or cycloalkyl intersugar linkages or short chain heteroatomic or heterocyclic intersugar linkages. Most preferred are those with CH₂—NH—O—CH₂, CH₂—N(CH₃)—O—CH₂, CH₂—O—N(CH₃)—CH₂, CH₂—N(CH₃)—N(CH₃)—CH₂ and O—N(CH₃)—CH₂—CH₂ backbones (where phosphodiester is O—PO₂—O—CH₂). U.S. Pat. No. 5,677,437 describes heteroaromatic olignucleoside linkages. Nitrogen linkers or groups containing nitrogen can also be used to prepare oligonucleotide mimics (U.S. Pat. Nos. 5,792,844 and No. 5,783,682). U.S. Pat. No. 5,637,684 describes phosphoramidate and phosphorothioamidate oligomeric compounds. Also envisioned are oligonucleotides having morpholino backbone structures (U.S. Pat. No. 5,034,506). In other embodiments, such as the peptide-nucleic acid (PNA) backbone, the phosphodiester backbone of the oligonucleotide may be replaced with a polyamide backbone, the bases being bound directly or indirectly to the aza nitrogen atoms of the polyamide backbone (82). Other synthetic oligonucleotides may contain substituted sugar moieties comprising one of the following at the 2′ position: OH, SH, SCH3, F, OCN, O(CH₂)_(n)NH₂ or O(CH₂)_(n)CH₃ where n is from 1 to about 10; C₁ to C₁₀ lower alkyl, substituted lower alkyl, alkaryl or aralkyl; Cl; Br; CN; CF3; OCF₃; O-; S-, or N-alkyl; O-, S-, or N-alkenyl; SOCH₃; SO₂CH₃; ONO₂; NO₂; N₃; NH₂; heterocycloalkyl; heterocycloalkaryl; aminoalkylamino; polyalkylamino; substitued silyl; a fluorescein moiety; an RNA cleaving group; a reporter group; an intercalator; a group for improving the pharmacokinetic properties of an oligonucleotide; or a group for improving the pharmacodynamic properties of an oligonucleotide, and other substituents having similar properties. Oligonucleotides may also have sugar mimetics such as cyclobutyls or other carbocyclics in place of the pentofuranosyl group. Nucleotide units having nucleosides other than adenosine, cytidine, guanosine, thymidine and uridine, such as inosine, may be used in an oligonucleotide molecule.

[0049] The polynucleotides herein may be flanked by natural regulatory (expression control) sequences, or may be associated with heterologous sequences, including promoters, internal ribosome entry sites (IRES) and other ribosome binding site sequences, enhancers, response elements, suppressors, signal sequences, polyadenylation sequences, introns, 5′- and 3′-non-coding regions, and the like. The nucleic acids may also be modified by many means known in the art. Non-limiting examples of such modifications include methylation, “caps”, substitution of one or more of the naturally occurring nucleotides with an analog, and internucleotide modifications such as, for example, those with uncharged linkages (e.g., methyl phosphonates, phosphotriesters, phosphoroamidates, carbamates, etc.) and with charged linkages (e.g., phosphorothioates, phosphorodithioates, etc.). Polynucleotides may contain one or more additional covalently linked moieties, such as, for example, proteins (e.g., nucleases, toxins, antibodies, signal peptides, poly-L-lysine, etc.), intercalators (e.g., acridine, psoralen, etc.), chelators (e.g., metals, radioactive metals, iron, oxidative metals, etc.), and alkylators. The polynucleotides may be derivatized by formation of a methyl or ethyl phosphotriester or an alkyl phosphoramidate linkage. Furthermore, the polynucleotides herein may also be modified with a label capable of providing a detectable signal, either directly or indirectly. Exemplary labels include radioisotopes, fluorescent molecules, biotin, and the like.

[0050] A “coding sequence” or a sequence “encoding” an expression product, such as a RNA, polypeptide, protein, or enzyme, is a nucleotide sequence that, when expressed, results in the production of that RNA, polypeptide, protein, or enzyme, i.e., the nucleotide sequence encodes an amino acid sequence for that polypeptide, protein or enzyme. A coding sequence for a protein may include a start codon (usually ATG) and a stop codon.

[0051] The term “gene”, also called a “structural gene” means a DNA sequence that codes for or corresponds to a particular sequence of amino acids which comprise all or part of one or more proteins or enzymes, and may or may not include introns and regulatory DNA sequences, such as promoter sequences, 5′-untranslated region, or 3′-untranslated region which affect for example the conditions under which the gene is expressed. Some genes, which are not structural genes, may be transcribed from DNA to RNA, but are not translated into an amino acid sequence. Other genes may function as regulators of structural genes or as regulators of DNA transcription.

[0052] A “promoter sequence” is a DNA regulatory region capable of binding RNA polymerase in a cell and initiating transcription of a downstream (3′ direction) coding sequence. For purposes of defining the present invention, the promoter sequence is bounded at its 3′ terminus by the transcription initiation site and extends upstream (5′ direction) to include the minimum number of bases or elements necessary to initiate transcription at levels detectable above background. Within the promoter sequence will be found a transcription initiation site (conveniently defined for example, by mapping with nuclease S1), as well as protein binding domains (consensus sequences) responsible for the binding of RNA polymerase.

[0053] An “intron” is a non-coding sequence of DNA within a gene, that is transcribed into hnRNA but is then cut out by RNA splicing in the nucleus, leaving a mature mRNA that is then translated in the cytoplasm. Introns are poorly conserved and of variable length, but the regions at the ends are self complementary, allowing a hairpin structure to form naturally in the hnRNA, this is the cue for removal by RNA splicing. Introns are thought to play an important role in allowing rapid evolution of proteins by exon shuffling. Genes may contain as many as 80 introns.

[0054] An “exon” is a sequences of the primary RNA transcript (or the DNA that encodes them) that exits the nucleus as part of a messenger RNA molecule. In the primary transcript neighboring exons are separated by introns.

[0055] A coding sequence is “under the control of” or “operatively associated with” transcriptional and translational control sequences in a cell when RNA polymerase transcribes the coding sequence into mRNA, which is then trans-RNA spliced (if it contains introns) and translated, in the case of mRNA, into the protein encoded by the coding sequence.

[0056] The terms “express” and “expression” mean allowing or causing the information in a gene or DNA sequence to become manifest, for example producing a protein by activating the cellular functions involved in transcription and translation of a corresponding gene or DNA sequence. A DNA sequence is expressed in or by a cell to form an “expression product” such as a protein. The expression product itself, e.g. the resulting protein, may also be said to be “expressed” by the cell. An expression product can be characterized as intracellular, extracellular or secreted. The term “intracellular” means something that is inside a cell. The term “extracellular” means something that is outside a cell. A substance is “secreted” by a cell if it appears in significant measure outside the cell, from somewhere on or inside the cell.

[0057] The terms “vector”, “cloning vector” and “expression vector” mean the vehicle by which a DNA or RNA sequence (e.g. a foreign gene) can be introduced into a host cell, so as to transform the host and promote expression (e.g. transcription and translation) of the introduced sequence. Vectors include plasmids, phages, viruses, etc.; they are discussed in greater detail below.

[0058] Vectors typically comprise the DNA of a transmissible agent, into which foreign DNA is inserted. A common way to insert one segment of DNA into another segment of DNA involves the use of enzymes called restriction enzymes that cleave DNA at specific sites (specific groups of nucleotides) called restriction sites. A “cassette” refers to a DNA coding sequence or segment of DNA that codes for an expression product that can be inserted into a vector at defined restriction sites. The cassette restriction sites are designed to ensure insertion of the cassette in the proper reading frame. Generally, foreign DNA is inserted at one or more restriction sites of the vector DNA, and then is carried by the vector into a host cell along with the transmissible vector DNA. A segment or sequence of DNA having inserted or added DNA, such as an expression vector, can also be called a “DNA construct.” A common type of vector is a “plasmid”, which generally is a self-contained molecule of double-stranded DNA, usually of bacterial origin, that can readily accept additional (foreign) DNA and which can readily introduced into a suitable host cell. A plasmid vector often contains coding DNA and promoter DNA and has one or more restriction sites suitable for inserting foreign DNA. Coding DNA is a DNA sequence that encodes a particular amino acid sequence for a particular protein or enzyme. Promoter DNA is a DNA sequence which initiates, regulates, or otherwise mediates or controls the expression of the coding DNA. Promoter DNA and coding DNA may be from the same gene or from different genes, and may be from the same or different organisms. A large number of vectors, including plasmid and fungal vectors, have been described for replication and/or expression in a variety of eukaryotic and prokaryotic hosts. Non-limiting examples include pKK plasmids (Clonetech), pUC plasmids, pET plasmids (Novagen, Inc., Madison, Wis.), pRSET or pREP plasmids (Invitrogen, San Diego, Calif.), or pMAL plasmids (New England Biolabs, Beverly, Mass.), and many appropriate host cells, using methods disclosed or cited herein or otherwise known to those skilled in the relevant art. Recombinant cloning vectors will often include one or more replication systems for cloning or expression, one or more markers for selection in the host, e.g. antibiotic resistance, and one or more expression cassettes.

[0059] The terms “mutant” and “mutation” mean any detectable change in genetic material, e.g. DNA, or any process, mechanism, or result of such a change. This includes gene mutations, in which the structure (e.g. DNA sequence) of a gene is altered, any gene or DNA arising from any mutation process, and any expression product (e.g. protein or enzyme) expressed by a modified gene or DNA sequence. The term “variant” may also be used to indicate a modified or altered gene, DNA sequence, enzyme, cell, etc., i.e., any kind of mutant.

[0060] The term “homologous”, in all its grammatical forms and spelling variations, refers to the relationship between two proteins that possess a “common evolutionary origin”, including proteins from superfamilies (e.g., the immunoglobulin superfamily) in the same species of organism, as well as homologous proteins from different species of organism (for example, myosin light chain polypeptide, etc.; see, Reeck et al., Cell 1987;50:667). Such proteins (and their encoding nucleic acids) have sequence homology, as reflected by their sequence similarity, whether in terms of percent identity or by the presence of specific residues or motifs and conserved positions.

[0061] The term “heterologous” refers to a combination of elements not naturally occurring. For example, heterologous DNA refers to DNA not naturally located in the cell, or in a chromosomal site of the cell. Preferably, the heterologous DNA includes a gene foreign to the cell. A heterologous expression regulatory element is such an element operatively associated with a different gene than the one it is operatively associated with in nature. In the context of the present invention, an L1 gene is heterologous to the vector DNA in which it is inserted for cloning or expression, and it is heterologous to a host cell containing such a vector, in which it is expressed, e.g., a HUVEC cell.

[0062] The term “sequence similarity”, in all its grammatical forms, refers to the degree of identity or correspondence between nucleic acid or amino acid sequences that may or may not share a common evolutionary origin (see, Reeck et al., supra). However, in common usage and in the instant application, the term “homologous”, when modified with an adverb such as “highly”, may refer to sequence similarity and may or may not relate to a common evolutionary origin.

[0063] In specific embodiments, two nucleic acid sequences are “substantially homologous” or “substantially similar” when at least about 80%, and more preferably at least about 90%, at least about 95%, or at least about 99% of the nucleotides match over a defined length of the nucleic acid sequences, as determined by a sequence comparison algorithm known such as BLAST, FASTA, DNA Strider, CLUSTAL, etc. Sequences that are substantially homologous may also be identified by hybridization, e.g., in a Southern hybridization experiment under, e.g., stringent conditions as defined for that particular system.

[0064] Similarly, in particular embodiments of the invention, two amino acid sequences are “substantially homologous” or “substantially similar” when greater than about 80%, about 90%, about 95% or about 99% of the amino acid residues are identical or similar (i.e., are functionally identical). Preferably the similar or homologous polypeptide sequences are identified by alignment using, for example, the GCG (Genetics Computer Group, Program Manual for the GCG Package, Version 7, Madison Wis.) pileup program, or using any of the programs and algorithms described above (e.g., BLAST, FASTA, CLUSTAL, etc.).

[0065] A nucleic acid molecule is “hybridizable” to another nucleic acid molecule, such as a cDNA, genomic DNA, or RNA, when a single stranded form of the nucleic acid molecule can anneal to the other nucleic acid molecule under the appropriate conditions of temperature and solution ionic strength (see Sambrook et al., supra). The conditions of temperature and ionic strength determine the “stringency” of the hybridization. For preliminary screening for homologous nucleic acids, low stringency hybridization conditions, corresponding to a Tm (melting temperature) of 55° C., can be used, e.g., 5× SSC, 0.1% SDS, 0.25% milk, and no formamide; or 30% formamide, 5× SSC, 0.5% SDS. Moderate stringency hybridization conditions correspond to a higher Tm, e.g., 40% formamide, with 5× or 6× SSC. High stringency hybridization conditions correspond to the highest Tm, e.g., 50% formamide, 5× or 6× SSC. SSC is a 0.15M NaCl, 0.015M Na-citrate. Hybridization requires that the two nucleic acids contain complementary sequences, although depending on the stringency of the hybridization, mismatches between bases are possible. The appropriate stringency for hybridizing nucleic acids depends on the length of the nucleic acids and the degree of complementation, variables well known in the art. The greater the degree of similarity or homology between two nucleotide sequences, the greater the value of T_(m) for hybrids of nucleic acids having those sequences. The relative stability (corresponding to higher T_(m)) of nucleic acid hybridizations decreases in the following order: RNA:RNA, DNA:RNA, DNA:DNA. For hybrids of greater than 100 nucleotides in length, equations for calculating Tm have been derived (see Sambrook et al., supra, 9.50-9.51). For hybridization with shorter nucleic acids, i.e., oligonucleotides, the position of mismatches becomes more important, and the length of the oligonucleotide determines its specificity (see Sambrook et al., supra, 11.7-11.8). A minimum length for a hybridizable nucleic acid is at least about 10 nucleotides; preferably at least about 15 nucleotides; and more preferably the length is at least about 20 nucleotides.

[0066] In a specific embodiment, the term “standard hybridization conditions” refers to a T_(m) of 55° C., and utilizes conditions as set forth above. In a preferred embodiment, the Tm is 60× C; in a more preferred embodiment, the T_(m) is 65° C. In a specific embodiment, “high stringency” refers to hybridization and/or washing conditions at 68° C. in 0.2× SSC, at 42° C. in 50% formamide, 4× SSC, or under conditions that afford levels of hybridization equivalent to those observed under either of these two conditions.

Complex Diseases

[0067] In both the systemic and organ targeted autoimmune diseases, as well as other complex diseases such as schizophrenia and Alzheimer disease, there is good evidence for a genetic component. In some cases, familial forms of the disease have led to identification of altered genes that are also important in sporadic forms of the disease. For example, Alzheimer disease, the most common form of dementia, can be inherited as an autosomal dominant trait in some families. A study of four large kindreds first demonstrated linkage of early onset-Alzheimer disease with DNA markers on chromosome 21 (87). A number of subsequent studies have localized the AD1 locus to the site of the amyloid precursor protein (APP) gene on chromosome 21q. A review of multiplex Alzheimer pedigrees indicated that the APP locus accounted for 63±11% of those pedigrees, although only a subset of those families have mutations in the APP protein (88). While other genes, including presenilin-2 and apolipoprotein E, have also been associated with Alzheimer disease, it has been suggested by Hardy that the common feature of the many forms of Alzheimer disease is that they all involve altered processing of APP (89).

[0068] Genetic factors have been proposed to be involved in the etiology of schizophrenia for many years, but it is only with the advent of the use of microsatellite markers for genome analysis that regions of the genome that might be associated with the disease have been identified. As in Alzheimer disease, large extended pedigrees of families enriched for schizophrenia have been studied to more convincingly identify disease loci. A recent study of thirteen large families using 365 microsatellite markers identified five distinct loci with LOD scores>3.0 in the entire sample or in individual pedigrees (84). None of the specific genes within these loci have been determined.

[0069] In the case of autoimmune disorders the diseases can also run in families, and interestingly, some families have members with various autoimmune diseases. For example, a family might have one individual with SLE, another with IDDM, and another with an autoimmune thyroid disease. Genome studies have defined multiple loci that seem to be statistically associated with a diagnosis of one or another of these diseases. Some of these loci seem to be in common to multiple autoimmune diseases (9). There is the concept of “autoimmunity genes” and the idea of threshold. In contrast to single gene diseases, such as cystic fibrosis or sickle cell anemia, where there is one particular mutation or any of a number of alterations in one specific gene, in both systemic and organ targeted autoimmune diseases there is not one locus that is identified as linked to the disease. Rather there are many loci that seem to have a low level association. The current idea of threshold suggests that these loci represent sites of individual variability (not necessarily abnormality) in multiple genes that confer either altered levels of expression or subtly different quality or quantity of function, such that if an individual has a few of the variants that may confer disease susceptibility, they are unlikely to get the autoimmune disease. Comparatively, if they have several gene variants that confer susceptibility, their immune/inflammatory function would be such that they are more likely to develop the autoimmune disease. It has been proposed by others that most of the autoimmune disease susceptibility loci encode genes that are associated with the immune or inflammatory systems (e.g., IL-2, FcR, MHC molecules, cytokines, apoptosis molecules).

[0070] Pathogenic agents of autoimmune diseases such as, but not limited, to demethylating drugs and ultraviolet light, have been well studied and evaluated. Several autoantigens and autoantibodies have been characterized, the cytokines that are increased or decreased are known, and the roles of complement activation products and immune complexes have been studied. The mechanism or mechanisms that underlie the etiology of these disease states, however, is currently unclear. Despite the fact that some pathogenic agents are known, the mechanism of action of these agents (e.g. the mechanism by which these agents induce activation of an immune response to self antigens) is not known. Additionally, it is not understood why the targeted antigens in SLE tend to be components of particles that contain both proteins and nucleic acids or how the immune system becomes activated by these particles.

[0071] The term “breaking tolerance”, as used herein, is used to address the question as to what triggers an immune response to the relevant autoantigens in each of these disease states. During development, thymocytes that have high affinity for self antigens are removed from the system, and peripheral tolerance mechanisms operate in the mature immune system to discourage activation of self reactivity. T cells specific for some self antigens have probably not been efficiently deleted, but those antigens are likely to be those that are hidden away in “immune privileged” sites, such as the eye and testis, and for that reason an immune response is never generated.

[0072] There are features of DNA and RNA that can promote or induce immune responses. The CpG motifs (pairs of C's and G's) are particularly enriched in viral and bacterial DNA and can activate NF-kB and generally act as immune adjuvants. When these motifs are present in mammalian DNA they are usually methylated, resulting in “hiding” the DNA. The effect of the methylation would be to inhibit those motifs that can act as immune adjuvants and, should those motifs be present in a regulatory region of a gene, to inhibit their participation in transcriptional activation. RNA also can activate adjuvant activity that promotes immune system activation. Double stranded RNA can, through somewhat unclear mechanisms, induce the production of interferon-α, which in turn can promote dendritic cell (i.e., antigen presenting cell) function. Either of these events can provide sufficient immune stimulation to inappropriately trigger an immune response.

[0073] Another consideration in mechanisms of breaking tolerance is exposure of “cryptic” or altered epitopes. When an antigen or self antigen is processed by an antigen presenting cell, there are characteristic sites of protein cleavage that generate peptides expressed on major histocompatability class molecules to T cells. In the case of self antigens, self peptides are probably presented to thymocytes during development and those with high affinity for that peptide are removed from the system. If the self protein is then presented to T cells in an alternate situation (e.g., in association with another protein), or if the self-antigen is handled in a different manner in the antigen presenting cell, resulting in presentation of a different or altered epitope, the T cell component of the immune response may recognize that antigen.

[0074] Possible mechanisms through which self tolerance could be broken would include association of self-antigen with an effective adjuvant, such as DNA enriched in CpG motifs or RNA that can induce interferon-α, or presentation of a self antigen that looks different to the immune system, either because an atypical peptide is presented or because a typical peptide is presented in a different manner or context (like in association with an epitope from an immunogenic peptide). Finally, antigen dose may be important. If the immune system experienced sustained or recurrent exposure of a self-antigen, probably in the presence of an adjuvant activity, that self-antigen may be reacted to.

[0075] When proteins, whether foreign to the organism or self-proteins, interact with the immune system of an organism in the absence of pro-inflammatory stimuli, that immune system often fails to respond. In contrast, when a protein is introduced to the immune system together with a substance that triggers inflammation, an “adjuvant”, the properties of antigen presenting cells are altered to facilitate antigen-specific activation of T-cells. In the case of intracellular self-antigens, it is proposed that when the protein components of intranuclear or intracellular particles are associated with non-specific immune stimulants, an antigen-specific immune response to the protein components of that particle might be triggered. Those non-specific immune stimulants, or endogenous adjuvants, might include DNA enriched in CpG motifs, known to trigger activation of the pro-inflammatory transcription factor NF-kB, a protein that mediates tumor necrosis factor (TNFα) transcription, or RNA that can achieve a double stranded conformation and acquire the capacity to induce production of interferon-α. These are all known to induce the maturation and increase the antigen presenting capacity of dendritic cells.

[0076] Another concept that has been discussed in the context of “breaking tolerance” is the concept of “altered self”. The idea here is that a self-antigen might appear foreign to the immune system if it achieved a different amino acid sequence or conformation than its typical sequence or structure, if an antigen presenting cell processed the protein in an atypical manner, or if the peptide generated by the antigen presenting cell bound to the groove of MHC molecules in an atypical orientation. Somatic mutations in genes such as p53 are known to induce an immune response to the altered p53. Chromosomal translocations can generate fusion proteins of two genes. Activation of caspases in the setting of apoptosis generates cleavage products of self-proteins that might be capable of immune system activation.

Systemic Autoimmune Diseases

[0077] Prototypical systemic autoimmune diseases include SLE, scleroderma, mixed connective tissue disease, Sjögren's syndrome and other systemic disorders. Epidemiological studies indicate that typical onset of these diseases occurs in the teenage years to the 20's (i.e., post-puberty). Additionally, studies indicate that these diseases affect women in significantly greater numbers than men, in a ratio of about 8-9:1.

[0078] These disease states are characterized by generalized immune system activation, but with evidence for antigen-specific induction of T cell-dependent autoantibodies. For example, in SLE autoantibody specificities are very characteristic. Autoantigens include nucleosomes (particles containing histones and DNA); ribonucleoprotein (RNP) particles (containing RNA and proteins that mediate specialized functions in the RNP particle); and double stranded DNA. An example of proteins that mediate specialized functions in the RNP particles is the Sm protein, which has spliceosome function. It is theorized that an inappropriate immune response is initiated in SLE. The response appears to be initiated to a component of one of the intracellular particles, which then spreads to other components of the particle. Tissue damage mostly occurs through actions of the autoantibodies, including activation of the complement system, although antigen-specific T cells also may play a direct role in tissue damage. The tissue damage may be triggered or exacerbated by drugs that may demethylate DNA and by sunlight (e.g. UV light).

Organ Targeted Autoimmune Diseases

[0079] Organ targeted autoimmune diseases include IDDM, MS, autoimmune thyroid disease, RA, pemphigus, psoriasis, polymyositis, dermatomyositis, pemphigoid, vitiligo, primary bilary cirrhosis, chronic active hepatitis, Crohn's disease, ulcerative colitis and pernicious anemia. Epidemiological studies indicate that these diseases have variable onset. Gender distribution studies indicate that some are more common in females, whereas others have a more even gender distribution.

[0080] These disease states are characterized by an inappropriate immune response to a self-protein. The response often spreads to include other antigens, notably those that are enriched in the target organ. For example in IDDM, the earliest detectable immune response is directed at the protein glutamic acid decarboxylase (GAD) with later responses directed toward insulin. The self-proteins targeted in some other organ-specific autoimmune diseases include, desmoglein 3 in pemphigus vulgaris, desmoglein 1 in pemphigus foliaceus, myelin oligodendrocyte glycoprotein in MS, tyrosinase related protein in vitiligo, thyroid stimulating hormone receptor in autoimmune thyroid disease, bullous pemphigoid antigen 1 in bullous pemphigoid, and SP100 in primary bilary cirrhosis. There are other complex autoimmune diseases, rheumatoid arthritis for example, in which the relevant autoantigens have not been identified. Antigen-specific T-cells triggered by these antigens mediate tissue damage in the target organ. Cytokines and autoantibodies also may contribute to development of the disease state.

Long Interspersed Nuclear Elements (LINEs)

[0081] As described above, LINEs are believed to be fragments of a nucleotide sequence that has been distributed at many locations throughout the genome, and contain a 5′ regulatory region and two open reading frames (ORF) that can encode two proteins (ORF1 and ORF2). These two ORFs are transcribed into mRNA, which are copied back (or parts of it) into DNA, and the DNA inserted back into the genome.

[0082] Thus, a key role for LINEs in driving the increasing sophistication and diversity of the immune system throughout evolution is supported by the heavy load of those elements in the segments of the genome encoding the major histocompatibility (MHC) complex, immunoglobulin heavy and light chains, and T cell receptors. In addition, L1 elements may have been important in the evolution of genomes in general, by generating diverse genomic substrates of sequence modules, along with mutations superimposed on those modules, that could be selected, or not selected, for improved function at the molecule, cell, or organism level. Such a function would justify the maintenance of these potentially damaging genetic elements: they continually build the integrity of the host defense system and the effective function of the organism. Genes that jump into various places in the genome could significantly alter the function of various proteins. Therefore, it is believed that few of these LINEs are capable of doing so. It has been estimated that of the more than 100,000 LINE sequences, there are only approximately 30-60 functional (able to transpose) LINEs in the diploid human genome (43,59). Tight regulatory control of those potentially active elements is likely.

Expression and Function of L1 Products

[0083] In studies on the tissue and cell expression of the products of L1 elements, L1 products have been observed in both germ cells and non-germ cells of testis and ovary, in syncytiotrophoblast cells of the placenta, as well as in breast carcinoma cells (56, 57, 67). The best-studied systems are several teratocarcinoma cell lines, which have been used to define the compartmentalization of the ORF1 p40 in cytoplasmic RNP particles (54). The testis is a fairly well-protected immune privileged site, and germ cells are constantly generated without stimulation of the male immune system. Comparatively, the ovary is more accessible to the immune system, and its products, the ova and shed follicular cells, may be found in various areas within the body such as, but not limited to, the peritoneal cavity. Additionally, eggs are generated episodically, a kinetic pattern which is proposed to be more conducive to immune system triggering (e.g., priming, followed be monthly boosting). While the immune system is somewhat suppressed during pregnancy, if L1 proteins are expressed in the placenta, there might be the opportunity for some immune reactivity to them. The placenta is a target of disease in some lupus patients. Thus, these proteins can play a role in generating diversity in the germ cell, as a supplementary mechanism to crossing-over/recombination. Sex-specific differences in the regulation of L1 gene regulation, as by SOX family proteins for example, may modulate their expression in males more than females. In addition, their limited distribution may mean that they are not so available to effectively induce immune tolerance and if present in sufficiently high levels post puberty, may be able to trigger an immune response to themselves or their associated proteins or nucleic acid. Expressed sequence tags (ESTs) from normal breast tissue also encode ORF1 p40.

[0084] Beyond this location of L1 proteins in reproductive organs, there have been a few reports, mostly in mouse literature, showing L1 products in lymphocytes (55). B cells can act as antigen presenting cells when activated, so the B cell could be both a source of L1-derived self antigens as well as the cells that present those antigens to T cells, thus initiating an autoreactive immune process. One paper has suggested that L1-containing particles (proteins and nucleic acid) can assist in repairing double stranded DNA breaks (60). Of the 3 unique processes that B cells undergo, VDJ recombination, Ig class switching, and somatic hypermutation, all three require cleavage of double stranded DNA. Without wishing to be bound by theory, it is possible that the protein products of L1 elements might be recruited to perform a physiologic function that is DNA repair related. Interestingly, the classic autoantigen targeted by autoantibodies in SLE is a double stranded DNA. If the immune system were exposed to double stranded DNA in association with L1 proteins, to which the immune system is not tolerant, along with the adjuvant activities (such as interferon-″) induced by the presence of L1 RNA, the double stranded DNA may be targeted by the immune response. L1 products may also be present at sites of inflammation. Expression of L1 ORF1 p40 mRNA and protein has been observed in RA synovial tissue and has been suggested to have the capacity to trigger intracellular kinase pathways that mediate inflammation (58).

[0085] Several of these elements have been roughly localized on their appropriate chromosomes. Some of the L1 elements are polymorphic (43,44). Interestingly, L1 sites of a small population study showed that the African American ethnic group had the highest frequency of a particular L1 element located to chromosome Iq (43). Beyond individual variability in the presence or absence of a given L1 element, the 5′ regulatory region as well as the 5′ part of ORF1 are quite variable. So there might be base differences from one individual to another that would affect the efficiency of transcription or function of the encoded protein product of ORF1 (the p40 protein).

[0086] While the human 5′ regulatory region of the gene encoding ORF1 is a single stretch of nearly 900 bp, the mouse 5′ regulatory region comprises variable numbers of tandem repeats of a CpG island, along with a short tether that anchors the modules to the ORF1 coding sequence. The 5′ 40% of mouse and human ORF1 sequences are unrelated. Although this application focuses on human diseases, disease genes, and susceptibility and triggers for human disease, it is predicted that murine L1 elements will be found near murine susceptibility loci as preliminarily found in human chromosomes.

[0087] L1 proteins are usually not expressed. Therefore, there must be reasonably effective controls in place that inhibit transcription of L1's. One potential mechanism is the methylation of CpG motifs in the 5′ regulatory region. There are studies indicating the importance of these motifs in regulation of L1 expression. It is of interest that many of the drugs that typically induce lupus have the effect of demethylating DNA. Moreover, a murine model of lupus has been established in which treatment of mouse lymphocytes with 5-azacytidine can result in the capacity of those lymphocytes to induce lupus. Similarly, there are suggestions that UV light, a classic exogenous trigger of disease exacerbation in SLE, can promote gene transcription of L1 elements. In addition, the inhibitory capacity of the SRY male-specific transcription factor in regulation of L1 transcription suggests that L1 may be more stringently regulated in males compared to females.

Role of L1 Retrotransposons in Human Disease

[0088] Documentation of functional activity of L1 elements has been provided by instances of gene inactivation following insertion of a retrotransposon (61-64). Such genetic diseases have been documented in man, mice, and dogs (36). Among the first and best studied germline insertions are those into the factor VIII and dystrophin genes of individuals with sporadic (i.e., no family history) hemophilia and muscular dystropy, respectively (61,64). Therefore, it was proposed that the L1 transposed into the previously normal gene, disrupting its expression. Kazazian described insertion of the 3′ end of L1 into exon 14 of the factor VIII gene in two unrelated patients with hemophilia (61).The limitation of the transposed element to its 3′ portion is typical; it is a rare L1 sequence in which the 5′ segment is not truncated. The Fas mutation that accounts for the lupus accelerating phenotype in MRL/lpr mice represents an insertion of a retrotransposon into that gene (65). These rare instances of gene disruption are striking but may not represent the most significant impact of L1 elements in human disease. Some instances of chromosomal translocation in malignancy are associated with insertion of a partial or full-length L1 element into one of the transposed gene partners.

[0089] Most relevant to the pathogenesis of complex diseases, particularly autoimmune diseases, transcriptionally active L1 elements may provide the trigger for disease initiation. At least eight mechanisms can be postulated through which retrotransposons could mediate human disease: 1) gene disruption; 2) gene transposition; 3) induction of mutations in nearby genes; 4) altered transcriptional regulation of a gene by a nearby L1 element; 5) altered splicing or translation of a mRNA based on inclusion of L1 elements in its intronic or untranslated segments; 6) induction of an immune response to the transcribed and translated products of the retrotransposon; 7) induction of an immune response to co-transcribed genes adjacent to a retrotransposon; 8) induction of an immune response to proteins, DNA, or RNA physically associated with L1 DNA, RNA or protein.

[0090] The present invention discloses that the complex pattern of multiple SLE genetic susceptibility loci identified in microsatellite total genome studies can represent replicate copies of one family of genes, the L1 retrotransposon elements, rather than many discrete genes. This model can also apply to other systemic autoimmune diseases, as well as complex diseases not known to be autoimmune in nature. While polymorphisms in individual genes that regulate immune system activity or tissue response may play a role in disease expression, the bulk of SLE genetic susceptibility can be attributable to variable expression or efficiency of transcription of members of the L1 element family. The RNA and protein products of those L1 elements would act in a threshold manner to trigger immune reactivity to intracellular RNP particles, co-transcribed gene products, and possibly to double stranded DNA breaks, RNA, or proteins to which L1 products bind. The present invention further identifies potential therapeutic targets.

[0091] L1 retrotransposon elements or their products can be the primary triggers of the antigen-specific immune system activation that results in the inflammatory and tissue destructive manifestations of complex diseases such as SLE. Although the individual whose genome is enriched in full-length L1 elements capable of retrotransposition will be particularly susceptible to these diseases, successful transposition would not be a requirement for disease induction. If the L1 coding region is transcribed into mRNA and that RNA into ORF1 p40 protein, those events might be sufficient to trigger complex disease, the prototype being SLE. The presence of the specific L1 RNA, with sequence features common to RNA viruses, along with the p40 protein in cytoplasmic RNP particles, also might trigger autoimmunity through a compound mechanism. Expression of p40 is highly restricted in both time and location (56,57). In view of this limited expression, central immune tolerance to p40 might be only partial, resulting in an immune system ready for activation should antigen load pass a threshold. The presence in a particle of RNA with the sequence features of viral RNA might stimulate cellular production of interferon-α, a cytokine that provides a mechanistic bridge between innate and adaptive immunity. The effect would be an immune system milieu supportive of an antigen-specific response to components of the RNP particle itself, as well as any associated proteins or nucleic acid fragments. The chronic and recurrent immune response stimulated in this way would result in the spectrum of pathogenic autoantibodies typical of SLE, as well as the secondary manifestations of immune system activation and dysfunction that are well described (69, 70). An additional method of induction of autoimmune disease by retrotransposons, described in the fourth mechanism above also may have some role in these diseases. Increased transcription of a gene may be mediated by effects of a nearby L1 element on the promoter region of the gene. The increased production of that gene product might be sufficient to cross a threshold for induction of an immune response under appropriate immunostimulatory conditions. Another related method of induction of autoimmune disease is described in the seventh mechanism described above. Transcriptionally competent L1 elements might activate an immune response to the products of nearby genes. Transcription of nearby genes can generate “readthrough” transcripts that include L1 sequences, and conversely, transcription of the LINEs may activate or modulate transcription of genes 3′ to the L1 element. In either case, the presence of L1 nucleotide sequences and p40 protein together with a normal gene product might trigger immune reactivity to that gene product.

[0092] Those individuals with highly active L1's that encode ORF1 and ORF2 proteins with perfect or near-perfect sequence (meaning the proteins translated will function effectively) may be susceptible to random insertions into genes, disrupting the function of those genes and causing significant disease mediated by impaired function of a single gene (as in the noted case of hemophilia). Such individuals may also be more likely than others to produce L1 RNA transcripts, along with ORF1 and/or 2 proteins, that cluster together, along with other proteins, in RNP particles in whatever cells are most likely to make these products, such as ova and follicular cells in the ovary, cells in the testis, placental trophoblast cells, breast tissues, and possibly B (and/or T) lymphocytes. The RNA, known to fold into 3-dimensional conformations, and with sequence features with some similarities to viral RNA, may trigger production of interferon, an immunostimulant. The DNA copied from that RNA will be rich in CpG motifs with adjuvant properties. If the immune system (CD4+ T cells) becomes exposed to these L1 proteins, L1 RNAs, and/or L1 DNAs, along with the adjuvant factors (interferon, etc.), “breaking tolerance” and triggering an immune response to any or all of the components of those particles will be set up. The immune response is known to undergo determinant spreading from an initial triggering epitope in a particulate antigen to other epitopes. As such a response developed, a spectrum of autoantibodies would emerge that are characteristic of those seen in patients with SLE. This autoantibody response might also include some directed toward double stranded DNA, targeted because it associates with the L1 products at sites of DNA cleavage, or proteins. Those individuals with genetic susceptibility to SLE would correspond to those individuals with either more L1 elements in their genome and/or more functional (transcribable) L1 elements. Those individuals could be identified by generating a map of the location of high fidelity (with DNA sequence very similar to or identical to the characterized active L1 elements), full-length (able to encode ORF1 and/or ORF2 protein) L1 elements, sequencing the DNA of an individual in those regions of the genome, and determining the presence of the elements, their fidelity to consensus, and whether they are full-length (with full regulatory region, ORF1 and ORF2). The location of such L1 elements on chromosome 1q and 16 are proximate to several of the markers that have been identified for lupus susceptibility loci. Individuals with L1 elements that are located in intronic segments of genes would also be identified by mapping such elements and the genes they are associated with and then sequencing or otherwise characterizing the DNA of the individual. The sequencing and DNA analysis can be performed using any method known in the art such as, polymerase chain reaction, SSCP, or Southern blotting.

[0093] Additionally, L1 elements which confer susceptibility may be those L1 genes situated near genes such that they either confer increased transcription immunogenicity on the nearby gene or confer increased immunogenicity on the nearby gene product. If an L1 element is sufficiently intact to initiate gene transcription, but not of sufficient fidelity to the consensus sequence to produce functional ORF1 and 2 proteins, it might produce a transcript that is a hybrid of the L1 transcript and the neighboring gene transcript. If the host gene mRNA is translated into protein and remains associated with the L1 transcript, tolerance to the gene product might be broken by virtue of the induction of adjuvant activity by the L1 transcript. Or conversely, activation of the host gene in the normal physiologic course of events, or in the setting of infection or stress, would result in transcription of L1 mRNA, along with the host gene mRNA. Either way, host gene products would be physically associated with potentially immunogenic L1 products. From the data obtained, these L1's are of fairly high fidelity (usually 85 to 9596%), but probably not sufficiently high fidelity to represent a fully active element. This level of sequence fidelity may reflect competence sufficient to initiate transcription but not to produce functional proteins. These locations can be mapped and individual DNA samples tested to determine the presence and the degree of fidelity and intactness of these L1 sequences.

Methods for Identifying Genes and Gene Products Involved in Human Disease

[0094] The present invention provides a method that allows the identification of genes and gene products that are candidates for involvement in human disease. In view of the important role that L1 elements have likely played in the evolution of the human and other genomes, and the requirement that a functional L1 element be full-length (including the entire or nearly entire approximately 6000 nucleotide sequence) and capable of being transcribed and translated into functional protein, the identification in the genome of the location of full-length L1 elements of high level identity to the consensus sequence of a known functional L1 element can be used to identify genes relevant to human disease. Moreover, the identification of genes or mRNAs in which full-length L1 elements are included in intronic or untranslated segments can be used to predict candidate disease genes, mRNAs, and proteins important in human disease. This invention is based on the hypothesis that individual genomic variability can be reflected in disease, and the location of L1 elements can provide an important predictor of the sites of disease-relevant genomic variability.

[0095] In general, the method can be exercised by cataloguing the location of L1 elements in the genome, without prior information regarding disease susceptibility loci, or it may be exercised by studying a segment of the genome in a region encompassing that locus. The method in either case involves the comparison of the sequence of a known segment of DNA with the DNA sequence of the 5′ segment of a known functional L1 element. The known segment of DNA may be derived from a contig, a bacterial artificial chromosome (BAC), or a gene sequence published in a publicly available database or any proprietary DNA sequence of more limited availability. In some cases, RNA sequences may also be useful for analysis.

[0096] The L1 sequence used for comparison can be derived from a publicly available sequence of a full-length L1 element that has been demonstrated to be capable of transposition. As the genome is composed of thousands of fragments of L1 elements derived from the 3′ end of the consensus sequence, it is cumbersome to conduct comparison searches of the entire L1 sequence with a test genome sequence. The method is therefore most effectively conducted by use of the 5′ region of a consensus L1 element.

[0097] For example, in Examples 8-11 herein, approximately the most 5′ 900 nucleotides of the L1 sequence located on chromosome 1q, termed LRE2 and published in the GenBank database under accession number U09116, was used in Pairwise BLAST “BLAST 2 sequences” searches against one or a series of contigs, BAC clones, or any published DNA sequence. The method is also effective using shorter segments of the 5′ region of the consensus L1 sequence. The important aspect of the method is that the most 5′ segment of the sequence, whether it be the most 5′ 100 or the most 5′ 900 bases, is used.

[0098] Matches with the test DNA segment are scored as positive if they meet either of three criteria: 1) the tested DNA sequence has about 97%, about 98%, about 99%, or about 100% identity to the 5′ region of the consensus L1 element, specifically nt 1-884 of U09116. and is located within about 200,000, more preferably about 100,000 bases, and even more preferably about 50,000 bases of a gene2) the tested DNA sequence includes the 5′ region of a consensus L1 element in an intron or untranslated segment. 3) In addition, full-length high fidelity are scored positive if their 5′ sequence is about 98%, about 99%, or about 100% identical to nt 1-883 of the L1 consensus sequence from U091 16, even if they are not located in close proximity to a gene or predicted gene. For the first criteria, the selection of 100,000 bases for the margins of proximity of the high fidelity L1 element to a neighboring gene is assigned arbitrarily based on studies indicating that gene regulation can be modified by sequences as distant as 100,000 bases, but these criteria to do not strictly limit the method to that DNA distance (90). The distance between the first nucleotide of the L1 element and the first nucleotide of the susceptibility gene can be measured as bp. A potential disease gene is identified as being less than about 200,000 bp, preferably less than 100,00 bp, and most preferably less than about 50,000 bp from the 5′ end of the L1 element. The second criterion does not require that the L1 sequence must be of 98, 99, or 100% sequence identity to the consensus L1 sequence. Typically, full-length L1 elements included in intronic gene segments range from 80-99% fidelity to the consensus L1 sequence. It should be noted that occasionally L1 sequences do not extend to the very 5′ extent of the consensus sequence, but may rarely lack up to the most 5′ 10 bases.

[0099] Once a list of genes proximal to a full length high fidelity L1 element, or containing an L1 element in their intronic regions, has been generated, those genes can then be further explored for a role in disease pathogenesis, as sites of individual variability that confers susceptibility to disease, as participants in disease-relevant molecular pathways, and as potential targets for therapy.

Methods for Determining Disease Susceptibility or Diagnosing a Complex Disease

[0100] In order to determine susceptibility to, or diagnose, a complex disease in an individual, the presence on a particular chromosome in an individual's genome of an L1 element that is capable of being transcribed can be assessed. The presence of an intact 5′ regulatory region in the context of the adjacent DNA sequence specific to that chromosomal location can be determined. Some L1 elements will either be present or absent. Additionally, some L1 elements may be present but contain variable nucleotides (nt) in different individuals.

[0101] PCR and nested PCR techniques may be used to amplify sequences of interest. Nested primer sets for PCR are designed using the nucleotide sequence that includes approximately 800 nt 5′ of the initiation of the 5′ regulatory region of the L1 element and the first approximately 50 nt in the L1 regulatory region. DNA can be isolated from a variety of sources including, but not limited to, peripheral blood cells or another cell source, from a patient with an autoimmune or complex disease or who may be suspected to be susceptible to or possibly developing an autoimmune or complex disease. The presence of a PCR amplified product can then be associated with the presence or absence of an autoimmune disease in a population of patients, or in a subpopulation of patients expressing particular clinical or laboratory features of the disease, and compared to the presence of a similar band in control subjects. The same method may also be used to study individuals suspected to be susceptible to or possibly developing a complex disease that is not traditionally considered an autoimmune disease. Examples of such diseases are Alzheimer disease and schizophrenia, but the method is not limited to those diseases.

[0102] The presence or absence of an L1 element containing an intact 5′ regulatory segment at a particular chromosomal site also can be determined with Southern blot analysis under conditions of high stringency using well known techniques. As in the case of PCR and nested PCR, the presence of the 5′ regulatory region of the L1 element of interest can be determined by the presence of a band indicating reaction of the labeled probe with the particular DNA segment of interest.

[0103] In some cases the presence or absence of the 5′ regulatory region of the L1 element will be observed, in other cases, the 5′ regulatory element will be present, but it will have nt variations in the study individual compared with DNA from healthy or disease control individuals. These nt variations can be detected by direct sequencing of the products of either the initial PCR reaction described above, or the nested PCR reaction. The PCR product can either be directly sequenced, using an automated sequencing instrument, or the PCR product can be subcloned into a cloning vector, positive clones picked, plasmid DNA prepared and directly sequenced. Alternative approaches to mutation detection can also be used to identify individual differences in nt sequences in the amplified PCR product. The presence or absence of nucleotide changes at a particular site in the 5′ regulatory region can be studied for association with a diagnosis of autoimmune or other complex disease, or clinical or laboratory features of the disease.

[0104] Once it has been determined that an individual contains in their genome an L1 element that is located at a particular chromosomal location, the presence in that L1 element of a full-length 5′ regulatory region and the approximately 5′ one third of ORF1 that are of high fidelity to a consensus L1 sequence can be determined using a 5′ primer and a 3′ primer that is located at the approximate mid-point of ORF 1. The PCR product can be directly sequenced or subcloned and sequenced as described above. The presence of an L1 element at the particular chromosomal location that is full-length and/or is of high fidelity compared to a consensus sequence can be determined using DNA isolated from cells or tissue of an individual with or suspected to have or to be susceptible to an autoimmune disease, and compared to DNA from a healthy or control individual. Other approaches can be taken to identify individual nt differences in these regions between and among DNA from different individuals. For example, high pressure liquid chromatography can be used to determine heteroduplex formation between two strands of DNA spanning the 5′ regulatory region and 5′ segment of ORF1 of an L1 element located at a particular chromosomal site in order to identify nt differences between the DNA strands of two individuals.

[0105] The presence of an L1 element within the regulatory region or in an intron of a gene can modify the expression of that gene. If that gene product is important in the immune or inflammatory pathways, altered expression of the gene product can contribute to autoimmune disease. Alternatively or additionally, the presence of an L1 element in a location proximate to a gene or within the introns of a gene may result in generation of an RNA product that includes RNA sequences encoded by the L1 element as well as RNA sequences encoded by the neighboring or surrounding gene. Such an RNA transcript may promote an autoimmune reaction to the product of the neighboring or the surrounding gene. The presence of an L1 element within or near a gene can be determined by identifying the location of that gene of interest, identifying a DNA sequence in the Genbank that includes an L1 sequence within or proximate to the gene of interest, and identifying PCR primers that will amplify a segment of that L1 element in the context of the chromosomal site in which it is located. DNA from an individual can then be assessed for the presence of that L1 element, or for the particular sequence of that L1 element, using PCR or nested PCR, Southern blots, direct sequencing, or other techniques.

[0106] The presence of an insertion in an individual with an autoimmune disease, or one who is suspected to be susceptible to developing an autoimmune disease, can be detected by isolating DNA from blood or tissue cells, or any other DNA source, from that individual and designing PCR primers that will amplify the L1 insertion in the context of the chromosomal locus of interest. The presence of a PCR amplified product can then be associated with the presence or absence of an autoimmune disease in a population of patients, or in a subpopulation of patients expressing particular clinical or laboratory features of the disease, and compared to the presence of a similar band in control subjects. Such an L1 element can also be identified using ³²P-labeled DNA probes in a Southern blot.

Transcription of L1 Elements

[0107] Transcriptional activity of L1 elements can be assessed by techniques that detect and quantitate mRNA encoded by the L1 element ORF1 or ORF2. Production of the protein products of L1 elements can be detected and quantified by techniques that identify a specific protein. Cells, tissues or body fluids (e.g., blood, serum, saliva, urine, tears, sweat, synovial fluid, cerebrospinal fluid and the like) can be isolated from an individual with an autoimmune disease or suspected to be susceptible to developing an autoimmune disease in order to measure L1 encoded mRNA or protein. In situ hybridization can also be used to detect the mRNAs encoded by L1 elements. In some cases, it may be desirable to induce the expression of L1 mRNA products by treating an individual's cell sample, such as peripheral blood mononuclear cells with an agent that stimulates the transcription of L1 mRNA, including but not limited to 5-azacytidine.

[0108] Detection of the protein products of L1 elements, either ORF1 or ORF2 gene products, can be used to indicate the presence in cells, tissue, or body fluids of potential immune system triggers that can induce or exacerbate autoimmune disease. Proteins can be detected by several techniques well known to those of ordinary skill in the art, including immunoprecipitation or Western blot using polyclonal or monoclonal antibodies to the ORF1 or ORF2 products, immunofluorescence or flow cytometry to detect intracellular or cell surface expression of these proteins, immunohistochemistry to detect the proteins in tissue samples, or ELISA to detect L1-encoded ORF1 or ORF1 proteins in plasma, serum, or other body fluids. In some cases, it may be desirable to isolate cells from an individual, as from peripheral blood, and treat those cells with a demethylating agent such as 5-azacytidine or an agent that promotes histone acetylation before isolation of proteins.

[0109] Characterization of the nucleotide and protein components of ribonucleoprotein particles can be performed to detect the presence of potentially immunostimulatory L1 products, L1 protein products that can serve as autoantigens, or gene sequences that are expressed in association with L1 products and the protein products of which might become immunogenic when expressed in association with those L1 products. Ribonucleoprotein particles can be isolated from cells derived from an individual suspected of having autoimmune disease (71). The presence of L1 mRNA components in the ribonucleoprotein particles can be detected by generating cDNA followed by PCR amplification using specific primers, or unknown mRNA sequences can be characterized by generating cDNA, followed by direct sequencing. Such RNA transcripts of unknown sequence within ribonucleoprotein particles may identify RNA sequences encoded by genes neighboring or surrounding L1 elements and their protein products may represent putative autoantigens.

[0110] Patients with autoimmune disease, particularly those with SLE, often make antibodies with specificity for nucleotide or protein components of intracellular particles, including ribonucleoprotein particles. The presence of autoantibodies specific for L1 DNA or mRNA sequences or for L1 protein products may indicate a diagnosis of autoimmune disease. Detection of a change in the titer or level of those antibodies may be associated with a change in the clinical disease activity of the patient. Serum autoantibodies specific for L1 products can be detected by the techniques of ELISA or immunoblot (72), or other newer techniques such as autoantigen-coupled beads or antigen microarray, with patient serum used to detect the L1 DNA, RNA or protein products, or by immunoprecipitation (72), in which the patient serum is used to precipitate cellular components containing L1 products, or purified L1 products.

Identifying L1-Elements—Experimental Techniques

[0111] This section describes various specific embodiments of the methods of the invention, and includes techniques for identifying L1 elements, their transcription products, and translation products. The L1 sequence found on chromosome 1q25, at approximately 184.5M bases from the 1p telomere, serves as an example of these approaches.

Identification of High Fidelity L1 Elements With Intact 5′ Regulatory Segments

[0112] A bacterial artificial chromosome (BAC) clone that includes the L1 element on chromosome 1q25, at approximately 184.5M bases from the 1p telomere, is identified by Genbank accession number AL162431. This sequence is also contained within the contig with GenBank accession number NT_(—)004552. Nested primer sets for PCR are designed using the nucleotide sequence that includes approximately 800 nt 5′ of the initiation of the 5′ regulatory region of the L1 element (the beginning of the L1 5′ regulatory region is considered to be located at nucleotide 14,948 in clone AL162431) and the first approximately 50 nucleotides in the L1 regulatory region (FIG. 1). For example, DNA can be isolated from peripheral blood cells, or another cell source, from a patient with an autoimmune disease, with a family member with an autoimmune disease, or who may be suspected to be susceptible to or possibly developing an autoimmune disease. DNA can also be isolated from blood or another source of cells from a healthy control individual or from an individual with a non-autoimmune disease. In this example, for BAC clone AL162431, a 5′ primer of sequence

[0113] CTG CCA TAC TGT ATA CCA GG (SEQ ID NO:5)

[0114] identifying a region of chromosome 1q that is 5′ of the L1 5′ regulatory region, and a 3′ primer of sequence

[0115] CTG TTC CTA TTC GGC CAT CT (SEQ ID NO:6)

[0116] identifying a segment of the L1 5′ regulatory region, can be used to amplify the DNA segment spanning nt 14,927 and 15,656. The PCR product is run on an agarose gel and the presence or absence of a band, representing the product of the PCR reaction, observed. The specificity of the PCR amplification can be further increased by performing a nested PCR reaction, in which the PCR product from the first reaction is excised from the gel, passed through a spin column to remove the first pair of primers, and the product then used as a template in a second PCR reaction that uses primers internal to the first set. For example, a 5′ internal primer of sequence

[0117] CTA GGG CCC AGA AAT ATA AG (SEQ ID NO:7)

[0118] and a 3′ internal primer of sequence

[0119] CCC CGG ATT ATT CTT ATT AC (SEQ ID NO:8)

[0120] can be used to amplify the first PCR product (FIG. 1). The resulting product, corresponding to nucleotides 15,619 to 14,946 of BAC clone AL162431, is run on an agarose gel, and the presence or absence of a product observed. The presence of a PCR amplified product can then be associated with the presence or absence of an autoimmune disease in a population of patients, or in a subpopulation of patients expressing particular clinical or laboratory features of the disease, and compared to the presence of a similar band in control subjects.

Southern Blot Analysis

[0121] Other techniques can be used to determine the presence or absence of an L1 element containing an intact 5′ regulatory segment at a particular chromosomal site. For example, the primers described above can be used to amplify the segment that includes the chromosomal region 5′ of the L1 as well as a portion of the 5′ regulatory region of the L1 element. This PCR product can be labeled with ³²P and used as a probe to determine the presence of the complementary DNA fragment in the genome of an individual. DNA is isolated from the individual, and run on an agarose gel after digestion with a restriction enzyme, and then the DNA probed with the ³²P-labeled DNA fragment. As in the case of PCR and nested PCR, the presence of the 5′ regulatory region of the L1 element of interest can be determined by the presence of a band indicating reaction of the labeled probe with the particular DNA segment of interest.

Direct Sequencing of PCR Products

[0122] While in some cases the presence or absence of the 5′ regulatory region of the L1 element will be observed, in other cases, the 5′ regulatory element will be present, but it will have nt variations in the study individual compared with DNA from healthy or disease control individuals. For example, the two BAC clones that identify a particular DNA region may contain nt variations. These nt variations can be detected by direct sequencing of the products of either the initial PCR reaction described above, or the nested PCR reaction. The PCR product can either be directly sequenced, using an automated sequencing instrument, or the PCR product can be subcloned into a cloning vector, positive clones picked, plasmid DNA prepared and directly sequenced. Alternative approaches to mutation detection can also be used to identify individual differences in nt sequences in the amplified PCR product. The presence or absence of nucleotide changes at a particular site in the 5′ regulatory region can be studied for association with a diagnosis of autoimmune disease, or clinical or laboratory features of the disease.

Sequencing of ORF1 L1 Elements Identified at Particular Chromosomal Locations

[0123] Once it has been determined that an individual contains in their genome an L1 element that is located at a particular chromosomal location, the presence in that L1 element of a full-length 5′ regulatory region and the approximately 5′ one third of ORF1 that are of high fidelity to a consensus L1 sequence can be determined using the 5′ primer described above (identifying the non-L1 chromosome-specific sequence) and a 3′ primer that is located at the approximate mid-point of ORF 1. The PCR product can be directly sequenced or subcloned and sequenced as described above. The presence of an L1 element at the particular chromosomal location that is full-length and/or is of high fidelity compared to a consensus sequence can be determined using DNA isolated from cells or tissue of an individual with or suspected to have or to be susceptible to an autoimmune disease, and compared to DNA from a healthy or control individual. Other approaches can be taken to identify individual nt differences in these regions between and among DNA from different individuals. For example, high pressure liquid chromatography can be used to determine heteroduplex formation between two strands of DNA spanning the 5′ regulatory region and 5′ segment of ORF1 of an L1 element located at a particular chromosomal site in order to identify nt differences between the DNA strands of two individuals (83).

L1 Elements as Disease Genes or Autoantigens

[0124] L1 elements inserted within or near genes may be implicated in the pathogenesis of an autoimmune disease or may themselves serve as autoantigens in an autoimmune disease. The presence of an L1 element within the regulatory region or in an intron of a gene may modify the expression of that gene. If that gene product is important in the immune or inflammatory pathways, altered expression of the gene product can contribute to autoimmune disease. Alternatively or additionally, the presence of an L1 element in a location proximate to or within a gene may result in generation of an RNA product that includes RNA sequences encoded by the L1 element as well as RNA sequences encoded by the neighboring or surrounding gene. Such an RNA transcript might promote an autoimmune reaction to the product of the neighboring or surrounding gene. Alternatively or additionally, the presence of an L1 element in or near the regulatory element of a nearby gene may alter the transcription of that gene, resulting in increased production of the gene product, and altered capacity to induce immune system activation. In addition, the presence of and L1 element in the intron or untranslated region of a gene may alter the splicing, mRNA stability, or translation of the mRNA or alter the folding or degradation of the encoded protein. The presence of an L1 element within or near a gene can be determined by identifying the location of that gene of interest, identifying a DNA sequence in the Genbank that includes an L1 sequence within or proximate to the gene of interest, and identifying PCR primers that will amplify a segment of that L1 element in the context of the chromosomal site in which it is located. DNA from an individual can then be assessed for the presence of that L1 element, or for the particular sequence of that L1 element, using PCR or nested PCR, Southern blots, direct sequencing, or other techniques.

[0125] For example, at least three BAC clones published in the Genbank include the DNA sequence of the region on chromosome 1q that encodes members of the family of receptors for the Fc segment of immunoglobulin (FcR), as well as several other genes including ATF6. BAC clone AL359541, located approximately 162.3M bases from ptel, contains an L1 insertion in an intron of the FcR/ATF6 locus that includes portions of the 5′ regulatory region, situated in the 3′ to 5′ orientation within the locus. Another clone, AL391825, contains a more complete L1 sequence overlapping the ATF6 gene. Other BAC clones, such as AC027205 do not contain this L1 sequence. The presence of this L1 insertion in an individual with an autoimmune disease, or one who is suspected to be susceptible to or developing an autoimmune disease, can be detected by isolating DNA from blood or tissue cells, or any other DNA source, from that individual and designing PCR primers that will amplify the L1 insertion in the context of the chromosomal locus of interest. The PCR product is run on an agarose gel and the presence or absence of a band, representing the product of the PCR reaction, observed. The specificity of the PCR amplification can be further increased by performing a nested PCR reaction, in which the PCR product from the first reaction is excised from the gel, passed through a spin column to remove the first pair of primers, and the product then used as a template in a second PCR reaction that uses primers internal to the first set. The presence of a PCR amplified product can then be associated with the presence or absence of an autoimmune disease in a population of patients, or in a subpopulation of patients expressing particular clinical or laboratory features of the disease, and compared to the presence of a similar band in control subjects. Such an L1 element can also be identified using ³²P-labeled DNA probes as in a Southern blot after digestion of DNA with a restriction enzyme. When such an insertion of an L1 element is identified, or when an L1 element is identified proximate to a gene of interest, or a full-length high fidelity L1 element is identified of 98%, 99%, or 100% identity to the L1 consensus sequence regardless of its location, the specific nucleotide sequence of that element can be determined by sequencing the PCR product, subclones of that PCR product, or products that include DNA segments adjacent to the 5′ regulatory region of the L1 element.

Identification of mRNA and Protein Products of L1 Elements

[0126] Transcriptional activity of L1 elements can be assessed by techniques that detect and quantify mRNA encoded by the L1 element ORF1 or ORF2. Production of the protein products of L1 elements can be detected and quantitated by techniques that identify a specific protein. Cells, tissues or body fluids can be isolated from an individual with an autoimmune disease or suspected to be susceptible to or developing an autoimmune disease in order to measure L1 encoded mRNA or protein. Total RNA or poly-A RNA is isolated from the sample, cDNA generated, and specific primers used to amplify the L1 mRNA. As sequences from the 3′ end of L1 elements are often transcribed as “readthrough” transcripts in association with mRNA encoded by other genes, it is most effective to use primer sets that amplify the 5′ regulatory region or 5′ region of the ORF1 product. The presence or absence of a band representing the PCR product can be visualized after running the product on an agarose gel. To more quantitatively assess the mRNA products of L1 elements, a quantitative “mimic” PCR can be performed in which composite mimic primers are designed by incorporating a L1 ORF1 (or ORF2) sequence (from Genbank Accession #U09116) along with a v-erbB fragment provided in a PCR mimic kit (Clontech, Palo Alto, Calif.). For competitive PCR, 1 ml of cDNA, 1 ml of each of 10-fold dilutions of the MIMIC (from 5 to 20 attomoles/ml), 0.5 μl of specific primers, and 22.5 ml of PCR super mix (Life Technologies, Gaithersburg, Md.) are combined and PCR carried out in a thermocycler by denaturing at 94° C. for 45 sec, annealing at 55° C. for 45 sec, and with extension at 72° C. for 1 min. The dilution of mimic which produces a band of equal intensity to that of target DNA is determined. The expression of L1 ORF1 or ORF2 mRNA can also be detected by real-time PCR or by northern blot. In situ hybridization can also be used to detect the mRNAs encoded by L1 elements. In some cases, it may be desirable to induce the expression of L1 mRNA products by treating an individual's cell sample, peripheral blood mononuclear cells for example, with 5-azacytidine or other agents that promote demethylation of DNA prior to isolation of RNA or poly-A RNA. Peripheral blood mononuclear cells can be incubated for 24 to 48 hours with 1-5 mM 5-azacytidine, in the presence or absence of a lymphocyte stimulant such as anti-CD3 and anti-CD28 monoclonal antibodies, RNA or poly-A RNA isolated from the cells, and then competitive mimic PCR or real time PCR performed as described to quantitate L1 ORF1 or ORF2 mRNA. Other agents that promote histone acetylation may also be effective in inducing expression of L1 products.

[0127] Detection of the protein products of L1 elements, either ORF1 or ORF2 gene products, can be used to indicate the presence in cells, tissue, or body fluids of potential immune system triggers that can induce or exacerbate autoimmune disease. Proteins can be detected by several techniques, including immunoprecipitation or Western blot using polyclonal or monoclonal antibodies to the ORF1 or ORF2 products, immunofluorescence or flow cytometry to detect intracellular or cell surface expression of these proteins, immunohistochemistry to detect the proteins in tissue samples, or ELISA to detect L1-encoded ORF1 or ORF1 proteins in plasma, serum, or other body fluids. In some cases, it may be desirable to isolate cells from an individual, as from peripheral blood, and treat those cells with a demethylating agent such as 5-azacytidine (commercially available from Sigma, St. Louis, Mo.) or an agent that promotes histone acetylation (such as suberoylanilide hydroxamic acid or tricostatin A₁, the latter commercially available from Sigma) before isolation of proteins.

Identification of L1 Elements and Products in Ribonucleoprotein Particles

[0128] This section describes the identification of mRNA and protein products of L1 elements, and associated gene sequences, in ribonucleoprotein particles. Characterization of the nucleotide and protein components of ribonucleoprotein particles can be performed to detect the presence of potentially immunostimulatory L1 products, L1 protein products that can serve as autoantigens, or gene sequences that are expressed in association with L1 products and the protein products of which might become immunogenic when expressed in association with those L1 products. Ribonucleoprotein particles can be isolated from cells derived from an individual suspected of having autoimmune disease by preparing cellular extracts and then centrifuging that preparation at 160,000 g for 2.5 h. The protein components of those particles can be characterized by resolving the proteins on a gel, transferring the proteins to a membrane, and then immunoblotting with an antibody specific for predicted protein components. To identify unknown protein components, a band can be excised from the gel and the amino acid sequence determined. The presence of L1 mRNA components in the ribonucleoprotein particles can be detected by generating cDNA followed by PCR amplification using specific primers, or unknown mRNA sequences can be characterized by generating cDNA, followed by direct sequencing. Such RNA transcripts of unknown sequence within ribonucleoprotein particles identify RNA sequences encoded by genes neighboring L1 elements and their protein products represent putative autoantigens.

Detection of Serum Autoantibodies Specific for L1 Products

[0129] Patients with autoimmune disease, particularly those with SLE, often make antibodies with specificity for nucleotide or protein components of intracellular particles, including ribonucleoprotein particles. In accordance with the present invention, the presence of autoantibodies specific for L1 DNA or mRNA sequences or for L1 protein products indicating a diagnosis of autoimmune disease and detection of a change in the titer or level of those antibodies is associated with a change in the clinical disease activity of the patient. Serum autoantibodies specific for L1 products can be detected by the techniques of ELISA, with a recombinant form of the L1 protein product adsorbed to a plastic microwell and then reacted with patient or control serum, or by immunoblot, with patient serum used to detect the L1 DNA, RNA or protein products (FIG. 7) or by immunoprecipitation, in which the patient serum is used to precipitate cellular components containing L1 products, or purified L1 products.

Disease-Specific Examples

[0130] The previous sections have described the general methodology for detecting disease genes, susceptibility to, or diagnosing, complex diseases via L1 element analysis. This section provides strategies for determining susceptibility to specific complex diseases such as autoimmune diseases. Examples include several organ specific autoimmune diseases, in which putative autoantigens can be localized in the genome; SLE, the prototype autoimmune disease; Alzheimer disease, a common dementia in which a region of chromosome 21 has been implicated; and schizophrenia, a common psychotic disease for which recent genome studies have identified genomic loci with statistically significant associations with disease.

Pemphigus Foliaceus

[0131] In order to determine susceptibility to pemphigus foliaceus, the region of chromosome 18q12 encoding desmoglein 1 (sequence in contig NT_(—)010966) and an L1 element with 95% sequence homology to the consensus sequence in the 5′ region is characterized in DNA from study subjects using PCR amplification, a region-specific DNA probe, or by direct DNA sequencing. This L1 element is contained within the coding sequence of DSG1. The results of those assays are compared to results using DNA from control individuals. Expression of desmoglein 1 mRNA or protein in association with L1 mRNA or protein can also be assayed using tissue from skin biopsies. Elevated levels (as described above) of L1 mRNA or protein in serum, plasma, or urine also indicates susceptibility to or diagnosis of autoimmune disease, such as pemphigus.

Autoimmune Thyroid Disease

[0132] In order to determine susceptibility to autoimmune thyroid disease, the region of chromosome 14q31 encoding thyroid stimulating hormone receptor that contains an L1 element with 94% sequence homology to the consensus sequence in the 5′ region contained within the coding region of TSHR on contig NT_(—)010140 is characterized in DNA from study subjects using PCR amplification, a region-specific DNA probe, or by direct DNA sequencing and the results of those assays compared to results using DNA from control individuals. Expression of thyroid stimulating hormone receptor mRNA or protein in association with L1 mRNA or protein can also be assayed using peripheral blood lymphocytes or tissue from thyroid biopsies. Elevated levels (as described above) of L1 mRNA or protein in serum, plasma, or urine also indicates susceptibility to or diagnosis of autoimmune disease, such as autoimmune thyroid disease.

Primary Biliary Cirrhosis

[0133] In order to determine susceptibility to primary biliary cirrhosis, the region of chromosome 13q37 encoding the protein identified as similar to nuclear antigen SP100 protein (LOC93350) (sequence in contig NT_(—)026242) and the nearby L1 element with 95% sequence homology to the consensus sequence in the 5′ region contained within the coding sequence of LOC 93350 is characterized in DNA from study subjects using PCR amplification, a region-specific DNA probe, or by direct DNA sequencing and the results of those assays compared to results using DNA from control individuals. Expression of SP100 mRNA or protein in association with L1 mRNA or protein can also be assayed using peripheral blood lymphocytes or tissue from liver biopsies. Elevated levels (as described above) of L1 mRNA or protein in serum, plasma, or urine also indicates susceptibility to or diagnosis of autoimmune disease, such as primary biliary cirrhosis.

Systemic Autoimmune Diseases

[0134] Systemic autoimmune diseases include, e.g., SLE, mixed connective tissue disease, scleroderma, and Sjögren's syndrome. These autoimmune diseases can be initiated by an immune response to cellular components containing products of L1 elements. The procedure to determine susceptibility to a systemic autoimmune disease is outlined above. Briefly, a map of the location of high fidelity intact L1 elements or full-length L1 elements located in coding regions of genes, or within 100,000 bases of the 5′ or 3′ extent of a gene, is generated, the DNA in those regions of the genome is characterized in subjects being studied for susceptibility to a systemic autoimmune disease, and the number and DNA sequences of those regions compared to healthy control subjects. Alternatively, investigation can be focused on the genomic loci identified in genome screens by microsatellite loci or single nucleotide polymorphism studies, with the full length L1 elements in the approximately 5 million bases on either side of the identified locus searched. The map of full length L1 elements within coding sequences of genes (FIG. 2) and high fidelity full length L1 elements within 100,000 bases of a gene on chromosome 1q serves as an example of the procedure, but all such L1 elements across the genome should be studied (as in Table 3 for all of chromosome 1q and in FIG. 3 for all of chromosome 16). On chromosome 1q, such L1 elements are found in: contig NT_(—)029226 (L1 of 89% identity to consensus sequence in coding sequence of CEZANNE at 150M from ptel); contig NT_(—)4858 (five L1 of 94, 87, 85, 84, and 84% identity to consensus sequence in coding sequence of LOC 128249 at 157.95M from ptel; L1 of 98% identity to consensus sequence within 100,000 bases of FLJ0024 at 167.4 from ptel; L1 of 94 and 91% identity to consensus sequence in coding sequence of NME7 at 170.2-170.5M bases from ptel; L1 of 89% identity to consensus in coding sequence of ATF6 at 162.3M bases from ptel; L1 of 87% identity to consensus in coding sequence of DDR2 at 163.8M bases from ptel; L1 of 88% identity to consensus in coding sequence of ALDH9A1 at 166.75M bases from ptel; and L1 of 81% identity to consensus in coding sequence of KIFAP3 at 171.08M bases from ptel); contig NT_(—)029874 (five L1 of 95, 93, 87, 81, and 79% identity to consensus in LOC127100 at 175.5M bases from ptel); contig NT_(—)029868 (L1 of 98 and 92% identity to consensus sequence in LOC127055 at 178.54M bases from ptel); contig NT_(—)026949 (L1 of 83% identity to consensus in FLJ10244 at 180.6M bases from ptel; L1 of 89% identity to consensus in NPHS2 at 181.8M bases from ptel); contig NT_(—)004552 (L1 of 99% identity to consensus in XPR1 at 184.28M bases from ptel); contig NT_(—)029219 (L1 of 98% identity to consensus within 100,000 bases of LOC126918 at 186.7M bases from ptel); contig NT_(—)004487 (L1 of 98% identity to consensus in coding sequence of C1ORF24, NIBAN, at 189M bases from ptel; L1 of 98% identity to consensus within 100,000 bases of LOC 127523 at 191.9M bases from ptel; L1 of 98% identity to consensus within 100,000 bases of LOC127522 and LOC127521 at 191.5M bases from ptel; L1 of 91% identity to consensus within coding sequence of FIBL-6 at 190.65M bases from ptel); contig Nt_(—)004671 (l1 OF 98% identity to consensus within coding sequence of LOC127964 at 198.58M bases from ptel); contig NT_(—)004416 (L1 of 99% identity to consensus within 100,000 bases of LOC127387 at 202.65M bases from ptel; L1 of 93% identity to consensus within coding sequence of LOC127388 at 202.5M bases from ptel); contig NT_(—)029862 (L1 with 98% identity to consensus witin 100,000 bases of LOC127012 at 204.1M bases from ptel; L1 of 88% identity to consensus in coding sequence of FHR5 at 203.22M bases from ptel; L1 of 96% identity to consensus in coding region of F13B at 203.26M bases from ptel); contig NT_(—)021877 (L1 of 98% identity to consensus in coding sequence of LOC126615 at 217M bases from ptel); contig NT_(—)030578 (four L1 with 90, 89, 88, and 83% identity to consensus in coding sequence of KCNH1 at 217.84-218.23M bases from ptel); contig NT_(—)004993 (L1 with 85% identity to consensus in coding sequence of FLJ10874 at 119.68M bases from ptel); contig NT_(—)004817 (L1 of 98% identity to consensus within 100,000 bases of LOC128150 and LOC 128149 at 224.6M bases from ptel; L1 of 88% identity to consensus within coding sequence of FLJ10252 at 225.3M bases from ptel); contig NT_(—)029871 (L1 with 96% identity to consensus in coding sequence of RAB3-GAP150 at 228.1M bases from ptel); contig NT_(—)004861 (L1 with 85% identity to consensus in coding sequence of FLJ10052 at 231.6M bases from ptel); contig NT_(—)004753 (L1 with 91% identity to consensus in coding sequence of DISC1 at 238.61 to 239.13M bases from ptel); contig NT_(—)004836 (L1 of 99% identity to consensus in coding sequence of RYR2 at 244.3M bases from ptel; L1 of 88% identity to consensus in coding sequence of TM7SF1 at 243.38M bases from ptel); contig NT_(—)004771 (L1 of 98% identity to consensus within 100,000 bases of LOC114922 at 248.38M bases from ptel; L1 of 91% identity to consensus in coding sequence of RGS7 at 247.52M bases from ptel); contig NT_(—)004734 (L1 of 86% identity to consensus in coding sequence of AKT3 at 251.3 to 251.4M bases from ptel); and contig NT_(—)004536 (L1 of 99% identity to consensus within 100,000 bases of LOC127615 and LOC127616 at 252.6M bases from ptel; L1 of 95% identity to consensus within coding sequence of FLJ21080 at 254.2M bases from ptel). These identified genes and predicted genes represent candidate disease genes from among the approximately 1600 genes and predicted genes on human chromosome 1q and as such, may warrant consideration for further study for involvement in the pathogenesis of autoimmune and other diseases, for involvement in a molecular pathway involved in the pathogenesis of autoimmune and other diseases, for susceptibility genes for these diseases, and as potential targets for therapy of such diseases. Similar analyses can be performed in any other region of the genome. Each of these chromosomal regions can be characterized in a study subject by PCR amplification, a region-specific DNA probe, or by direct DNA sequencing and the results of those assays compared to results using DNA from control individuals. The presence of an increased number of productive L1 sequences in an individual's genome or in the coding regions, particularly intronic regions, of genes would be associated with increased susceptibility to systemic autoimmune disease or other disease.

[0135] In addition to increased numbers of productive L1 elements, altered expression of a gene product implicated in immune system function, inflammation, or other pathway relevant to pathogenesis of autoimmune disease based on proximity of an L1 element to that gene may confer susceptibility to systemic autoimmune diseases. A map of genes may that are proximate to L1 elements can be constructed and the DNA sequences in those regions be determined by characterizing DNA from study subjects using PCR amplification, a region-specific DNA probe, or by direct DNA sequencing and the results of those assays compared to results using DNA from control individuals. For example, to determine susceptibility to SLE, the region of chromosome 1q encoding FcgRIIb (contig NT_(—)004668) and the nearby L1 element with 89% sequence homology to the consensus sequence in the 5′ region and contained within the coding sequence of ATF6, a cAMP dependent transcription factor, is characterized in DNA from study subjects using PCR amplification, a region-specific DNA probe, or by direct DNA sequencing and the results of those assays compared to results using DNA from control individuals. The presence of the L1 element in this region would predict susceptibility to SLE.

Alzheimer Disease

[0136] Identification of genes and genes products relevant to the pathogenesis of Alzheimer disease, and identification of individuals susceptible to developing the disease, can be determined by using a similar approach to that described for chromosome 1q, with the study directed toward those genomic regions that have been implicated in the disease. The entire genomic sequence of chromosome 21, which had been associated with a diagnosis of Alzheimer disease in family studies and sporadic cases, was analyzed for expression of full length and full length high fidelity L1 sequences within 100,000 bases of a gene or predicted gene. FIG. 4 shows the results of such analysis and demonstrates only three positive results: on contig NT_(—)011512 (L1 of 97% identity to consensus in coding sequence of APP at 23.0M bases from ptel; L1 of 90% identity to consensus in coding sequence of TTC3 at 35.1M bases from ptel; two L1 of 93 and 87% identity to consensus in coding sequence of DSCAM at 38.1M bases from ptel). APP encodes amyloid precursor protein, documented to be mutated in some familial cases of Alzheimer disease and proposed to be involved in a common pathogenic pathway in Alzheimer disease. The other two identified genes, are also excellent candidates for disease genes. TTC3 encodes a protein with a tetratricopeptide domain and DSCAM is Down's syndrome cell adhesion molecule.

Schizophrenia

[0137] Identification of genes that may be important in the pathogenesis of schizophrenia are similarly identified by analysis of chromosomal regions adjacent to loci statistically associated with a diagnosis of schizophrenia. Table 2 lists some susceptibility loci and some candidate genes, based on analysis of L1 sequences. These loci and associate candidate genes include: DIS196 located 170.1M bases from ptel on chromosome 1 with associated L1-containing genes KIFAP1 (kinensin associated protein, expressed in cerebellum, at 171.08M bases from ptel) and DDR2 (neurotrophic receptor tyrosine kinase receptor related protein at 163.8M bases from ptel); D4S430 located 115.4M bases from ptel on chromosome 4 with associated gene CAMK2D (calcium calmodulin delta 2 kinase, expressed in hippocampal and pyramidal cells at 113.9M bases from ptel); D5S422 located 167.97 from ptel on chromosome 5 and associated gene GLRA1 (glycine receptor alpha 1, implicated in startle disease and stiff man syndrome, at 161.9M bases from ptel); D8S503 located 7.28M bases from ptel on chromosome 8 and associated genes DLGAP2 (concentrated in synaptic junctions and in hippocampus at 1.5M bases from ptel), CSMD1 (with domains abundant in complement control proteins at 3.8M bases from ptel), and FDFT1 (famesyltransferase, active in cholesteral biosynthesis, at 12.1M bases from ptel); D8S1771 located 26.48M bases from ptel on chromosome 8 and associated genes BNIP3L (a proapoptotic protein at 27.3M bases from ptel), LOC137822 (gene with protein of unknown function containing an L1 with 99% identity to the consensus in its coding region, at 27.9M bases from ptel), and WRN.RECQL2 (Werner's syndrome gene at 31M bases from ptel); D11S934 located 132.8M bases from ptel and associated genes TEKTA (encoding a protein associated with deafness at 129M bases from ptel) and GRIK4 (a glutamate receptor gene expressed in brain located at 129M bases from ptel); and D20S112 located at 17.25M bases from ptel with associated genes PGAM-B (similar to brain phosphoglycerate mutase at 9.55M bases from ptel) and LOC96688 (a neuroendocrine convertase 2 precursor at 17.16M bases from ptel). All of these genes containing full length L1 elements in their coding regions and are proposed as potential candidate disease genes in schizophrenia.

Prevention and Treatment of Complex Diseases

[0138] According to the invention, inhibition of the expression or function of L1 mRNA or protein products can be used to prevent or treat autoimmune diseases. The expression of relatively increased cellular levels of mRNA transcripts of L1 elements, the protein products of ORF1 or ORF2, or L1 mRNA products in close association with mRNA or protein products of other host genes can confer an autoimmune or other pathogenic state on an individual. Therefore, decreasing the quantity or activity of such L1 products in order to inhibit or decrease the disease activity in an individual patient, or to prevent the initiation of autoimmune disease in a susceptible individual is a preferred embodiment of the present invention.

[0139] There are many standard or proposed approaches to inhibiting the expression or function of gene products, including both mRNA and protein products. These approaches can act on the conformation or biochemical composition of DNA or the proteins, such as histones, that associate with DNA. Promotion of DNA methylation, inhibition of DNA demethylation, promotion of histone deacetylation, and inhibition of histone acetylation are examples of such approaches. Transcription factors that bind to regulatory DNA elements can be specifically targeted to inhibit gene transcription. mRNA can either be specifically inhibited using agents such as anti-sense, or mRNA stability can be manipulated by augmenting or inhibiting proteins that bind to the specific mRNA and modify the degradation of that mRNA.

[0140] For example, hypermethylation mediated by proteins such as DNA-methyltransferase is associated with transcriptional inactivation in both normal cells and in some cancers (73, 74, 75). Demethylation with 5-aza can restore gene transcription (75). Alternatively, histone acetyltransferases contribute to relaxation of chromatin structure and gene transcription (76), and histone deacetylases can function as transcriptional repressors (77). Biochemical modifiers of this process include suberoylanilide hydroxamic acid, a histone deacetylase inhibitor, or trichostatin (78). Transcription factors that bind to regulatory DNA elements can be specifically targeted to inhibit gene transcription. The SRY protein is an example of a protein that inhibits transcription of L1 elements. mRNA can be specifically inhibited or degraded using agents such as anti-sense or mediators of RNA interference (79). mRNA stability can also be manipulated by augmenting or inihibiting proteins that bind to the specific mRNA and modify the degradation of that mRNA. For example, proteins that bind to the 3′ untranslated region of an mRNA and stabilize that mRNA, the suggested role of members of the HuR family of proteins, might be inhibited, or proteins that mediate mRNA degradation, such as tristetraprolin, might be induced (80, 81). It should be noted that the state of the art regarding regulation of mRNA stability does not at present define all proteins that regulate mRNA stability or their functions.

[0141] The protein products of L1 elements, the ORF1 and ORF2 proteins, can also be targeted for inhibition by antibodies, such as specific monoclonal antibodies, or small protein inhibitors that block the actions of those proteins. Therapeutic inhibition of the mRNA or protein products of L1 elements is expected to decrease the availability or activity of the immunologic stimulus for autoimmune disease, to improve the clinical activity of that disease, or to inhibit the initiation of the initial disease state. In one embodiment, monoclonal antibodies immunoreactive with the ORF1 and/or ORF2 proteins are generated using routine procedures well known to those of ordinary skill in the art.

Monoclonal Antibodies Against ORF1 and/or ORF2

[0142] The general methodology for making monoclonal antibodies by hybridomas is well known. See, e.g., Kohler et al., 1980, Hybridoma Techniques, Cold Spring Harbor Laboratory, New York; Tijssen, 1985, Practice and Theory of Enzyme Immunoassays, Elsevier, Amsterdam; Campbell, 1984, Monoclonal Antibody Technology, Elsevier, Amsterdam; Hurrell, 1982, Monoclonal Hybridoma Antibodies: Techniques and Applications, CRC Press, Boca Raton, Fla. Purification methods for antibodies are disclosed, e.g., in The Art of Antibody Purification, 1989, Amicon Division, W.R. Grace & Co.

[0143] In a preferred embodiment, when the antibodies are used therapeutically to treat humans, the antibodies are “humanized”, i.e., human Fc sequences are present in the antibody molecule to prevent an adverse immune response in a patient to whom the antibodies are administered. When used to treat patients suffering from a complex disease as defined herein, such antibodies can be administered in amounts effective to treat or prevent the manifestation of the symptoms of these diseases. These effective amount broadly ranges between about 1 and 1000 mg per kg body weight of said mammal. The antibodies can be administered systemically, preferably parenterally and most preferably subcutaneously or intravenously.

Other Ligands

[0144] The identification of L1 elements provides for development of screening assays, particularly for high throughput screening of molecules that modify, up- or down-regulate, i.e., inhibit or stimulate, agonize or antagonize, the transcription or translation activity of the L1 element. Alternatively, anti-sense oligonucleotides can be used to prevent L1 transcripts from translation, or to prevent L1 transcipts, ORF1, or ORF2 from associating to susceptibility genes, their corresponding mRNA or translation products. The present invention contemplates screens for small molecule ligands or ligand analogs and mimics, as well as screens for natural ligands to L1 molecules.

[0145] Any screening technique known in the art can be used to screen for compounds which up- or down-regulates the transcription or translation activity of the L1 element. For instance, a screening assay can be based on measurement of the amount or formation rate of transcribed L1 mRNA by a suitable method, or transcription of the L1 gene resulting in the formation or release of a reporter molecule which can be easily measured. Generally, a screening assay involves contacting the L1 gene, mRNA, or protein sequence with a compound which interacts or otherwise affects the conformation or activity of the sequence. The L1 promoter sequence can be linked to cDNA encoding for a reporter protein, or another polypeptide or protein. The transcriptional activity of the promoter is measured in the presence of the compound, and compared to a control value. This control value could be, for example, transcriptional activity of the promoter in the absence of the compound, transcriptional activity of the promoter in the presence of a reference compound with a known effect on transcriptional activity, or another theoretically or experimentally derived value.

Gene Therapy to Modulate L1 Transcription or Biological Activity

[0146] A LINE gene such as L1, or alternatively a negative regulator of the L1 element such as an antisense nucleic acid, intracellular antibody (intrabody), can be introduced in vivo, ex vivo, or in vitro using a viral or a non-viral vector, e.g., as discussed above. Expression in targeted tissues can be effected by targeting the transgenic vector to specific cells, such as with a viral vector or a receptor ligand, or by using a tissue-specific promoter, or both. Targeted gene delivery is described in International Patent Publication WO 95/28494, published October 1995.

[0147] Preferably, for in vivo administration, an appropriate immunosuppressive treatment is employed in conjunction with the viral vector, e.g., adenovirus vector, to avoid immuno-deactivation of the viral vector and transfected cells. For example, immunosuppressive cytokines, such as interleukin-12 (IL-12), interferon-γ (IFN-γ), or anti-CD4 antibody, can be administered to block humoral or cellular immune responses to the viral vectors (see, e.g., Wilson, Nature Medicine, 1995). In that regard, it is advantageous to employ a viral vector that is engineered to express a minimal number of antigens.

[0148] Adenovirus Vectors.

[0149] Adenoviruses are eukaryotic DNA viruses that can be modified to efficiently deliver a nucleic acid of the invention to a variety of cell types in vivo, and has been used extensively in gene therapy protocols. Various serotypes of adenovirus exist. Of these serotypes, preference is given to using type 2 or type 5 human adenoviruses (Ad 2 or Ad 5) or adenoviruses of animal origin (see W094/26914). Those adenoviruses of animal origin which can be used within the scope of the present invention include adenoviruses of canine, bovine, murine (example: Mavl, Beard et al., Virology 75 (1990) 81), ovine, porcine, avian, and simian (example: SAV) origin. Preferably, the adenovirus of animal origin is a canine adenovirus, more preferably a CAV2 adenovirus (e.g., Manhattan or A26/61 strain (ATCC VR-800), for example). Various replication defective adenovirus and minimum adenovirus vectors have been described for gene therapy (WO94/26914, WO95/02697, WO94/28938, WO94/28152, WO94/12649, WO95/02697 WO96/22378). The replication defective recombinant adenoviruses according to the invention can be prepared by any technique known to the person skilled in the art (Levrero et al., Gene 101:195 1991; EP 185 573; Graham, EMBO J. 3:2917, 1984; Graham et al., J. Gen. Virol. 36:59 1977). Recombinant adenoviruses are recovered and purified using standard molecular biological techniques, which are well known to one of ordinary skill in the art.

[0150] Adeno-Associated Viruses.

[0151] The adeno-associated viruses (AAV) are DNA viruses of relatively small size which can integrate, in a stable and site-specific manner, into the genome of the cells which they infect. They are able to infect a wide spectrum of cells without inducing any effects on cellular growth, morphology or differentiation, and they do not appear to be involved in human pathologies. The AAV genome has been cloned, sequenced and characterized. The use of vectors derived from the AAVs for transferring genes in vitro and in vivo has been described (see WO 91/18088; WO 93/09239; U.S. Pat. Nos. 4,797,368, 5,139,941, EP 488 528). The replication defective recombinant AAVs according to the invention can be prepared by co-transfecting a plasmid containing the nucleic acid sequence of interest flanked by two AAV inverted terminal repeat (ITR) regions, and a plasmid carrying the AAV encapsidation genes (rep and cap genes), into a cell line which is infected with a human helper virus (for example an adenovirus). The AAV recombinants which are produced are then purified by standard techniques.

[0152] Retrovirus Vectors.

[0153] In another embodiment the gene can be introduced in a retroviral vector, e.g., as described in Anderson et al., U.S. Pat. No. 5,399,346; Mann et al., 1983, Cell 33:153; Temin et al., U.S. Pat. No. 4,650,764; Temin et al., U.S. Pat. No. 4,980,289; Markowitz et al., 1988, J. Virol. 62:1120; Temin et al., U.S. Pat. No. 5,124,263; EP 453242, EP178220; Bernstein et al. Genet. Eng. 7 (1985) 235; McCormick, BioTechnology 3 (1985) 689; International Patent Publication No. WO 95/07358, published Mar. 16, 1995, by Dougherty et al.; and Kuo et al., 1993, Blood 82:845. The retroviruses are integrating viruses which infect dividing cells. The retrovirus genome includes two LTRs, an encapsidation sequence and three coding regions (gag, pol and env). In recombinant retroviral vectors, the gag, pol and env genes are generally deleted, in whole or in part, and replaced with a heterologous nucleic acid sequence of interest. These vectors can be constructed from different types of retrovirus, such as MoMuLV (“murine Moloney leukaemia virus”), MSV (“murine Moloney sarcoma virus”), HaSV (“Harvey sarcoma virus”); SNV (“spleen necrosis virus”); RSV (“Rous sarcoma virus”) and Friend virus. Suitable packaging cell lines have been described in the prior art, in particular the cell line PA317 (U.S. Pat. No. 4,861,719); the PsiCRIP cell line (WO 90/02806) and the GP+envAm-12 cell line (WO 89/07150). In addition, the recombinant retroviral vectors can contain modifications within the LTRs for suppressing transcriptional activity as well as extensive encapsidation sequences which may include a part of the gag gene (Bender et al., J. Virol. 61:1639, 1987). Recombinant retroviral vectors are purified by standard techniques known to those having ordinary skill in the art.

[0154] Retrovirus vectors can also be introduced by recombinant DNA viruses, which permits one cycle of retroviral replication and amplifies transfection efficiency (see WO 95/22617, WO 95/26411, WO 96/39036, WO 97/19182).

[0155] Lentivirus Vectors.

[0156] In another embodiment, lentiviral vectors are can be used as agents for the direct delivery and sustained expression of a transgene in several tissue types, including brain, retina, muscle, liver and blood. The vectors can efficiently transduce dividing and nondividing cells in these tissues, and maintain long-term expression of the gene of interest. For a review, see, Naldini, Curr. Opin. Biotechnol., 9:457-63, 1998; see also Zufferey, et al, J. Virol., 72:9873-80, 1998). Lentiviral packaging cell lines are available and known generally in the art. They facilitate the production of high-titer lentivirus vectors for gene therapy. An example is a tetracycline-inducible VSV-G pseudotyped lentivirus packaging cell line which can generate virus particles at titers greater than 106 IU/ml for at least 3 to 4 days (Kafri, et al., J. Virol., 73: 576-584, 1999). The vector produced by the inducible cell line can be concentrated as needed for efficiently transducing nondividing cells in vitro and in vivo.

[0157] Non-Viral Vectors.

[0158] A vector can be introduced in vivo in a non-viral vector, e.g., by lipofection, with other transfection facilitating agents (peptides, polymers, etc.), or as naked DNA. Synthetic cationic lipids can be used to prepare liposomes for in vivo transfection, with targeting in some instances (Feigner, et. al., Proc. Natl. Acad. Sci. U.S.A. 84:7413-7417, 1987; Feigner and Ringold, Science 337:387-388, 1989; see Mackey, et al., Proc. Nati. Acad. Sci. U.S.A. 85:8027-8031, 1988; Ulmer et al., Science 259:1745-1748, 1993). Useful lipid compounds and compositions for transfer of nucleic acids are described in International Patent Publications WO95/18863 and WO96/17823, and in U.S. Pat. No. 5,459,127. Other molecules are also useful for facilitating transfection of a nucleic acid in vivo, such as a cationic oligopeptide (e.g., International Patent Publication WO95/21931), peptides derived from DNA binding proteins (e.g., International Patent Publication WO96/25508), or a cationic polymer (e.g. , International Patent Publication WO95/21931). Recently, a relatively low voltage, high efficiency in vivo DNA transfer technique, termed electrotransfer, has been described (Mir et al., C.P. Acad. Sci., 321:893, 1998; WO 99/01157; WO 99/01158; WO 99/01175). DNA vectors for gene therapy can be introduced into the desired host cells by methods known in the art, e.g., electroporation, microinjection, cell fusion, DEAE dextran, calcium phosphate precipitation, use of a gene gun (ballistic transfection), or use of a DNA vector transporter (see, e.g., Wu et al., J. Biol. Chem. 267:963-967, 1992; Wu and Wu, J. Biol. Chem. 263:14621-14624, 1988; Hartmut et al., Canadian Patent Application No. 2,012,311, filed Mar. 15, 1990; Williams et al., Proc. Natl. Acad. Sci. USA 88:2726-2730, 1991). Receptor-mediated DNA delivery approaches can also be used (Curiel et al., Hum. Gene Ther. 3:147-154, 1992; Wu and Wu, J. Biol. Chem. 262:4429-4432, 1987). U.S. Pat. Nos. 5,580,859 and 5,589,466 disclose delivery of exogenous DNA sequences, free of transfection facilitating agents, in a mammal.

[0159] The knowledge derived from the procedures described above would allow for better diagnostic procedures for identifying individuals at risk for, susceptible to, or predisposed to complex diseases in which an L1 element is a direct or indirect factor. The correlation between the distance of an L1 element from, or the presence of an L1 element in an intron sequence of, a susceptibility gene, to disease susceptibility and progression, will provide for a better understanding of the causes and progression of autoimmune and other complex diseases, as well as novel therapeutic strategies for treating such diseases.

EXAMPLES

[0160] The present invention will be better understood by reference to the following Examples, which are provided as exemplary of the invention, and not by way of limitation.

Example 1 Identification of High Fidelity L1 Elements along Chromosome 1q

[0161] Chromosome 1q BAC clones, or contig clones (combining sequences from several BAC clones placed in proper order), were identified and ordered based on the contigs or BACs listed in the NCBI database, along with BACs or contigs identified by BLAST searching chromosome 1q microsatellite markers against the non-redundant and hgts human sequence databases.

[0162] Using the BLAST program for comparison of two sequences, all 80contigs on chromosome 1q, containing about 1600 genes, were compared to the 5′ and ORF1 sequence of LRE2, the L1 element previously localized to chromosome 1q and derived from a mutagenic insertion into a dystrophin gene (accession U09116). Of those clones, some were found to contain at least partial 5′ L1 sequences, while most included 3′ fragments. 26 genes were found to contain full length L1 sequences in their coding regions and some additional L1 sequences were found in close proximity (within 100,000bases) of a gene or predicted gene (see Table 3). These 26 genes were chosen for further study.

Example 2 Relationship of High Fidelity L1 Elements to SLE Susceptibility Loci

[0163] To relate the identified full-length high fidelity L1 sequences to previously identified SLE susceptibility loci, the data from several genome screens using microsatellite markers were relied upon (3, 4, 91). With increased availability of chromosome 1q BAC sequences, the precise location of the various microsatellite markers can be tied to specific BAC clones and localized along the chromosome more accurately than previously, although the location of some markers defined by radiation hybrid analysis remains ambiguous. Markers demonstrated by several investigators to characterize susceptibility loci were located using the chromosome mapping database available through NCBI.

[0164] Of 10 chromosome markers localized, 6 were within 1.7 cM of a potentially active L1 element. The 3 other loci, including the FCGR2A and MHC loci, may be associated with SLE through a mechanism that does not involve L1 elements. Alternatively, the disease marker may reflect the proximity of a gene in which a full-length, but not 98% or 99% identical to consensus, L1 element is included within the intronic region of a nearby gene. This is the case for FCGR2A, with an 89% identical to consensus L1 element in the intron of ATF6, immediately adjacent to FCGR2A, and with an 87% identical to consensus element in an intronic region of DDR2, approximately 1.2M bases from FCGR2A. The same may be true of D6S2410, which has LOC94915, a gene with possible calmodulin like calcium binding domains, at 1.63M bases from the marker and having an intronic L1 with 86% identity to the consensus sequence.

[0165] A more thorough analysis of chromosome 16 has identified additional candidate disease genes as indicated in FIG. 3 and Table 4. Notable candidate genes include ITGAM at 32M bases from ptel and with an 88% identical to consensus L1 in an intron; PHKB at 47.7M bases from ptel and with 3 L1 elements with 90%, 85%, and 82% sequence identity to the consensus; cadherin 8, at 64.3M bases from ptel with 3 L1 elements with 97%, 96%, and 95% sequence identity to consensus; and CDH13, a cadherin expressed in heart, at 87.1M bases from ptel with a 99% identical to consensus L1 element in an intronic region.

Example 3 Variability in 5′ Regulatory Region of L1 Elements

[0166] The data in the previous Examples showed an association between the location of high fidelity L1 elements and SLE susceptibility loci. To address the basis of this variability that may be linked to microsatellite markers in individuals, the literature was first considered and a comparison initiated of replicate copies of a single L1 element available in the database. Kazazian's group had documented polymorphism in expression of particular active L1 elements, as well as sequence variability in those that are expressed (43). For example, the gene frequency of LRE2 in the diploid genome was estimated at 0.65 and that of L1.3, on chromosome 14, of only 0.15. Thus, disease susceptibility is increased by the presence of an active element in an individual susceptible to SLE and relative protection is conferred by the absence of the active L1 in an individual.

[0167] An initial effort to study sequences more 5′ to the published consensus 5′ sequence used a several hundred bp sequence 5′ of a high fidelity L1 element located on chromosome 1q (AL162431, with 99% homology to the 5′ of U09116). This L1 may represent the genomic equivalent of LRE2 and is particularly intriguing as it is adjacent to the gene for a cell surface retroviral receptor. This 5′ region identified numerous BAC clones with high fidelity L1 elements.

Example 4 Identification of L1 Elements Adjacent to Disease-Relevant Genes

[0168] While the previous Examples localized predicted L1 sequences with the capacity to produce full length coding region RNAs, as well as ORF1, and possibly ORF2, proteins, it is the particles containing those components that would be immunogenic. However, one of the chromosome 1q loci that has received the most attention and some strong support for linkage to SLE, containing the Fcg receptor genes, did not show a nearby high fidelity L1 element (the calculated distance for FCGR2A was 4.78M bp). In view of the variable expression of some L1 elements among individuals, it is possible that the BAC clones from the FcR region published in the database does not include such a sequence, yet a clone from another individual over the same interval might. Eight overlapping clones were present at 162.3M bases from ptel of 1q. When compared to the 5′ and ORF1 L1 consensus sequence, no significant similarity was detected by the BLAST program for 5 of the BAC clones while one clone (AL391825) contained a full length L1 element with 89% identity to the consensus. Two other clones had a partial L1 sequence (AC027205 and AL359541). This locus may reflect the polymorphic expression of an L1 element in some individuals but not others.

[0169] The presence of a low fidelity L1 sequence within or near a disease-relevant gene raised the possibility that the third or fifth proposed mechanism for induction of human disease by L1 elements might pertain to SLE. Gene expression, function, or immunogenicity might be modulated by virtue of the presence of the regulatory L1 sequences or by coordinated transcription of both L1 and adjacent genes. To begin to investigate a potential disease-related role for L1 elements in genes that have been studied in conjunction with organ-targeted immune responses, the thyroid stimulating hormone receptor gene on chromosome 14q31, 79.6M bases from ptel, was analyzed. Contig NT_(—)010140 contained a full length L1 element with 94% identity to the consensus L1 sequence within an intronic region. Similarly, the DSG1 gene, an autoantigen for pemphigus foliaceous, on chromosome 18q12, contains a full length L1 element of 95% sequence identity to the consensus in an intronic region. The expression of L1 sequences within intronic segments of a gene may confer increased immunogenicity on that gene product.

Example 5 Expression of Orf1 mRNA and Protein

[0170] Enrichment of a genome in transcriptionally competent L1 elements would be predicted to result in detectable expression of L1 mRNA, and might also contribute to production of p40 and reverse transcriptase proteins. Cellular expression of ORF1p40 is seen in several teratocarcinoma cell lines, including NTERA-D1 (54). Several hints in the literature also suggest that some lymphocyte cell lines might express L1 p40 (55). Consistent with possible production of this protein in lymphocytes, it has been suggested that L1 products might serve an important cellular function in the repair of double stranded breaks, as occur in the setting of VDJ recombination or immunoglobulin class switching (60). For study of L1 product expression in SLE, three assays have been established, competitive mimic PCR to detect ORF1 p40 mRNA, real time PCR to detect ORF1 p40 mRNA, and Western blot to detect p40 protein. Total cellular RNA was isolated from NTERA teratocarcinoma cells or from HeLa cells, treated with DNAse followed by column purification to remove genomic DNA, reverse transcribed into cDNA, and then amplifed by PCR in the presence of 0.2-20 attomoles of a mimic construct containing a segment of the ORF1 coding sequence. In this assay, the relative concentration of target (cellular ORF1 cDNA) can be determined by noting the mimic concentration which is outcompeted by target (FIG. 5). While HeLa cells showed only trace concentrations of ORF1 cDNA, NTERA ORF 1 was readily detected. CpG motifs are present in the 5′ regulatory region of L1, and it has been proposed that demethylation might contribute to transcriptional activation. To determine if demethylation modulates ORF1 mRNA expression by HeLa or NTERA cells, the cells were treated with 0.5, 1.0, or 4 mM 5-azacytidine (5-Aza), known to result in demethylation of CpG dinucleotides. 4 mM 5-Aza induced a modest increase in ORF 1 mRNA in HeLa cells, while the already evident mRNA in NTERA cells was increased by even 0.5 mM 5-Aza. It is interesting to note that some lupus-inducing drugs mediate DNA demethylation, and 5-Aza can induce self-reactive T cells and lupus-like disease in an animal model (66). Induction of L1 gene activation might be a mechanism that accounts for these effects of DNA demethylation. Studies have also been initiated to study expression of ORF1 mRNA in lymphoid cell lines. Product has been detected in the D1.1 Jurkat cell variant and the CL-01 Burkitt's lymphoma B cell line in preliminary experiments.

[0171] As noted, readthrough transcripts of cellular genes that also contain fragments of L1 sequence are ubiquitous. While most of those background sequences are derived from ORF2, while we are amplifying ORF 1, all future experiments will be performed using polyA RNA rather than total cellular RNA, to enrich for those mRNAs specific to ORF 1.

[0172] To detect ORF1 protein, a Western blot was established which uses a rabbit antibody specific for ORF1 and is preadsorbed to remove nonspecific reactivities (54). Immunoblot analysis of protein extracts from HeLa and NTERA cells showed several nonspecific high molecular weight bands, also reported in the literature, along with a strong 40 kD band in NTERA (FIG. 6.A). A weak 40 kD band was also observed in HeLa cells in some experiments. As functional ORF1 p40 protein has been shown to be enriched in cytoplasmic RNP particles, that fraction was isolated by ultracentrifugation. In some experiments, the purification step resulted in a marked enrichment in the p40 protein band, while in others that fraction showed some additional degradation products. The RNP particle fraction can be used to increase the sensitivity of detection of the p40 protein in future experiments.

Example 6 Analysis of ORF1 Protein in SLE Lymphocytes

[0173] Increased expression of ORF 1 mRNA and protein would reflect either increased number and/or transcriptional activity of the complement of intact L1 elements in an individual's genome. It is therefore possible to detect those products. An important issue, however, is the choice of cell population for detection of ORF1 products. Ovary is predicted to be most enriched in ORF1 protein in females, but this tissue is rarely accessible. Based on the preliminary data showing ORF1 mRNA in several lymphoid cells lines, as well as some similar data in the literature, it was considered that lymphoid cells might on occasion express L1 protein (55). The speculation by others that L1 products might assist in the DNA repair process during events such as immunoglobulin class switching was intriguing in view of the augmented class switching in SLE that contributes to generation of pathogenic IgG autoantibodies.

[0174] Peripheral blood T and non-T cell fractions were isolated from 4 SLE patients and several healthy individuals, protein extracts subjected to ultracentrifugation to enrich for RNP particles, and that fraction analyzed by western blot. In these preliminary experiments, while no p40 bands have been observed in samples from controls, one of the four SLE non-T cell preparations showed a clear 40 kD protein detected with the anti-ORF1 antiserum (FIG. 6B). T cells from that individual were negative.

[0175] In another experiment (FIG. 6.C), non-T cells from all three SLE patients studied showed a band of approximately 40 kD after immunoblotting with the anti-p40 antibody, while non-T cells from a healthy control, and the T cell fractions from all subjects, were negative for a 40 kD band

Example 7 Production of L1 ORF1 mRNA and Protein in SLE Lymphocytes

[0176] In view of the intriguing finding of p40 protein in the non-T cell fraction of 1 of 4 SLE patients studied, p40 mRNA and protein in T and non-T cell fractions from normal peripheral blood and human tonsil cells are compared. Normal peripheral blood T and B cells can be negative, while tonsil B cells can give some signal. The tonsil cells are fractionated into those with GC phenotype based on expression of typical cell surface markers to define the cell subset producing L1 products. ORF1 mRNA and p40 protein expression in SLE peripheral blood T and non-T cells is explored, as well as in RA and healthy controls. The presented model requires production of both L1 RNA and protein at some cellular site in SLE. Studies are therefore designed to investigate whether these L1 products are produced throughout the course of disease, or only during the initiation phase.

[0177] Individuals recently diagnosed with SLE and followed in the SLE Pediatric Rheumatology Clinic at HSS where active SLE patients between the ages of 14 and 20 are seen regularly are initially studied. A Pediatric SLE DNA Repository containing family member DNA is available for correlative DNA analyses. Initially, 20 patients with recent onset SLE, 20 RA patients, and 20 healthy controls are studied for expression of ORF1 mRNA and protein as described. Some samples undergo enrichment for the RNP fraction by ultracentrifugation prior to Western blot analysis. In additional experiments, cell fractions will be preincubated for 48 hours with 5-Aza, in the presence or absence of physiologically relevant stimuli (anti-CD3, commercially available from ATCC, Menassus, Va. +anti-CD28 mAbs or F(ab′)2 anti-IgM+recombinant human CD40 ligand), prior to RNA or protein extraction.

Example 8 Detection of Autoantibodies to L1 ORF1 p40 protein

[0178] Patients with systemic autoimmune disease produce antibodies to nucleic acids and their associated proteins contained within intracellular particles. Support for a role for L1 elements and their products in the induction of autoimmune disease would be provided by documentation of autoantibodies specific for L1 encoded proteins. A recombinant fusion protein comprising p40 protein tagged with 6 histidines was produced and used to study SLE and healthy control sera for the presence of anti-p40 autoantibodies by electrophoresing the recombinant p40 protein on a gel and performing a western blot procedure with sera. A band representing reactivity of immunoglobulin with the p40 protein was detected in sera from SLE patients and a serum sample from an MRL/lpr lupus mouse, but not in several normal sera and only very weakly in another normal serum sample (FIG. 7).

Search for Genes and Gene Products Involved in Alzheimer Disease

[0179] Studies of extended families with early-onset Alzheimer disease provided support for an association of individual variability on chromosome 21 with that disease. To define the location of full-length high fidelity L1 elements in proximity to genes and the location of L1 elements included in intronic or untranslated regions of genes, the entire published sequence of chromosome 21 was searched. First, the public genome database available through NCBI was accessed and a list of all of the available contigs that covered that chromosome generated. In the case of chromosome 21, the majority of the sequence is included in a single contig with accession number NT_(—)011512. That large sequence was directly searched, and in addition, a list of the BAC clones that comprise the contig was generated in order to sequentially search the genome seqments that make up the larger contig. The search could also have been focused on the region of the chromosome neighboring published microsatellite markers associated with the disease.

[0180] A publicly available search program, BLAST 2 sequences, was used to compare each contig or BAC clone sequence to the most 5′ approximately 900 bases of the DNA sequence of U09116 (LRE2). The search revealed no full length high fidelity (98-100% identity to the 5′ L1 sequence) L1 elements in the whole of chromosome 21. However, the search did reveal three genes with full-length L1 elements in intronic gene segments: 1) APP, with an L1 element of 97% identity to the consensus sequence; 2) TTC3, with an L1 element of 90% identity to the 5′ L1 sequence; and 3) DSCAM, which includes two intronic L1 sequences, one with 93% and one with 87% identity to the 5′ L1 sequence (FIG. 4 and Table 5). Thus this search successfully identified APP, encoding amyloid precursor protein, as a candidate disease gene. Abundant data has linked the APP gene or altered regulation of the gene or protein product in Alzheimer disease. The search also identified two additional potential disease genes that might be relevant to other disease situations. While little information is available regarding TTC3, DSCAM is the gene encoding Down's syndrome cell adhesion molecule, a protein that has been implicated in Down's syndrome.

Example 9 Search for Genes and Gene Products Involved in Schizophrenia

[0181] Another example of the method of the invention begins with five chromosome loci defined by microsatellite markers identified in a screen of thirteen large families with schizophrenia (84). For each of the five markers, their location in a particular contig was identified by searching the NCBI nucleotide database against the microsatellite marker. For each marker, as list of contigs approximately 5 million bases on either side of the marker was generated. Each of the contigs was then searched against the most 5′ approximately 900 bases of the consensus L1 sequence U09116. The five lists of contigs, and the results of the search, are shown in Table 2.

[0182] Candidate disease-related genes could be identified for further testing. Of these, several appear to be particularly attractive candidates for involvement in a disease of the central nervous system, such as schizophrenia. This example is highly applicable to developing a series of candidate disease genes for any disease in which preliminary studies have generated credible susceptibility loci.

Example 11 Search for Genes and Gene Products Involved in SLE

[0183] This Example demonstrates a similar approach for a disease in which many loci with borderline statistical significance have been proposed to possibly identify disease genes. Total genome screens using microsatellite analysis of DNA from patients with SLE and their family members have been published. One of these studies was used to guide a study of several chromosomes rich in peaks with increased LOD score for linkage with SLE (4). Chromosome 1q had numerous peaks of increased LOD score; chromosome 16 had one major broad peak of increased LOD score; and chromosome 21 had a region of modestly increased LOD score. For each of these chromosomes, all contigs were listed and searched against the 5′ most 900 bases of U09116. Full-length high fidelity L1 sequences within 100,000 bases of a known or predicted gene and full-length L1 sequences included within introns or untranslated regions of known or predicted genes were identified.

[0184] The location of these elements were then displayed based on their location on the chromosome, with multiple L1 elements within a single gene identified by stacked bars (FIGS. 2, 3, and 4. The curves generated from the LOD scores of microsatellite markers studied in the Gaffney SLE study (4) were then freely drawn over the display of the identified L1 elements along chromosomes 1q, 16, and 21. Strikingly, the LOD curves closely follow the location of full-length high fidelity L1 elements and L1 elements within genes. These genes and their mRNA and protein products become candidates for further study of their disease relevance. In addition, the high fidelity L1 elements themselves may represent disease susceptibility genes, with their products contributing to the immune system activation characteristic of SLE.

[0185] The present invention is not to be limited in scope by the specific embodiments described herein. Indeed, various modifications of the invention in addition to those described herein will become apparent to those skilled in the art from the foregoing description and the accompanying figures. Such modifications are intended to fall within the scope of the appended claims. It is further to be understood that values are approximate, and are provided for description.

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[0276] 91. Gray-McGuire C, Moser K L, Gaffney P M, Kelly J, Yu H, Olson J M, Jedrey C M, Jacobs K B, Kimberly R P, Neas B R, Rich S S, Behrens T W, Harley J B. Genome scan of human systemic lupus erythematosus by regression modeling: evidence of linkage and epistasis at 4p16-15.2. Am J Hum Genet 67:1460-1469, 2000. TABLE 1 Chromosomal location of proposed SLE disease loci and full length high fidelity L1 elements. % sequence identity was determined in comparison to nt 1-884 of accession no. U09116. Location of Distance of Nearest 98 L1 from M Bases or 99% Marker (in Chromosome Marker From ptel identity L1 Gene containing L1 M bases) 1q FCGR2A 162.62 98 (167.4) none 4.78 1q D1S1679 163.021 98 (167.4) none 4.34 1q D1S213 182.58 98 (178.54) LOC127055 4.04 98 (182.4) none 0.18 98 (182.6) none 0.02 1q LAMC1 187.8 99 (185) XPR1 2.8 98 (186.7) None 1.1 98 (189.8) NIBAN 0.02 1q D1S2785 247 99 (244.3) RYR2 2.7 98 (244.3) LOC128172 2.7 98 (248.7) none 1.7 4p D4S403 28 98 (28) none 0 98 (31) none 3 4q D4S2368 168.5 98 (166.2) Similar to testican 2.3 99 (169.2) NEK1 0.7 6p D6S2410 53.57 98 (47.72) SUPT3H 5.58 99 (48.8) none 4.77 16q D16S415 54.31 98 (54.6) MGC5149 0.29 D16S503 65.28 98 (67.7) none 2.42 99 (70.5) none 5.22

[0277] TABLE 2 Analysis of L1 element expression in the genomic regions neighboring schizophrenia disease loci. Seven loc on 5 chromosomes were studied and are darkly highlighted. L1 % density Location to 1-884 of Marker U09116 (M bases location on Description or Additional from ptel) Contig contig Gene mRNA Description 162.7 NT_004406 91cds LOC127384 XM_060457 862,690 Sim to serum 88,85 amyloid P/crp family 163.7 NT_026945 88cds L0C126821 XM_060193 16.919 Dead/deah box helicase 165.4 NT_004745 97 D1s1679 89cds ATF6 2,404,741 88,87,87 87cds DDR2 1,349,888 167.1 NT_004648 95,90,90,87 88cds 1,489,481 ALDH9A1 D1S196 NT_004668 98 Near Sim to Ewings Z16503 AL031733 4,217,155 FLJ00024? sarcoma oncogene 170.1 (2,860,000) 97,95 LOC92233 (2,830,998) LOC127890 XM_059191 95cds LOC127910 XM_060726 6,071,165 94 Nucleoside 94cds NME7 diphosphate 1,194759 kinase 94cds LOC127914 XM_072920 6,774,236 92 91cds NME7 1,172,510 90 90cds LOC127911 XM_072918 6,314,453 89cds ATF6 CAMP-dep tran 9,220,139 factor 88,87,87 86cds LOC127891 XM_059193 4,403,629 84cds LOC127889 XM_072913 4,149,207 85 83cds LOC127906 XM_127906 5,501,845 90cds LOC57821 NM_021179 1,047,463 87cds LOC127934 XM_060744 8,510,019 jk 87cds Discoidin domain 7,810,544 DDR2 rec fam 2 Neurotrophic tyr kinase rec rel3 88cds 4,815,922 ALDH9A1 89cds 3,680,941 LOC127885 XM_060709 85cds 9,359,548 LOC127944 XM_060702 Near fcfr2b Rib prot L31 85cds 1,467,216 LOC127874 XM_072909 86cds 6,335,176 LOC127911 XM_072918 79cds 6,030,189 LOC127910 XM_060726 83cds 6,578,251 LOC127912 XM_060727 86cds Sim to adenyl cyc 6,540,496 LOC127912 81 cds 1,040,525 LOC57821 XM_001574 81cds 399,716 KIFAP3 Kinensin assoc protein 3 Sing GDS-assoc prot SMAP - cerebellum 174.7 NT_029874 97cds LOC127090 XM_060336 Ganglioside gm2 286,649 Shingolipid activator precursor activator prot 3 protein 3 96cds LOC127105 XM_072723 1,900,362 95cds LOC127100 XM_044463 968,927 94 93cds LOC127100 1,227,344 89 87cds LOC127109 XM_060332 2,235,511 Sum to rib prot L26 87cds LOC127109 2,140,534 86cds C1ORF9 XM_002138 1,510,476 90cds LOC92346 XM_044465 1,344,710 88cds LOC127091 XM_043678 469,620 KIAA1096 87cds LOC127100 1,215,001 81cds LOC127100 994,144 79cds LOC127100 937,597 84cds LOC127109 2,231,864 176 NT_021942 81 176.6 NT_029873 98 Near XM_031117 Phosphotyr 4281 LOC90354 Sim to Rab6 Binding dom 97cds LOC90354 GTPase act 216,844 protein 96,86 177.6 NT_004470 88 178 NT_029868 98cds LOC127055 XM_060298 Ribosomal L13e 302,955 like 95cds FLJ10416 Sim to 46,079 photomorphogenic 93 prot 92cds ″ 30,009 78cds LOC127056 XM_072714 479,243 179 NT_004398 bit 112.1 NT_028147 98cds LOC132718 XM_076833 2,142,361 96cds LOC132709 XM_067997 Kruppel-assoc box 563,171 FLJ10891 95 94cds LOC132712 XM_076837 1,036.908 94 93cds LOC132714 XM_067992 1,520,639 93,92 90cds LOC132715 XM_076830 1,621.371 90cds LOC132715 1,596.843 89 GNS1_SUR4 87cds LCE XM_045523 Long chain fatty 228,178 Acyl elongase 86cds LOC132707 XM_067995 479,677 Sim to Ac- Liketrans e1 84cds LOC132717 XM_076832 1.928,148 93cds LOC132714 XM_067992 1.431,709 87cds LOC132713 XM_067998 1.260,497 87cds EGF XM_003608 Mitogenic; stim 91.217 phos of H3; LDL rec domain Protect from LDL oxidation 78cds LOC132717 XM_076832 1.864,383 113.9 NT_006371 94cds CAMK2D Calcium/calmoduli 574.774 n Dep kinase delta2 May be involved in 92cds LOC133294 XM_077029-x neuron signaling 517,127 hippo/pyramidal cells 114.8 NT_022790 94 87cds LOC132392 XM_067820 649.533 86 D4S430 NT_029273 92,90,86 Z17169 AC053545 115.4 (111,507) 116 NT_028173 94cds LOC132747 XM_076846 45,911 116.2 NT_022931 Nss 116.7 NT_022989 87.88 83cds (17) 117.4 NT_016599 97 160.7 NT_023152 98 Near XM_077106 1.764,039 LOC133516 98 2.130,593 98cds(11 LOC133511 XM_068388 446,551 Sim pdgf rec like 97,97 Prot 94cds LOC133515 XM_077105 1.473,285 94 92cds LOC133514 XM_077104 1,275,463 92cds ATP10B ATPase 258,166 8887 87cds LOC133515 XM_077105 1,399,629 87 82cds GLRA1 Glycine rec alpha1 Mutated in hyper- 1,884,591 Startle dis/stiff Ekplexia (startle man syndrome dis) - exon 6 81cds LOC133512 XM_077102 530,876 80cds ATP10B 250,195 81cds LOC133515 1,454,062 163 NT_006788 98 Near XM_068801 1,682,752 LOC134347 86cds SGCD Sarcoglycan Knock out has 1,106,028 Dystrophin assoc cardiomyopathy gp 85cds LOC134344 XM_056963 377,215 88cds SGCD 1,135,192 86cds LOC134344 370,363 164.5 NT_029980 Nss 165.4 NT_007006 85,88,92,81 166.5 NT_029289 Nss 167 NT_023255 Nss 167.3 NT_025716 94 D5s422 NT_006983 94cds LOC134485 Z16965 AC010602 184,438 (167.97) (438,043) 91 (380,023) 87cds LOC134487 DSS400 349,741 D5S410 86cds LOC134491 807,389 82cds LOC134491 849,502 86cds LOC 134488 Sim to fibulin Ig domain 619,246 84cds LOC134486 278,288 172 NT_006907 88,87,87,85 175.5 NT_023154 96 87cds LOC133532 XM_077110 1,084,950 89cds LOC133527 XM_068392 596,953 84cds LOC133530 XM_077109 955,499 75cds LOC133527 675,271 0 NT_008060 86,90 0.7 NT_023779 91 1.5 NT_023744 97cds(31- LOC137257 XM_051541 Interacts with Hom 463,266 DLGAP2 of Drosophila discs Concentrated in tumor supp prot are synaptic junctions; assoc with NMDA Coprecip with rec and K channel GRIN2A prot; brain and (NMDAR2), kidney; cand for APC, and beta- progressive catenin; colocal epilepsy with with DLG4 and mental retardation GRIN2A in hippo 2.2 NT_008087 94,93 85cds LOC137745 XM_070579 461,662 Sim to Pto kinase 81cds (9- ″ interactor 448,734 3.8 NT_008227 94cds LOC137940 XM_078497 56,458 86cds CSMD1 XM_054838 CUB (found in C1r, 1,825,757 C1s, uEGF, and 85cds ″ BMP) and sushi 1,786,802 multiple domains 1; 87cds ″ CCP domain 1,625,088 (abundant in 94 complement control 94cds ″ proteins) 1,805,349 84cds LOC137946 XM_078501 342,566 5.6 NT_023736 88,87 6.3 NT_023904 Nss D8S503 NT_019483 98cds LOC137062 XM_078189 Z23470 2,224,912 7.28 86,86 (2,564,262) 85cds LOC137065 XM_070213 2,492,759 92 10 NT_023868 Nss 10.2 NT_030035 86 10.4 NT_026362 85 10.8 NT_008010 85 11.2 NT_008175 Nss 11.6 NT_008128 92 12.1 NT_008004 90cds FDFT1 XM_035680 Farnesyl- 93,373 Cholesterol diphosphate biosynthesis farnesyltransferase Rec syndrome 1 12.4 NT_029349 Nss 13 NT_008161 90cds LOC137868 XM_059926 Sim to zeta 1,633,926 sarcoglycan 13.6 NT_030030 Nss 21.9 NT_023704 84cds 141,075 86 22.4 NT_008205 77 22.9 NT_008300 93cds 319,091 23.3 NT_008047 Nss 23.8 NT_023664 Nss 24.6 NT_008130 95cds LOC137815 x 680,765 D8S1771 NT_023666 91,93 Z53561 91cds LOC137104 26.48 1,277,844 (517,449) 97cds LOC137097 361,474 86 27.3 NT_030023 98cds BNIP3L BCL2/adenovirus Mitochondria; 332,957 E1B 19kD- colacalizes with 60 interacting protein kD hsp-1; 3-like family proapoptotic fin; propoptotic binds BCL2 family members 27.9 NT_008139 99cds LOC137822 XM_070621 ? 611,028 87,87,86 29.5 NT_007988 93 31 NT_007993 85cds WRN Werner syndrome Scleroderma-like 185,359 RECQL2 Homolog of RecQ skin changes; helicase; 3′ to 5′ subcutaneous exonuclease; calcification; Nucleolar; homol premature gene in C. elegans arterioscl, DM; implicated in acceleration of silencing of telomere-driven transposons and replicative RNA interference senescence 101-125 NT_009151 125 NT_030107 Nss 129 NT_009215 94cds TECTA Tectorin alpha deafness 1,920,753 88 81cds GRIK4 Glutamate rec 2,324,593 99cds(118- LOC 120493 Sim to LINE RT XP_062069 1,215.303 homolog D11S934 NT_009115 98 Near PIG8 P53-induced gene Z17119 827,840 784,102 8; activates an 132.8 apoptotic pathway (1,384,127) LOC120253 Sim to surf gp, Ig D11s925 fam 98 494,488 FEZ1 Fasc and elong Axonal outgrowth 446,059 Protein zeta 1 Near LOC120251 527,380 Sim to etoposide- induced mRNA ITM1 550,705 Integral mem prot CHEK1 584,640 checkpoint monitors meiotic recombination 97cds LOC120271 XM_073548 3.036,022 94cds LOC120267 XM_073545 2,758,023 93,93,92,88 83,83,83 84cds LOC120293 4,978,908 80cds LOC120289 4,773.040 82cds LOC120253 880,521 96cds LOC120279 3,694,083 96cds LOC120275 3,364,553 138.3 NT_009056 93cds LOC120214 XM_07527 1,156,972 140.3 NT_024213 nss 141 NT_009276 95,85 142 NT_024180 bits D20S112 NT_011387 99 Near 23,329,380 17.25M AL031675 23,311,773 LOC128817 99 Near 12,701,572 12,692,681 LOC128729 99cds sim to 11,518,452 PGAM-B phosphoglycerate mutase, brain 97,96,96,96 form 95cds LOC128685 6,207,479 95 94cds LOC128795 21,802,506 94,94 99 near 6,805,558 7,007,833 LOC128691 93cds LOC128726 12,015341 94,92,91 89cds LOC128759 18,218,585 88cds LOC128812 22,879,343 87,87 84cds LOC128749 16,679,616 90cds LOC128753 17,006,560 88cds LOC128727 12,377,291 88cds LOC128649 2,150,115 88cds FLJ20212? 13,478,906 88cds Hi in islets of Lang 17,296,781 LOC96688 Sim to (17.15- neuroendocrine 17.4)XM_(—) convertase 2 046457 precursor (NEC 2) 87cds LOC128794 21,739,101 86cds LOC128666 4,287,601 Sim to SEL1L Suppressor of lin-12 87cds DJ842G6.2 XM_046437 Pancreas; negative 13,357,375 Regulator of notch 84cds LOC128714 pathway 11,038,855

[0278] TABLE 3 Contigs on chromosome 1q containing full-length L1 element sequences. Nss signifies no sequence homology to nt 1-884 of accession no. U09116. % identity to U09116 1-883 Chrom. M Bases Location of L1 Gene or Description of Gene Additional Location From ptel Contig in contig LocusLink Or similar Gene Description 1q 148.2 NT_022052? 89 NT_004434+ 95cds LOC127469 Sim to 435,546 (148.75) bM332P19.3 - 94,89 novel 7 88cds as above transmembrane 414,715 receptor rhodopsin 82cds LOC127475 family olfactory rec 874,365 (149.2) like protein 82cds as above 867,310 NT_030568 88 150 NT_029226− 89cds Cezanne Cellular zinc finger 737,990 (149.8) anti-NF kappa B- A20-like zinc finger domain (inhib of cell death) NT_004811 Bitcds NT_021907+ 89cds LOC126632 787,136 NT_004441 82,87 NT_030577? Nss 154.5 NT_021933 Nss NT_004524 Nss 1q22 NT_004858+ 85cds LOC128249 Sim to cell surface XM_060902 2,260,795 (157.95) molecule Ly-9 (Ly-9 = 94cds ″ (CD229) AF244129; 2,352,293 -Ig superfamily 29% identical) 87cds ″ (others are CD2, 2,322,154 CD48, CD58) 84cds ″ 2,355,589 84cds 2,300,855 NT_019291− Bits 159 NT_004982+ 94cds LOC128375 Sim to gamma 1,245,500 (159.83) interferon inducible 93,88,85,85.84 protein 16 NT_030566− 94 NT_026222? Nss 161 NT_004406− 91cds LOC127384 Sim to porcine Binds to lipid of 862,690 (160.62) Amyloid A protein apoptotic cells 88,85 NT_026945+ 88cds LOC126821 16,919 D1S1679 163.021 NT_004668 162-171 NT_004668− 98(167.4) Near Sim to Ewings Breakpoint 4,217,155 FLJ00024? sarcoma oncogene region 97,95 LOC92233 LOC127890 XM_059191 −95cds LOC127910 XM_060726 No con dom; 6,071,165 drohom 94 Nucleoside 94cds NME7 diphosphate kinase 1,194759 (170.2-.5) −94cds LOC127914 XM_072920 6,774,236 (964.85) 92 91cds NME7 1,172,510 (170.5 90 −90cds LOC127911 XM)072918 6,314,453 −89cds ATF6 CAMP-dep tran Unfolded prot 9,220,139 (162.3-.4) factor; binds SRF response 88,87,87 FLJ21522 −86cds LOC127891 XM_059193 Sim FLJ00024 4,403,629 (167.2) SH3 dom Phosphotyr Interaction Sim transporter 84cds LOC127889 XM_072913 4,149,207 85 −83cds LOC 127906 XM_127906 5,501,845 −0cds LOC57821 NM_021179 1,047,463 −87cds LOC 127934 XM_060744 8,510,019 87cds Discoidin domain Extracell factor 7,810,544 DDR2 rec fam 2 VIII-like (163.8) Neurotrophic tyr domain; act by kinase tee rel3 collagen 88cds 4,815,922 ALDH9A1 Catal dehydrogen- −89cds (166.75) Ation of GABA 3,680,941 LOC127885 XM_060709 Sim toFlavin cont −85cds LOC127944 XM_060702 Monooxygen5 9,359,548 Near fcfr2b Rib prot L31 (163.22) −85cds LOC127874 XM_072909 1,467,216 −86cds LOC127911 XM_072918 6,335,176 (165.4) −79cds LOC127910 XM_060726 6,030,189 (165.5) −3cds? LOC127912 XM_060727 6,578,251 (165.05) Sim to adenyl cyc −86cds? LOC127912 6,540,496 −81cds LOC57821 XM_001574 1,040,525 81cds KIFAP3 Kinensin assoc 399,716 (171.08- protein 3 171.2) Sing GDS-assoc Armadillo prot SMAP - repeats; phos by cerebellum v-src D1S196 168.76 NT_004732− 87,87,89,87 NT_030564− 87cds LOC127144 201,283 (174.1) 175.5 NT_029874+ 97cds LOC127090 Sim to ganglioside Ganglioside 286,649 (164.57) GM2 activator gm2 activator precursor (SAP-2) precursor protein 3 96cds LOC127105 XM_072723 1,900,362 95cds LOC127100 XM_044463 XP_044463 969,927 KIAA0820 94 (175.0) Dynamin 93cds LOC127100 domain GTPase 1,227,344 effector domain 89 (mediate vesicle 87cds LOC127109 XM_060332 trafficing); 2,235,511 Sim to rib prot L26 pleckstrin homo 87cds LOC127109 2,140,534 86cds C1ORF9 XM_002138 1.510,476 90cds LOC92346 XM_044465 1,344,710 88cds LOC127091 XM_043678 469,620 KIAA1096 87cds LOC127100 1,215,001 81cds LOC127100 994,144 79cds LOC127100 937,597 (175.6) 84cds LOC127109 2,231,864 NT_021942? Bits NT_030570? Nss 177.5 NT_004470− Bits NT_029868+ 98cds LOC127055 302,955 (178.54) 95cds FLJ10416 Sim to 46,079 (178.3) photomorph- 93 Ogenic prot 92cds FLJ10416 Sim to 30,009 photomorph- 78cds LOC127056 Ogenic prot 479,243 D1S1589 NT_026949+ 98(182.4) (182.58) 3,396,062 98cds (182.6) ? 3,597,400 97 97cds LOC90354 3,809,963 96cds As above 3,954,485 96 95cds LOC126850 1,184,773 94 93cds(31 LOC126858 2,588,833 91,90 88cds LOC126850 1,228,754 86,86 83cds FLJ10244 Guanine nuc Like SOS 2,432,829 exchange factor 83cds LOC126850 1,243,379 89cds NPHS2 podocin Neplirotic 3,170,271 (181.85) syndrome 185 NT_004552+ 99cds XPR1 Xenotropic and 1,317,693 (184.28) polytropic 87 retrovirus receptor 86cds LOC127663 ??? 2,407,307 (185.7) 86cds 2,445,258 as above 79cds 2,487,143 as above 88cds MGC2404 778,461 (183.93) NT_029219+ 98(186.7) Near Sim to embigin 85,973 LOC126918 96,92 (186.7) 96cds LOC126920 Sim to KIAA0456 379,853 (187.15) NT_029880? Nss LAMC1 188.6 NT_029864− 93cds LOC127028 (187.8) 983,033 (188.4) 89,89 78cds as above 956,095 NT_004487+ 98cds (189.8) C1orf24 Niban 340,228 98 Near 3,128,788 LOC127523 98 Near 2,880,876 LOC127522 And LOC 127521 95cds C1orf26 752,376 95,95,93,92 91cds FIBL-6 Sim to basement 1,658,122 (190.65) membrane specific heparan sulfate PG core protein precursor 92cds LOC127514 879,310 (190.3) 85cds LOC127519 Near PRG4 1,757,301 NT_021972− 86cdsbit 272,0623 195 NT_021905− 93,91,90 NT_004671+ 98cds LOC127964 Sim to ribosomal 2,492,213 protL23A 97 96cds LOC127946 -sim to myomegalin 6924 96 200 NT_004599+ 84,82 NT_021909+ Bits NT_004416− 99 Near Sim to H factor 1 - Also near factor 392,676 LOC127387 complement H (202.65) Isoform 1 -sim to complement 93cds LOC127388 factor H-rel proteiin 55 1,372 (202.5) 3 precursor-FUR- sushi 3 NT_029862+ 98 Near 797,582 LOC127012 93 (204.1) 93cds LOC127012 931,447 88cds FHRS Factor H-rel 83,139 (203.22) Prot 5 83cds 255,648 92cds LOC127012 958,777 96cds F13B Coag factor13B 134,515 (203.28) NT_030560 88 82cds LOC127117 258,938 207.5 NT_004680+ 92,92,89,86 D1S1678 NT_004662− 93cds LOC127827 Sim to gp330 676,800 (212.45) 92cds LOC93273 Sim to 481,841 (212.48) thymopoietin 83cds(57 LOC127827 534,300 NT_021924? 88 214 NT_029217− 89,88,90 IL-10, DAF area NT_030585+ 94,92 87cds LOC 127235 578,468 (214.92) 86cds(757- CR1 Small fragment 391,332 NT_030579? Nss NT_030575? Nss 216 NT_021877+ 98cds LOC126615 1,352.011 (217) 92 88cds as above 1,411.541 NT_030578− 90cds KCNH1 K voltage-gated Myoblasts at 1,301,974 (217.84- channel fusion stage 218.23) 89cds KCNH1 1,294.365 87 87cds MGC14801 731,351 (218.52) 86 90cds LOC127190 Sim to p21-Arc 686,368 88cds KCNH1 1,381,704 88cds KCNH1 1,265,982 80cds LOC127192 1,494,931 NT_004993+ 87cds LOC128385 694,358 87,85 85cds FLJ10874 378.593 (219.7) 87cds LOC128391 1,417,141 NT_029884? Nss 222 NT_004612− 93 87cds LOC127758 1,675,483 (221.25) 89cds as above 1,674,521 87cds LOC127742 577,128 87cds LOC127758 1,593,865 NT_030582+ Bits 1q41 225 NT_004817 98 Near 773,814 LOC128150 And LOC128149 96cds LOC128153 1,098,009 94 88cds FLJ10252 Glycine rich RNA 1,364,541 (225.25) nucleic acid processing binding domain protein? 79cds LOC128155 1,422,107 NT_029863+ Nss NT_029871+ 96cds RAB3- GTPase activating 739,776 GAP150 protein (228.1) 93cds LOC127083 319,420 93cds As above 295,189 1q42 229 NT_004642− 86 87cds LOC127803 146,862 84cds LOC127805 306,312 NT_029866+ 86cds LOC127046 42,308 NT_030576? Nss 230 NT_021953+ 85cds LOC126708 289,839 NT_029858? Nss 231 NT_004861+ 97 93cds LOC128253 348,708 93 85cds FLJ10052 Contains sushi 179,795 (231.6) domain; sim to 92 DAF precursor NT_004525− 83,83,93 ADPRTarea 83cds LOC127586 Sim to synapsin I (233.45) 313,463bit (234.43) 235 NT_004908+ 92,87 LOC128303 85cds 42,367 NT_004559+ Bits 237 NT_021973− bits 238.5 NT_004753− 91cds DISC1 Disrupted in Multiple 543,853 (238.61- schizophrenia sclerosis lesions 239.13) D1S3462 238.9 NT_004753 In DISC1 240 NT_004433− 96cds(757 LOC127452 10,613 NT_030561− 90,88 NT_022107? Nss D1S235 242.97 NT_004836 In CHS1 243.07 RYR2 = 244 NT_004836− 99cds RYR2 Cardiac Calcium release 244.3- 2,329,412 (244.3 Channel of 244.9 98cds sarcoplasmic 2,379197 LOC128172 reticulum D1S235 92,92 88cds Increased in kidney 3,235,442 TM7SF1 transmembrane cdspartial (243.38) D1S2785 248 NT_004771+ 98 Near Sim to pentraxin rel (248.7) LOC114922 gene-3 1,913,492 92 RGS7 GTPase activating Mostly in brain 91cds (147.52) 771,848 250 NT_004734+ 97,87,87 86cds AKT3 v-akt murine Protein kinase 2,159,228 (251.3-.4) thymoma viral B, gamma; oncogene pleckstrin homolog3 homol domain; 88cds LOC128041 Ser/thr kinase 870,185 (250.2) NT_026947? Nss NT_030586? Bits 254 NT_004536+ 99(252.6) Near Sim to olf recs 340,220 LOC127615 (309,963) And LOC 127616 (354,490) 97 95cds FLJ21080 SET domain; 1,954,493 (254.2) MYND finger 86 Sim to olfrec 85cds LOC127610 453,782 85cds LOC127622 546,320 256.4 NT_029870+ 97,92,92 Sim to olfactory rec 94cds LOC127057 1-25 21,328

[0279] TABLE 4 Location of full-length L1 elements on chromosome 16. Nss signifies no significant homology with nt 1-884 of L1 consensus sequence in accession no. U09116. L1 element % identical to nt 1-884 M Bases of consensus from Location ptel Marker Gene Contig in contig NT_010552 88 NT_010540 Nss NT_010388 Nss NT_027184 Nss NT_010543 Nss NT_015360 Nss 6.0 M NT_027178 Bit NT_010384 Nss NT_010537 84 11 M NT_010530 98 1,112,885 87 FLJ12668 87cds 1,997,396 12.5 M NT_010419 86 FLJ12363 92cds 571,065 86, 83 Myosin NT_010393 86, 86, 89, 89 NT_024760 Bit 17.3 M LOC115995 NT_024822 99, 3′ utr Sim to (208- LIP 25,588 isoform 91, 87 of BLIP NT_010584 Nss 20 M NT_024776 Nss NT_030153 95 NT_010436 Nss NT_024801 88 NT_010592 Nss NT_027182 88, 87 NT_010604 89, 88 27.5 M NT_010591 Nss NT_010441 88 NT_027176 Nss NT_010589 Nss NT_024802 Nss 32 M ITGAM NT_024812 88cds (CR3:CD11b) 447,353 44.3 M NT_028368 89 45 M NT_024773 98 671,001 98 748,545 93, 92, 92, 90, 90 88, 87 45.5 M NT_010570 96, 92, PHKB 90cds 564,374 89 CDA08 84cds 900,968 91 CDA08 91cds 1,065,272 CDA08 86cds 810,315 88, 85 PHKB 82cds 700,819 CDA08 87cds 822,483 PHKB 85cds 693,085 NT_010637 Nss NT_010505 97, 84, 87 50 M Near LOC NT_024779 98 115613 811,937 sim to Na 94, 91, 88 and Cl-dep 89cds transpo 596,749 ZNF267 NT_029461 Nss NT_010493 Nss NT_010521 86, 85, 83 54 M D16S MGC5149 NT_010498 98cds 419 746,473 D16S415 85, 96, 91 55.8 M AMFR NT_019610 93, 93 Autocrine 89cds motility 288,196 factor 57.4 M NT_024766 bit 58.6 M NT_010406 94, 83 60.2 M NT_029457 95, 95, 93, 86, 86 62.5 M CDH8x3 NT_010463 97cds Cadherin 8 CDH8x3 1,670,319 96cds 1,689,733 95cds 1,806583 93, 93, 89 62.9 NT_019621 Nss 63.5 NT_010615 93, 92, 91 65 (77) D16S3253 0.1/3.2 NT_010558 95, 87 D16S503 66.6 NT_010546 98 758,900 92, 92 69.8 Near CDH3 NT_010478 99 (placental) 2,329,912 88 71.1 NT_019608 Nss 71.9 NT_030154 Nss 72.1 NT_028369 bit 72.6 Near DKFZp NT_010635 99 434L0850 6635 73.1 NT_010580 Nss 73.4 NT_027175 88 74.2 NT_024792 97, 86 NT_029456 Nss 76 M Near NT_010556 99 LOC93220 782,847 Sim to laminin rec 1 NT_030151 Nss NT_024793 84 78 M NT_010480 85 NT_030152 Nss NT_024797 Bits NT_026456 Nss NT_024827 87, 86 NT_024821 Nss NT_010380 93 NT_010422 97, 86, 80 NT_028372 Nss NT_010494 Bits NT_024814 96, 94, 90, 84 86.5 M CDH13 NT_010428 99cds (heart) 701,822 NT_028371 Nss NT_024767 Bit NT_024788 95, 94 NT_030156 Nss NT_019609 Nss NT_024772 Nss NT_024782 Nss NT_024759 Bit 90.8 M GALNSx2 NT_010404 91cds Galacto- 62,543 samine 91cds sulfatase 69,091 NT_010632 Nss 92 M NT_010542 Nss

[0280] TABLE 5 Location of full-length L1 elements on chromosome 21. Nss signifies no significant homology with nt 1-884 of L1 consensus sequence in accession no. U09116 L1 element % identity to Location consensus 1-884 M Bases Contig or location in from ptel BAC clone contig Gene 7.8 M NT_029490 80 APP NT_011512 97 AD1 22,448,995 11-39.6 M 97 1,963,309 97 20,880,812 23.9 M 97cds APP 12,869,179 96, 94, 93 93cds DSCAM 26,987,787 93, 91 90cds TTC3 24,057,658 89, 89, 88 87cds DSCAM 27,064,029 86, 86, 85 11 M AP001464 Nss AJ239321 89 AP001170 Nss AP001135 Nss AJ239318 Nss AL049911 Nss AL050302 Nss AL078475 Nss AP001465 Nss AL163204 86 AL109748 Nss AP001466 Nss AP001634 83 AP001347 89 AL078615 Nss AF130358 83 AF130249 Nss STCH AF130247 Nss 12.6 AF165138 Nss AF198098 88 AF130351 Nss AF127936 88 AF248484 97 86,680 87 AF127577 97 9772 87 AF222684 Nss AF222685 Nss 13.3 AF130248 Nss AF246928 Nss AJ006998 Nss AJ009632 Nss AJ006997 Nss AL034449 Nss AJ010597 Nss AJ010598 91 14 AL109762 91 AP001344 Nss AP001346 89 AP001343 80 AP001172 Nss AP000962 Nss 14.65 AP000473 Nss AF130359 76 AF212831 Nss AF130418 93, 90 AP001250 Nss AP000457 85, 93, 85, 86 AP000968 94 AP000952 96 AP000963 Nss 15.6 AP000967 Nss AP000432 Nss AL109761 80 AP000404 Nss AP000745 Nss AF165175 Nss 16 AP000998 Nss AF130417 Nss AP000656 Nss AL078474 Nss AP000456 Nss 16.45 AP000455 Nss AL109763 Nss AF240627 Nss AP001538 93 17 AL157359 Nss AP001345 Nss AP000431 Nss AP000433 Nss AP000855 Nss AP000958 Nss AP000566 83 AP000401 Nss AP000403 Nss AP000568 Nss AP000946 Nss 17.9 AF238375 Nss AP001256 Nss AP001171 Nss 18.3 AP001254 Nss D21S1437 AP001251 Nss 18.6 AP001506 Nss AL109772 Nss AP000957 Nss AP000947 Nss AL035532 Nss AP000460 83 19 AF135405 Nss AP001138 Nss AP001136 Nss AP001252 Nss AP001137 Nss AP001114 Nss AP001115 Nss AP001117 Nss AF241725 Nss AP000475 Nss AP000472 Nss AP000454 Nss 20.05 AP000951 Nss AP000705 82 AP000657 Nss AP000561 Nss AP000953 Nss AP000959 82 AP000966 Nss AP000955 Nss 20.9 AP000949 Nss AP001116 Nss AP001255 Nss AP001253 Nss AP000459 Nss AP000965 Nss AP000950 Nss AP000961 Nss AP000960 Nss AP000474 Nss AP000477 Nss 22.1 AP000470 Nss AP000469 Nss AP000458 Nss AP000954 Nss AP000476 Bit AP001079 Nss AP000964 Nss AP000948 Nss AP000402 Nss AP000146 Bit AP001342 Nss AP000235 Nss AP000234 Nss AP000233 Nss AP000232 Nss AP001341 Nss AP001340 Nss AP001348 Nss AP000220 Bits AP000221 Nss AL109616 Nss AP000223 Nss AP000224 Nss AP000225 Nss AP000226 Nss AP000227 Nss AP000228 Nss AP001443 Nss APP 23.9 M AP001442 97cds APP 7857 AP001440 Nss APP AP001441 Nss APP AP001439 bit APP AP000229 Nss AP000230 Nss AP001595 Nss AP001596 Nss — 33.9 AF020802 Nss AP000687 Nss AP000688 Nss AP000689 Nss AP000690 Nss AP000691 Nss AP000692 80 AP000693 Nss AP000694 Nss AP000695 Nss AP000696 86 34.6 AP000697 Nss AP000698 Nss AP000699 Nss AP000700 Nss AP000701 Nss AP000702 Nss AP000703 Nss AP001418 Nss AP000704 Nss AP001435 Nss AP001431 Nss AP001429 90 TTC3 35.1 AP001432 Nss TTC3 DSCR3 AP001412 Nss DSCR3 AP001437 Nss AP001428 Nss AP001413 Nss AP001421 Nss AP001414 Nss AP001419 Nss AP001407 Nss AP001436 Nss 35.7 AP001416 Nss AP001430 Nss AP001408 Nss AP001411 Nss AP001424 Nss AP001433 Nss AP001427 Nss AP001415 Nss AP001410 Nss AP001420 Nss AP001409 Nss AP001417 Nss AP001425 Nss AP001434 Nss 36.4 AP001438 Nss AP001422 Nss AP001423 Nss AP001426 Nss AP001035 Nss AP001036 Nss AP001037 Nss AP001038 Nss AP001039 Nss 36.72 AP001040 Nss AP001041 Nss AP001042 Nss AP001043 Nss AP001044 Nss AP001045 Nss AF064858 Nss AF064859 Nss AF129408 Nss 37.3 AF064861 Nss AF121781 Nss AF121897 Nss 37.6 AF064860 Bit AF121782 nss AF064857 Nss AF045449 Nss AF064862 93, 87, 96 DSCAM AF064865 87 ″ AF042091 Nss ″ 38.3 AF042090 Nss ″ AF064863 Nss AF165176 Nss AF064864 Nss AF064866 Nss AF043945 AL442166 Nss AL442167 Nss 39.5 AP001610 nss 39.6 M — Nss NT_030187 40.5 NT_030188 Bit 43 NT_011515 bit

[0281]

0 SEQUENCE LISTING <160> NUMBER OF SEQ ID NOS: 15 <210> SEQ ID NO 1 <211> LENGTH: 6539 <212> TYPE: DNA <213> ORGANISM: Homo sapiens <300> PUBLICATION INFORMATION: <308> DATABASE ACCESSION NUMBER: GenBank Accession No. U09116 <309> DATABASE ENTRY DATE: 1995-02-02 <313> RELEVANT RESIDUES: (1)..(6539) <400> SEQUENCE: 1 gaataggaac agctccggtc tacagctccc agcgtgagcg acgcagaaga cggtgatttc 60 tgcatttcca tctgaggtac cgggttcatc tcactaggga gtgccagaca gtgggcgcag 120 gccagtgtgt gtgcgcaccg tgcgcgagcc gaagcagggc gaggcattgc ctcacctggg 180 aagcgcaagg ggtcagggag ttccctttcc gagtcaaaga aaggggtgat ggacgcacct 240 ggaaaatcgg gtcactccca cccgaatatt gcgcttttca gaccggctta agaaacggcg 300 caccacgaga ctatatccca cacctggctc agagggtcct acgcccacgg aatctcgctg 360 attgctagca cagcagtctg agatcaaact gcaaggcggc aacgaggctg ggggaggggc 420 gcccgccatt gcccaggctt gcttaggtaa acaaagcagc cgggaagctc gaactgggtg 480 gagcccacca cagctcaagg aggcctgcct gcctctgtag gctccacctc tgggggcagg 540 gcacagacaa acaaaaaggc agcagtaacc tctgcagact taagtgtccc tgtctgacag 600 ctttgaagag agcagtggtt ctcccagcac gcagctggag atctgagaac gggcagactg 660 cctcctcaag tgggtccctg acccctgacc cccgagcagc ctaactggga ggcacccccc 720 agcaggggca cactgacacc tcacacggca gggtattcca acagacctgc agctgagggt 780 cctgtctgtt agaaggaaaa ctaacaacca gaaaggacat ctacaccgaa aacccatctg 840 tacatcacca tcatcaaaga ccaaaagtag ataaaaccac aaagatgggg aaaaaacaga 900 acagaaaaac tggaaactct aaaacgcaga gcgcctctcc tcctccaaag gaacgcagtt 960 cctcaccagc aacagaacaa agctggatgg agaatgattt tgacgagctg agagaagaag 1020 gcttcagacg atcaaattac tctgagctac gggaggacat tcaaaccaaa ggcaaagaag 1080 ttgaaaactt tgaaaaaaat ttagaagaat gtataactag aataaccaat acagagaagt 1140 gcttaaagga gctgatggag ctgaaaacca aggctcgaga actacgtgaa gaatgcagaa 1200 gcctcaggag ccgatgcgat caactggaag aaagggtatc agcaatggaa gatgaaatga 1260 atgaaatgaa gcgagaaggg aagtttagag aaaaaagaat aaaaagaaat gagcaaagcc 1320 tccaagaaat atgggactat gtgaaaagac caaatctacg tctgattggt gtacctgaaa 1380 gtgatgtgga gaatggaacc aagttggaaa acactctgca ggatattatc caggagaact 1440 tccccaatct agcaaggcag gccaacgttc agattcagga aatacagaga acgccacaaa 1500 gatactcctc gagaagagca actccaagac acataattgt cagattcacc aaagttgaaa 1560 tgaaggaaaa aatgttaagg gcagccagag agaaaggtcg ggttaccctc aaagggaagc 1620 ctatcagact aacagcagat ctctcggcag aaaccctaca agccagaaga gagtgggggc 1680 caatattcaa cattcttaaa gaaaagaatt ttcaacccag aatttcattt ccagccaaac 1740 taagcttcat aagtgaagga gaaagaaaat actttacaga caagcaaatg ctgagagatt 1800 ttgtcaccac caggcctacc ctaaaagagc tcctgaagga agcactaaac atggaaagga 1860 acaaccggta ccagccgctg caaaatcatg ccaaaatgta aagaccatcg agactaggaa 1920 gaaactgcat caactaatga gcaaaatcac cagctaacat cataatgaca ggatcaaatt 1980 cacacataac aatattaact ttaaatataa atggactaaa ttctgcaatt aaaagacaca 2040 gactggcaag ttggataaag agtcaagacc catcagtgtg ctgtattcag gaaacccatc 2100 tcatgtgcag agacacacat aggctcaaaa taaaaggatg gaggaagatc taccaagcaa 2160 atggaaaaca aaaaaaggca ggggttgcaa tcctagtctc tgataaaaca gactttaaac 2220 caacaaagat caaaagagac aaagaaggcc attacataat ggtaaaggga tcaattcaac 2280 aagaggagct aactatccta aatatttatg cacccaatac aggagcaccc agattcataa 2340 agcaagtcct gagtgaccta caaagagact tagactccca cacattaata atgggagact 2400 ttaacacccc actgtcaata ttagacagat caacgagaca gaaagtcaac aaggataccc 2460 aggaattgaa ctcagctctg caccaagcag acctaataga catctacaga actctccacc 2520 ccaaatcaac agaatataca tttttttcag caccacacca cacctattcc aaaatcgacc 2580 acatagttgg aagtaaagct ctcctcagca aatgtaaaag aacagaaatt ataacaaact 2640 atctctcaga ccacagtgca atcaaactag aactcaggat taagaatctc actcaaagcc 2700 gctcaactac atggaaactg aacaacctgc tcctgaatga ctactgggta cataacgaaa 2760 tgaaggcaga aataaagatg ttctttgaaa ccaacgagaa caaagacacc acataccaga 2820 atctctggga cgcattcaaa gcagtgtgta gagggaaatt tatagcacta aatgcctaca 2880 agagaaagca ggaaagatcc aaaattgaca ccctaacatc acaattaaaa gaactagaaa 2940 agcaagagca aacacattca aaagctagca gaaggcaaga aataactaaa atcagagcag 3000 aactgaagga aatagagaca caaaaaaccc ttcaaaaaat caatgaatcc aggagctggt 3060 tttttgaaag gatcaacaaa attgatagac cgctagcaag actaataaag aaaaaaagag 3120 agaagaatca aatagacaca ataaaaaatg ataaagggga tatcaccacc gatcccacag 3180 aaatacaaac taccatcaga gaatactaca aacacctcta cgcaaataaa ctagaaaatc 3240 tagaagaaat ggatacattc ctcgacacat acactctccc aagactaaaa caggaagaag 3300 ttgaatctct gaatggacca ataacaggct ctgaaattgt ggcaataatc aatagtttac 3360 caaccaaaaa gagtccagga ccagatggat tcacagccga attctaccag aggtacaagg 3420 aggaactggt accattcctt ctgaaactat tccaatcaat agaaaaagag ggaatcctcc 3480 ctaactcatt ttatgaggcc agcatcattc tgataccaaa gccgggcaga gacacaacca 3540 aaaaagagaa ttttagacca atatccttga tgaacattga tgcaaaaatc ctcaataaaa 3600 tactggcaaa ccgaatccag cagcacatca aaaagcttat ccaccatgat caagtgggct 3660 tcatccctgg gatgcaaggc tggttcaata tacgcaaatc aataaatgta atccagcata 3720 taaacagagc caaagacaaa aaccacatga ttatctcaat agatgcagaa aaagcctttg 3780 acaaaattca acaacccttc atgctaaaaa ctctcaataa attaggtatt gatgggacgt 3840 atttcaaaat aataagagct atctatgaca aacccacagc caatatcata ctgaatgggc 3900 aaaaactgga agcattccct ttgaaaactg gcacaagaca gggatgccct ctctcaccgc 3960 tcctattcaa catagtgttg gaagttctgg ccagggcaat caggcaggag aaggaaataa 4020 agggtattca attaggaaaa gaggaagtca aattgtccct gtttgcagac gacatgattg 4080 tttatctaga aaaccccatt gtctcagccc aaaatctcct taagctgata agcaacttca 4140 gcaaagtctc aggatacaaa atcaatgtac aaaaatcaca agcattctta tacaccaaca 4200 acagacaaac agagagccaa atcatgggtg aactcccatt cacaattgct tcaaagagga 4260 taaaatacct aggaatccaa cttacaaggg atgtgaagga cctcttcaag gagaactaca 4320 aaccactgct caaggaaata aaagaggaca caaacaaatg gaagaacatt ccatgctcat 4380 gggtaggaag aatcaatatc gtgaaaatgg ccatactgcc caaggtaatt tacagattca 4440 atgccatccc catcaagcta ccaatgactt tcttcacaga attggaaaaa actactttaa 4500 agttcatatg gaaccaaaaa agagcccgca ttgccaagtc aatcctaagc caaaagaaca 4560 aagctggagg catcacacta ccttacttca aactatacta caaggctaca gtaaccaaaa 4620 cagcatggta ctggtaccaa aacagagata tagatcaatg gaacagaaca gagccctcag 4680 aaataatgcc acatatctac aactatctga tctttgacaa acctgagaaa aacaagcaat 4740 ggggaaagga ttccctattt aataaatggt gctgggaaaa ctggctagcc atatgtagaa 4800 agctgaaact ggatctcttc cttacacctt atacaaaaat caattcaaga tggattaaag 4860 atttaaacgt taaacctaaa accataaaaa ccctagaaga aaacctaggc attaccattc 4920 aggacatagg cgtgggcaag gacttcatgt ccaaaacacc aaaagcaatg gcaacaaaag 4980 acaaaattga caaatgggat ctaattaaac taaagagctt ctgcacagca aaagaaacta 5040 ccatcagagt gaacaggcaa cctacaacat gggagaaaat tttcgcaacc tactcatctg 5100 acaaagggct aatatccaga atctacaatg aactcaaaca aatttacaag aaaaaaacaa 5160 acaaccccat caaaaagtgg gcgaaggaca tgaacagaca cttctcaaaa gaagacattt 5220 atgcagccaa aaaacacatg aagaaatgct catcatcact ggccatcaga gaaatgcaaa 5280 tcaaaaccac tatgagatat catctcacac cagttagaat ggcaatcatt aaaaagtcag 5340 gaaacaacag gtgctggaga ggatgcggag aaataggaac acttttacac tgttggtggg 5400 actgtaaact agttcaacca ttgtggaagt cagtgtggcg attcctcagg gatctagaac 5460 tagaaatacc atttgaccca gccatcccat tactgggtat atacccagag gactataaat 5520 catgctgcta taaagacaca tgcactcgta tgtttattgc ggcactattc acaatagcaa 5580 aaacttggaa ccaacccaaa tgtccaacaa tgatagactg gattaagaaa atgtggcaca 5640 tatacaccat ggaatattat gcagccataa aaaatgatga gttcatatcc tttgtaggga 5700 catggatgaa attggaaacc atcattctca gtaaactatc gcaagaacaa aaaaccaaac 5760 accgcatatt ctcactcata ggtgggaatt gaacaatgag atcacatgga cacaggaagg 5820 ggaatatcac actctgggga ctgtggtggg gtcgggggag gggggagggg tagcattggg 5880 agatatacct aatgctagat gacacattag tgggtgcagc gcaccagcat ggcacatgta 5940 tacatatgta actaacctgc acaatgtgca catgtaccct aaaacttaga gtataattaa 6000 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagatca caccactgca ctccagcctg 6060 ggtgtcaaag cgagaccctg tctcaggaaa aaaaaaaaaa aaaaaaaaaa aggcttaatt 6120 gattgaacca gattcgagaa aacagtgcta aattataatt ttctcaatac tgtaaatatt 6180 tttcaatctt cagcttcatt aacttctata attgaaatta tcccaattat tacctgacat 6240 gtactaaaat tccctaaaat ggatcttgag taacattttc acagtacgat aatttttctc 6300 tctgtatata tttatatagt cacatatatg cacatacatt atacaagcat tacttttcta 6360 taactgtaag gtcagaattt gaagttgtgt tttctttatc tttttatttc caatacttgg 6420 catcaagttg atattcatta gaagtaaagg aggaaggaaa tgaataatct tcagatacta 6480 agaacattac acttaaatta ttattaaatc taatttgcat tctcatatat ggcttagct 6539 <210> SEQ ID NO 2 <211> LENGTH: 338 <212> TYPE: PRT <213> ORGANISM: Homo sapiens <300> PUBLICATION INFORMATION: <308> DATABASE ACCESSION NUMBER: GenBank Accession No. U09116 <309> DATABASE ENTRY DATE: 1995-02-02 <313> RELEVANT RESIDUES: (1)..(338) <400> SEQUENCE: 2 Met Gly Lys Lys Gln Asn Arg Lys Thr Gly Asn Ser Lys Thr Gln Ser 1 5 10 15 Ala Ser Pro Pro Pro Lys Glu Arg Ser Ser Ser Pro Ala Thr Glu Gln 20 25 30 Ser Trp Met Glu Asn Asp Phe Asp Glu Leu Arg Glu Glu Gly Phe Arg 35 40 45 Arg Ser Asn Tyr Ser Glu Leu Arg Glu Asp Ile Gln Thr Lys Gly Lys 50 55 60 Glu Val Glu Asn Phe Glu Lys Asn Leu Glu Glu Cys Ile Thr Arg Ile 65 70 75 80 Thr Asn Thr Glu Lys Cys Leu Lys Glu Leu Met Glu Leu Lys Thr Lys 85 90 95 Ala Arg Glu Leu Arg Glu Glu Cys Arg Ser Leu Arg Ser Arg Cys Asp 100 105 110 Gln Leu Glu Glu Arg Val Ser Ala Met Glu Asp Glu Met Asn Glu Met 115 120 125 Lys Arg Glu Gly Lys Phe Arg Glu Lys Arg Ile Lys Arg Asn Glu Gln 130 135 140 Ser Leu Gln Glu Ile Trp Asp Tyr Val Lys Arg Pro Asn Leu Arg Leu 145 150 155 160 Ile Gly Val Pro Glu Ser Asp Val Glu Asn Gly Thr Lys Leu Glu Asn 165 170 175 Thr Leu Gln Asp Ile Ile Gln Glu Asn Phe Pro Asn Leu Ala Arg Gln 180 185 190 Ala Asn Val Gln Ile Gln Glu Ile Gln Arg Thr Pro Gln Arg Tyr Ser 195 200 205 Ser Arg Arg Ala Thr Pro Arg His Ile Ile Val Arg Phe Thr Lys Val 210 215 220 Glu Met Lys Glu Lys Met Leu Arg Ala Ala Arg Glu Lys Gly Arg Val 225 230 235 240 Thr Leu Lys Gly Lys Pro Ile Arg Leu Thr Ala Asp Leu Ser Ala Glu 245 250 255 Thr Leu Gln Ala Arg Arg Glu Trp Gly Pro Ile Phe Asn Ile Leu Lys 260 265 270 Glu Lys Asn Phe Gln Pro Arg Ile Ser Phe Pro Ala Lys Leu Ser Phe 275 280 285 Ile Ser Glu Gly Glu Arg Lys Tyr Phe Thr Asp Lys Gln Met Leu Arg 290 295 300 Asp Phe Val Thr Thr Arg Pro Thr Leu Lys Glu Leu Leu Lys Glu Ala 305 310 315 320 Leu Asn Met Glu Arg Asn Asn Arg Tyr Gln Pro Leu Gln Asn His Ala 325 330 335 Lys Met <210> SEQ ID NO 3 <211> LENGTH: 1275 <212> TYPE: PRT <213> ORGANISM: Homo sapiens <300> PUBLICATION INFORMATION: <308> DATABASE ACCESSION NUMBER: GenBank Accession No. U09116 <309> DATABASE ENTRY DATE: 1995-02-02 <313> RELEVANT RESIDUES: (1)..(1275) <400> SEQUENCE: 3 Met Thr Gly Ser Asn Ser His Ile Thr Ile Leu Thr Leu Asn Ile Asn 1 5 10 15 Gly Leu Asn Ser Ala Ile Lys Arg His Arg Leu Ala Ser Trp Ile Lys 20 25 30 Ser Gln Asp Pro Ser Val Cys Cys Ile Gln Glu Thr His Leu Met Cys 35 40 45 Arg Asp Thr His Arg Leu Lys Ile Lys Gly Trp Arg Lys Ile Tyr Gln 50 55 60 Ala Asn Gly Lys Gln Lys Lys Ala Gly Val Ala Ile Leu Val Ser Asp 65 70 75 80 Lys Thr Asp Phe Lys Pro Thr Lys Ile Lys Arg Asp Lys Glu Gly His 85 90 95 Tyr Ile Met Val Lys Gly Ser Ile Gln Gln Glu Glu Leu Thr Ile Leu 100 105 110 Asn Ile Tyr Ala Pro Asn Thr Gly Ala Pro Arg Phe Ile Lys Gln Val 115 120 125 Leu Ser Asp Leu Gln Arg Asp Leu Asp Ser His Thr Leu Ile Met Gly 130 135 140 Asp Phe Asn Thr Pro Leu Ser Ile Leu Asp Arg Ser Thr Arg Gln Lys 145 150 155 160 Val Asn Lys Asp Thr Gln Glu Leu Asn Ser Ala Leu His Gln Ala Asp 165 170 175 Leu Ile Asp Ile Tyr Arg Thr Leu His Pro Lys Ser Thr Glu Tyr Thr 180 185 190 Phe Phe Ser Ala Pro His His Thr Tyr Ser Lys Ile Asp His Ile Val 195 200 205 Gly Ser Lys Ala Leu Leu Ser Lys Cys Lys Arg Thr Glu Ile Ile Thr 210 215 220 Asn Tyr Leu Ser Asp His Ser Ala Ile Lys Leu Glu Leu Arg Ile Lys 225 230 235 240 Asn Leu Thr Gln Ser Arg Ser Thr Thr Trp Lys Leu Asn Asn Leu Leu 245 250 255 Leu Asn Asp Tyr Trp Val His Asn Glu Met Lys Ala Glu Ile Lys Met 260 265 270 Phe Phe Glu Thr Asn Glu Asn Lys Asp Thr Thr Tyr Gln Asn Leu Trp 275 280 285 Asp Ala Phe Lys Ala Val Cys Arg Gly Lys Phe Ile Ala Leu Asn Ala 290 295 300 Tyr Lys Arg Lys Gln Glu Arg Ser Lys Ile Asp Thr Leu Thr Ser Gln 305 310 315 320 Leu Lys Glu Leu Glu Lys Gln Glu Gln Thr His Ser Lys Ala Ser Arg 325 330 335 Arg Gln Glu Ile Thr Lys Ile Arg Ala Glu Leu Lys Glu Ile Glu Thr 340 345 350 Gln Lys Thr Leu Gln Lys Ile Asn Glu Ser Arg Ser Trp Phe Phe Glu 355 360 365 Arg Ile Asn Lys Ile Asp Arg Pro Leu Ala Arg Leu Ile Lys Lys Lys 370 375 380 Arg Glu Lys Asn Gln Ile Asp Thr Ile Lys Asn Asp Lys Gly Asp Ile 385 390 395 400 Thr Thr Asp Pro Thr Glu Ile Gln Thr Thr Ile Arg Glu Tyr Tyr Lys 405 410 415 His Leu Tyr Ala Asn Lys Leu Glu Asn Leu Glu Glu Met Asp Thr Phe 420 425 430 Leu Asp Thr Tyr Thr Leu Pro Arg Leu Lys Gln Glu Glu Val Glu Ser 435 440 445 Leu Asn Gly Pro Ile Thr Gly Ser Glu Ile Val Ala Ile Ile Asn Ser 450 455 460 Leu Pro Thr Lys Lys Ser Pro Gly Pro Asp Gly Phe Thr Ala Glu Phe 465 470 475 480 Tyr Gln Arg Tyr Lys Glu Glu Leu Val Pro Phe Leu Leu Lys Leu Phe 485 490 495 Gln Ser Ile Glu Lys Glu Gly Ile Leu Pro Asn Ser Phe Tyr Glu Ala 500 505 510 Ser Ile Ile Leu Ile Pro Lys Pro Gly Arg Asp Thr Thr Lys Lys Glu 515 520 525 Asn Phe Arg Pro Ile Ser Leu Met Asn Ile Asp Ala Lys Ile Leu Asn 530 535 540 Lys Ile Leu Ala Asn Arg Ile Gln Gln His Ile Lys Lys Leu Ile His 545 550 555 560 His Asp Gln Val Gly Phe Ile Pro Gly Met Gln Gly Trp Phe Asn Ile 565 570 575 Arg Lys Ser Ile Asn Val Ile Gln His Ile Asn Arg Ala Lys Asp Lys 580 585 590 Asn His Met Ile Ile Ser Ile Asp Ala Glu Lys Ala Phe Asp Lys Ile 595 600 605 Gln Gln Pro Phe Met Leu Lys Thr Leu Asn Lys Leu Gly Ile Asp Gly 610 615 620 Thr Tyr Phe Lys Ile Ile Arg Ala Ile Tyr Asp Lys Pro Thr Ala Asn 625 630 635 640 Ile Ile Leu Asn Gly Gln Lys Leu Glu Ala Phe Pro Leu Lys Thr Gly 645 650 655 Thr Arg Gln Gly Cys Pro Leu Ser Pro Leu Leu Phe Asn Ile Val Leu 660 665 670 Glu Val Leu Ala Arg Ala Ile Arg Gln Glu Lys Glu Ile Lys Gly Ile 675 680 685 Gln Leu Gly Lys Glu Glu Val Lys Leu Ser Leu Phe Ala Asp Asp Met 690 695 700 Ile Val Tyr Leu Glu Asn Pro Ile Val Ser Ala Gln Asn Leu Leu Lys 705 710 715 720 Leu Ile Ser Asn Phe Ser Lys Val Ser Gly Tyr Lys Ile Asn Val Gln 725 730 735 Lys Ser Gln Ala Phe Leu Tyr Thr Asn Asn Arg Gln Thr Glu Ser Gln 740 745 750 Ile Met Gly Glu Leu Pro Phe Thr Ile Ala Ser Lys Arg Ile Lys Tyr 755 760 765 Leu Gly Ile Gln Leu Thr Arg Asp Val Lys Asp Leu Phe Lys Glu Asn 770 775 780 Tyr Lys Pro Leu Leu Lys Glu Ile Lys Glu Asp Thr Asn Lys Trp Lys 785 790 795 800 Asn Ile Pro Cys Ser Trp Val Gly Arg Ile Asn Ile Val Lys Met Ala 805 810 815 Ile Leu Pro Lys Val Ile Tyr Arg Phe Asn Ala Ile Pro Ile Lys Leu 820 825 830 Pro Met Thr Phe Phe Thr Glu Leu Glu Lys Thr Thr Leu Lys Phe Ile 835 840 845 Trp Asn Gln Lys Arg Ala Arg Ile Ala Lys Ser Ile Leu Ser Gln Lys 850 855 860 Asn Lys Ala Gly Gly Ile Thr Leu Pro Tyr Phe Lys Leu Tyr Tyr Lys 865 870 875 880 Ala Thr Val Thr Lys Thr Ala Trp Tyr Trp Tyr Gln Asn Arg Asp Ile 885 890 895 Asp Gln Trp Asn Arg Thr Glu Pro Ser Glu Ile Met Pro His Ile Tyr 900 905 910 Asn Tyr Leu Ile Phe Asp Lys Pro Glu Lys Asn Lys Gln Trp Gly Lys 915 920 925 Asp Ser Leu Phe Asn Lys Trp Cys Trp Glu Asn Trp Leu Ala Ile Cys 930 935 940 Arg Lys Leu Lys Leu Asp Leu Phe Leu Thr Pro Tyr Thr Lys Ile Asn 945 950 955 960 Ser Arg Trp Ile Lys Asp Leu Asn Val Lys Pro Lys Thr Ile Lys Thr 965 970 975 Leu Glu Glu Asn Leu Gly Ile Thr Ile Gln Asp Ile Gly Val Gly Lys 980 985 990 Asp Phe Met Ser Lys Thr Pro Lys Ala Met Ala Thr Lys Asp Lys Ile 995 1000 1005 Asp Lys Trp Asp Leu Ile Lys Leu Lys Ser Phe Cys Thr Ala Lys 1010 1015 1020 Glu Thr Thr Ile Arg Val Asn Arg Gln Pro Thr Thr Trp Glu Lys 1025 1030 1035 Ile Phe Ala Thr Tyr Ser Ser Asp Lys Gly Leu Ile Ser Arg Ile 1040 1045 1050 Tyr Asn Glu Leu Lys Gln Ile Tyr Lys Lys Lys Thr Asn Asn Pro 1055 1060 1065 Ile Lys Lys Trp Ala Lys Asp Met Asn Arg His Phe Ser Lys Glu 1070 1075 1080 Asp Ile Tyr Ala Ala Lys Lys His Met Lys Lys Cys Ser Ser Ser 1085 1090 1095 Leu Ala Ile Arg Glu Met Gln Ile Lys Thr Thr Met Arg Tyr His 1100 1105 1110 Leu Thr Pro Val Arg Met Ala Ile Ile Lys Lys Ser Gly Asn Asn 1115 1120 1125 Arg Cys Trp Arg Gly Cys Gly Glu Ile Gly Thr Leu Leu His Cys 1130 1135 1140 Trp Trp Asp Cys Lys Leu Val Gln Pro Leu Trp Lys Ser Val Trp 1145 1150 1155 Arg Phe Leu Arg Asp Leu Glu Leu Glu Ile Pro Phe Asp Pro Ala 1160 1165 1170 Ile Pro Leu Leu Gly Ile Tyr Pro Glu Asp Tyr Lys Ser Cys Cys 1175 1180 1185 Tyr Lys Asp Thr Cys Thr Arg Met Phe Ile Ala Ala Leu Phe Thr 1190 1195 1200 Ile Ala Lys Thr Trp Asn Gln Pro Lys Cys Pro Thr Met Ile Asp 1205 1210 1215 Trp Ile Lys Lys Met Trp His Ile Tyr Thr Met Glu Tyr Tyr Ala 1220 1225 1230 Ala Ile Lys Asn Asp Glu Phe Ile Ser Phe Val Gly Thr Trp Met 1235 1240 1245 Lys Leu Glu Thr Ile Ile Leu Ser Lys Leu Ser Gln Glu Gln Lys 1250 1255 1260 Thr Lys His Arg Ile Phe Ser Leu Ile Gly Gly Asn 1265 1270 1275 <210> SEQ ID NO 4 <211> LENGTH: 830 <212> TYPE: DNA <213> ORGANISM: Homo Sapiens <400> SEQUENCE: 4 agttttttct taaatggtat aatatccagt atgaagtatt actgaatttg agtaatcatt 60 aacaaatata tttcactgcc atactgtata ccaggctttc ttctagggcc cagaaatata 120 agctggttaa gatccttgat tgattgagat tacattctaa caggtacagt agacttaata 180 gctaatatca gaaaagatta gcagatttat tcactgtgtt atttgtactt ttattctcca 240 tttgccttac cctgtatttg aagaaagttt tgccttgctt tttgatgtga atgaaattaa 300 gcttggattt cacaaccgtg gttgaattta agaaatgttc tatttttaca tggggaagac 360 ggtgctcaag taatacttgc aggtactagc acccaggatt taggagtcca gtccagtttt 420 agctacacaa aagtcttaag tacacaaatt gccaatagag cagaactata taattcatag 480 atttgctcat tattaatctc aaggaaatca gctctttaaa tatatgtatt taatgaatgt 540 gaaatttttg ggaaggggaa ctactatgta ttaagccata atatttattt tacttaaaaa 600 atttttaaac aaagtaatac tagtcattgt gagaatgcta ttctaaaaaa aaaaaaagtc 660 ccctggccac cttctctttc catccctaga gaccgaacat tttcaaaatt tgtagctact 720 tcttctactt agcctccatg tattaaacta atatgtgtaa taagaataat ccgggggagg 780 agccaagatg gccgaatagg aacagctccg gtctacagct cccagcgtga 830 <210> SEQ ID NO 5 <211> LENGTH: 1103 <212> TYPE: DNA <213> ORGANISM: Homo sapiens <400> SEQUENCE: 5 gggaggagcc aagatggccg aataggaaca gctccggtct acagctccca gcgtgagcga 60 cgcagaagac gggtgatttc tgcatttcca tctgaggtac cgggttcatc tcactaggga 120 gtgccagaca gtgggcgcag gccagtgtgt gtgcgcaccg tgcgcgagcc gaagcagggc 180 gaggcattgc ctcacctggg aagcgcaagg ggtcagggag ttccctttcc gagtcaaaga 240 aaggggtgac ggacgcacct ggaaaatcgg gtcactccca cccgaatatt gcgcttttca 300 gaccggctta agaaacggcg caccacgaga ctatatccca cacctggctc agagggtcct 360 acgcccacgg aatctcgctg attgctagca cagcagtctg agatcaaacg gcaaggcggc 420 aacgaggctg ggggaggggc gcccgccatt gcccaggctt gcttaggcaa acaaagcagc 480 tgggaagctc gaactgggtg gagcccacca cagctcaagg aggcctgcct gcctctgtag 540 gctccacctc tgggggcagg gcacagacaa acaaaaagac agcagtaacc tctgcagact 600 taagtgtccc tgtctgacag ctttgaagag agcagtggtt ctcccagcac gcagctggag 660 atctgagaac gggcagactg cctcctcaag tgggtccctg acccctgacc cccgagcagc 720 ctaactggga ggcacccccc agcagggcac actgacacct cacacagcag ggtattccaa 780 cagacctgca gctgagggtc ctgtctgtta gaaggaaaac taacaaccag aaaggacatc 840 tacaccgaaa acccatctgt acatcaccat catcaaagac caaaagtaga taaaaccaca 900 aagatgggga aaaaacagaa cagaaaaact ggaaactcta aaacgcagag cgcctctcct 960 cctccaaagg aacgcagttc ctcaccagca acagaacaaa gctggatgga gaatgatttt 1020 gacgagctga gagaagaagg cttcagacga tcaaattact ctgagctacg ggaggacatt 1080 caaaccaaag gcaaagaagt tga 1103 <210> SEQ ID NO 6 <211> LENGTH: 1104 <212> TYPE: DNA <213> ORGANISM: Homo sapiens <400> SEQUENCE: 6 gggaggagcc aagatggccg aataggaaca gctccggtct acagctccca gcgtgagcga 60 cgcagaagac gggtgatttc tgcatttcca tctgaggtac cgggttcatc tcactaggga 120 gtgccagaca gtgggcgcag gccagtgtgt gtgcgcaccg tgcgcgagcc gaagcagggc 180 gaggcattgc ctcacctggg aagcgcaagg ggtcagggag ttcccttttc gagtcaaaga 240 aaggggtgac ggacgcacct ggaaaatcgg gtcactccca cccgaatatt gcgcttttca 300 gaccggctta agaaacggcg caccacgaga ctatatccca cacctggctc agagggtcct 360 acgcccacgg aatctcgctg attgctagca cagcagtctg agatcaaacg gcaaggcggc 420 aacgaggctg ggggaggggc gcccgccatt gcccaggctt gcttaggcaa acaaagcagc 480 tgggaagctc gaactgggtg gagcccacca cagctcaagg aggcctgcct gcctctgtag 540 gctccacctc tgggggcagg gcacagacaa acaaaaagac agcagtaacc tctgcagact 600 taagtgtccc tgtctgacag ctttgaagag agcagtggtt ctcccagcac gcagctggag 660 atctgagaac gggcagactg cctcctcaag tgggtccctg acccctgacc cccgagcagc 720 ctaactggga ggcacccccc agcaggggca cactgacacc tcacacagca gggtattcca 780 acagacctgc agctgagggt cctgtctgtt agaaggaaaa ctaacaacca gaaaggacat 840 ctacaccgaa aacccatctg tacatcacca tcatcaaaga ccaaaagtag ataaaaccac 900 aaagatgggg aaaaaacaga acagaaaaac tggaaactct aaaacgcaga gcgcctctcc 960 tcctccaaag gaacgcagtt cctcaccagc aacagaacaa agctggatgg agaatgattt 1020 tgacgagctg agagaagaag gcttcagacg atcaaattac tctgagctac gggaggacat 1080 tcaaaccaaa ggcaaagaag ttga 1104 <210> SEQ ID NO 7 <211> LENGTH: 600 <212> TYPE: DNA <213> ORGANISM: Homo Sapiens <400> SEQUENCE: 7 ctcgctctcc acgggttgga cccactgcct aaccatttcc agtgagatga actggttacc 60 tcagttggag atacagaaat caccagcctt ctgagttggt ctcgctggga gctgcagacc 120 agagctgttc ctatttagcc atcttggccc ctcccccctt gaaaattcca tttctttaat 180 agatataggg ctattgaggc tatttctcct taaatgaacc tagatagttt gtgtgcagct 240 gtcaaggaat ttgtccattt tatctaagtt gtcatattta tctatataaa gtttttcata 300 atattcgttt attatctatt taccgtctat agcagtactg atggcttttg aatactagca 360 cggctaattg caaatctata gtcatgtcac ctgtctcatt cctaagattt aaaaatgcac 420 tgcaggacac aaagttattc cacacacctc gacttagctt atttgtgtat ttcttccaag 480 agaaaaaaaa aaaagaggcc aggcatggtg gctcacgcct gtaatcccag cactttggga 540 ggctgaggca ggtggatcac tttaggtcag gagtttgaga tcagcctggc caacatggcg 600 <210> SEQ ID NO 8 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: PCR primer <400> SEQUENCE: 8 ctgccatact gtataccagg 20 <210> SEQ ID NO 9 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: PCR primer <400> SEQUENCE: 9 ctgttcctat tcggccatct 20 <210> SEQ ID NO 10 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: PCR primer <400> SEQUENCE: 10 ctagggccca gaaatataag 20 <210> SEQ ID NO 11 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: PCR primer <400> SEQUENCE: 11 ccccggatta ttcttattac 20 <210> SEQ ID NO 12 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: PCR primer <400> SEQUENCE: 12 ctggttacct cagttggaga 20 <210> SEQ ID NO 13 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: PCR primer <400> SEQUENCE: 13 atgttggcca ggctgatctc 20 <210> SEQ ID NO 14 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: PCR primer <400> SEQUENCE: 14 agccttctga gttggtctcg 20 <210> SEQ ID NO 15 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: PCR primer <400> SEQUENCE: 15 agtgatccac ctgcctcagc 20 

What is claimed is:
 1. A method of identifying a gene involved in a complex disease comprising the steps of: (i) identifying a region of the genome neighboring a disease-associated marker; (ii) comparing the sequence of the 5′ regulatory region of a consensus L1 sequence to the intronic region of genes or predicted genes or to the 5′ or 3′ regulatory region of genes or predicted genes; and (iii) identifying genes containing a full-length L1 element in their intronic region or containing a full-length L1 element with high sequence fidelity to the L1 consensus sequence in their 5′ or 3′ regulatory region, wherein said genes identified in step (iii) are involved in a complex disease.
 2. The method of claim 1, wherein the high fidelity L1 sequence is at least about 95% similar to the sequence of nucleotides 1-884 of SEQ ID NO:
 1. 3. The method of claim 1, wherein the complex disease is selected from the group consisting of systemic lupus erythematosus, multiple sclerosis, insulin-dependent diabetes mellitus, rheumatoid arthritis, phemphigus, psoriasis, autoimmune thyroid disease, scleroderma, mixed connective tissue disease, polymyositis, dermatomyositis, Sjögren's syndrome, pemphigoid, vitiligo, primary biliary cirrhosis, chronic active hepatitis, Crohn's disease, ulcerative colitis, pernicious anemia, schizophrenia, and Alzheimer disease.
 4. A method of identifying an individual at risk for or suffering from a complex disease comprising the steps of: (i) providing a sample from said individual; (ii) identifying intronic regions of genes containing full-length L1 elements or in 5′ or 3′ regulatory regions of genes containing a full-length high fidelity consensus L1 sequence of the individual's DNA from said sample; and (iii) comparing said intronic regions of genes or said 5′ or 3′ regulatory regions of step (ii) with a control sample of DNA taken from an individual not susceptible to or at risk for or currently suffering from a complex disease wherein said genes identified in step (ii) are involved in a complex disease
 5. The method of claim 4, wherein the complex disease is selected from the group consisting of systemic lupus erythematosus, multiple sclerosis, insulin-dependent diabetes mellitus, rheumatoid arthritis, phemphigus, psoriasis, autoimmune thyroid disease, scleroderma, mixed connective tissue disease, polymyositis, dermatomyositis, Sjögren's syndrome, pemphigoid, vitiligo, primary biliary cirrhosis, chronic active hepatitis, Crohn's disease, ulcerative colitis, pernicious anemia, schizophrenia, and Alzheimer disease.
 6. The method of claim 4, wherein said sample is blood, serum, saliva, urine, tears, sweat, synovial fluid, cerebrospinal fluid, or a solid tissue.
 7. A method of identifying an individual at risk for or suffering from a complex disease comprising the steps of: a. providing a sample from said individual suffering from a complex disease; b. detecting the amount of L1 DNA, mRNA or a protein encoded by an L1 element in said sample; c. comparing the amount of step (ii) with an amount of L1DNA, mRNA or a protein obtained from an individual not susceptible to or at risk for or suffering from a complex disease, wherein if the amount detected in said sample obtained from said individual is greater than the amount of said control, said individual is at risk for or suffering from a complex disease.
 8. The method of claim 7, wherein said sample is blood, serum, saliva, urine, tears, sweat, synovial fluid, cerebrospinal fluid, or a solid tissue.
 9. The method of claim 7, wherein the complex disease is selected from the group consisting of systemic lupus erythematosus, multiple sclerosis, insulin-dependent diabetes mellitus, rheumatoid arthritis, phemphigus, psoriasis, autoimmune thyroid disease, scleroderma, mixed connective tissue disease, polymyositis, dermatomyositis, Sjögren's syndrome, pemphigoid, vitiligo, primary biliary cirrhosis, chronic active hepatitis, Crohn's disease, ulcerative colitis, pernicious anemia, schizophrenia, and Alzheimer disease.
 10. A method of treating or preventing a complex disease, which method comprises administering a therapeutically effective amount of an agent selected from the group consisting of an L1 antisense oligonucleotide, an antibody directed against L1 mRNA, and an antibody directed against a protein encoded by an L1 element.
 11. The method of claim 10, wherein the complex disease is selected from the group consisting of systemic lupus erythematosus, multiple sclerosis, insulin-dependent diabetes mellitus, rheumatoid arthritis, phemphigus, psoriasis, autoimmune thyroid disease, scleroderma, mixed connective tissue disease, polymyositis, dermatomyositis, Sjögren's syndrome, pemphigoid, vitiligo, primary biliary cirrhosis, chronic active hepatitis, Crohn's disease, ulcerative colitis, pernicious anemia, schizophrenia, and Alzheimer disease.
 12. A method for identifying an individual at risk for or suffering from a complex disease comprising the steps of: (i) providing a sample obtained from said individual; (ii) detecting antibodies directed against ribonucleo-protein particles having L1 mRNA complements in said sample wherein said individual is at risk for or is suffering from a complex disease if said antibodies are present in said sample.
 13. The method of claim 12, wherein the complex disease is selected from the group consisting of systemic lupus erythematosus, multiple sclerosis, insulin-dependent diabetes mellitus, rheumatoid arthritis, phemphigus, psoriasis, autoimmune thyroid disease, scleroderma, mixed connective tissue disease, polymyositis, dermatomyositis, Sjögren's syndrome, pemphigoid, vitiligo, primary biliary cirrhosis, chronic active hepatitis, Crohn's disease, ulcerative colitis, pernicious anemia, schizophrenia, and Alzheimer disease.
 14. The method of claim 12, wherein said sample is blood, serum, saliva, urine, tears, sweat, synovial fluid, cerebrospinal fluid, or a solid tissue.
 15. A method of identifying an individual at risk for or suffering from a complex disease comprising the steps of: (i) providing a sample obtained from said individual; (ii) analyzing said sample for the presence of auto antibodies directed against L1 DNA, nRNA or protein products wherein said individual is at risk for or suffering from a complex disease if said antibodies are present in said sample.
 16. The method of claim 15, wherein said sample is blood, serum, saliva, urine, tears, sweat, synovial fluid, cerebrospinal fluid, or a solid tissue.
 17. The method of claim 15, wherein the complex disease is selected from the group consisting of systemic lupus erythematosus, multiple sclerosis, insulin-dependent diabetes mellitus, rheumatoid arthritis, phemphigus, psoriasis, autoimmune thyroid disease, scleroderma, mixed connective tissue disease, polymyositis, dermatomyositis, Sjögren's syndrome, pemphigoid, vitiligo, primary biliary cirrhosis, chronic active hepatitis, Crohn's disease, ulcerative colitis, pernicious anemia, schizophrenia, and Alzheimer disease.
 18. The method of claim 15, wherein the complex disease is systemic lupus erythematosus. 